ACL_RS04075

Uniprot: A9NGF8
Description: histidine--tRNA ligase
EC number: 6.1.1.21
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). {ECO:0000255|HAMAP-Rule:MF_00127}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): hisS
Gene names (synonym):
Mass: 48,170
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00127}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; histidine-tRNA ligase activity [GO:0004821]; histidyl-tRNA aminoacylation [GO:0006427]
Gene ontology IDs: GO:0004821; GO:0005524; GO:0005737; GO:0006427
Chain: CHAIN 1 420 Histidine--tRNA ligase. /FTId=PRO_1000076258.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. {ECO:0000255|HAMAP-Rule:MF_00127}.
Protein families: Class-II aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NGF8
MEROPS:
EnsemblBacteria KO: ABX81438
UniPathway: K01892
CDD:
Gene3D: cd00859
HAMAP: 3.40.50.800
InterPro: MF_00127
PANTHER: IPR006195;IPR004154;IPR015807;IPR004516;IPR033656
PIRSF: PTHR11476
PRINTS: PIRSF001549
PROSITE:
Pfam: PS50862
ProDom: PF03129
SMART:
SUPFAM:
TIGRFAMs: SSF52954

857815-859078(-)

>nucleotide sequence
TTATAATAGCTCCTTTAGTGTATCAAGTAAGGTATCCATGGATACTTCGACTTGTTCTTC
TGTTTTGGTGTTTTTTAATTGTACTACATTGTTATTTAATTCATTTTCACCAATAATGAT
GATGAATTTAGAATCATATCTTAAAGCTTCTTTAAGTTGAGGTTTAAATCCTCTATTTAA
GTGATCGATATCAGATAAAATGTTTTCACTTCTTAAAATGTGTTGTAACTGGATTGCCTT
TTTTCTACTTTGTTGATCAAATGTAATGAAATAGACATCCAATTGTTTAGGACTAGTCAG
TGTAATACCTTCTTGTTCTAGTGCACTAAGTAATCTTTCCATACCAAAGGCATAACCAAT
ACCTGGTGTATCTGGTCCACCTAATTCTTTAACTAGGTTTTGGTATCTACCACCACCACC
TAAGGCATTTTGGGCACCAAATCCTTTAATTGTTGCATGAATTTCAAATACTGTATGTGC
ATAGTAATCTAGTCCACGGACTAATTTTCTATCAATTTCGTATGGGATATTCATAAGGTT
TAGTAATTCAAGTACTGAATCAAAATGTTGCTTTGATGTTTCATTTAAGTAATCTAATGG
TAATGGAGCATTTTTAATGAGTTCAATGTCTGCTGTATCCTTAGAATCCAGTATACGTAG
CGGATTATGAGTTAGTCTATCTTGAGAATCCTTAGATAATTTATCCTTTACAGGTGTTAA
AAAATCAATAAGTGCTTGGCGGTAGTTTGCTTTTGAAGCATCATCACCTAAACTGTTAAG
TTTAACACTTACTTGTTTTAAACCTAAACGATAAATTGTTTCATAAGCTAGTTCAATGAC
TTCTGCATCAATACTTGGTTCATTTGAACCTAAGACTTCACAACCAAACTGCATAAATTG
TCTAAAGCGACCTTTTTGAGGACGTTCATATCTAAAATTAGGTCCCATATAATAAAGTTT
AGTCACACCAGCTTGAGCATAAAGTTTATTTTCAACATAACTACGAATCACACCTGCTGT
ACCTTCTGGTCTTAAAGTTAAATCACGATCTGATTTATCTTTAAAGTTATAAGTTTCTTT
GATAACCATATCAGACAATTCAGTTTCACGGTGAATTACATTAGAATATTCCATCATCGG
TGTTCTAATTTCACCATAGTTATATGTTTTAAATAGTTTCCTGACATAGGATTCTAAAGC
AACCCAATTTTCTGCTTCAGTAGGTAATACATCGTATGTACCCTTAACTTTTATATAGTT
CAT

>protein sequence
MNYIKVKGTYDVLPTEAENWVALESYVRKLFKTYNYGEIRTPMMEYSNVIHRETELSDMV
IKETYNFKDKSDRDLTLRPEGTAGVIRSYVENKLYAQAGVTKLYYMGPNFRYERPQKGRF
RQFMQFGCEVLGSNEPSIDAEVIELAYETIYRLGLKQVSVKLNSLGDDASKANYRQALID
FLTPVKDKLSKDSQDRLTHNPLRILDSKDTADIELIKNAPLPLDYLNETSKQHFDSVLEL
LNLMNIPYEIDRKLVRGLDYYAHTVFEIHATIKGFGAQNALGGGGRYQNLVKELGGPDTP
GIGYAFGMERLLSALEQEGITLTSPKQLDVYFITFDQQSRKKAIQLQHILRSENILSDID
HLNRGFKPQLKEALRYDSKFIIIIGENELNNNVVQLKNTKTEEQVEVSMDTLLDTLKELL