ACL_RS04045

Uniprot: A9NGF2
Description: signal peptidase II
EC number: 3.4.23.36
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. {ECO:0000256|HAMAP-Rule:MF_00161, ECO:0000256|RuleBase:RU000594, ECO:0000256|SAAS:SAAS00112457}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: This protein specifically catalyzes the removal of signal peptides from prolipoproteins. {ECO:0000256|HAMAP-Rule:MF_00161, ECO:0000256|RuleBase:RU000594, ECO:0000256|SAAS:SAAS00112518}.
Pathway: PATHWAY: Protein modification; lipoprotein biosynthesis (signal peptide cleavage). {ECO:0000256|HAMAP-Rule:MF_00161, ECO:0000256|SAAS:SAAS00112477}.
Active site: ACT_SITE 104 104 {ECO:0000256|HAMAP-Rule:MF_00161}.; ACT_SITE 132 132 {ECO:0000256|HAMAP-Rule:MF_00161}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): lspA
Gene names (synonym):
Mass: 18,466
Subunit structure [CC]:
Gene ontology (GO): integral component of membrane [GO:0016021]; plasma membrane [GO:0005886]; aspartic-type endopeptidase activity [GO:0004190]
Gene ontology IDs: GO:0004190; GO:0005886; GO:0016021
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the peptidase A8 family. {ECO:0000256|HAMAP-Rule:MF_00161, ECO:0000256|RuleBase:RU004181, ECO:0000256|SAAS:SAAS00581686}.
Protein families: Peptidase A8 family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal:
MEROPS: A08.001
EnsemblBacteria KO: ABX81432
UniPathway: K03101
CDD:
Gene3D:
HAMAP:
InterPro: MF_00161
PANTHER: IPR001872
PIRSF: PTHR33695:SF1
PRINTS:
PROSITE: PR00781
Pfam: PS00855
ProDom: PF01252
SMART:
SUPFAM:
TIGRFAMs:

853311-853809(-)

>nucleotide sequence
TTAAATTTCATTTTGTGTATCCTTCTTTATCTTTTTCTGTCTTAGTGTGTCTAGTATTAA
TATATCAATCAGTAGTAAAACAACACCAGCATTTAATAGTACGTCTGCAAAATTGAATAT
TGTTCCACCCACAATTGGAAGAAATGGCATTTGAACATAGTCAATGACATAACCAAATAA
TACGCGGTCGATTGCATTGCCTAATGTACCACTAATCAGTAATATAAAAGCAATCGTATA
AACTTTCTTTGTTTTCCAATCTGATTTTGAGAAAAAATATCCAAATAATACTAAAGCAAA
CAAGGTAATGATGAAAAATAAAAATTGTTGTCCTTGAAGTAACCCAAAGGAGGCTCCAGT
ATTTTGTACATAGCCTACATATAGCGTATTATCTATTACAGATACAATACTTCCAGCTGC
ATACATATTTGAAAATATTTGTTTAAGTATGACATCTACTAAGATAAGTACTGCCATAAT
GATATACGATAAAATCAT

>protein sequence
MILSYIIMAVLILVDVILKQIFSNMYAAGSIVSVIDNTLYVGYVQNTGASFGLLQGQQFL
FFIITLFALVLFGYFFSKSDWKTKKVYTIAFILLISGTLGNAIDRVLFGYVIDYVQMPFL
PIVGGTIFNFADVLLNAGVVLLLIDILILDTLRQKKIKKDTQNEI