ACL_RS04295

Uniprot: A9NGK2
Description: non-canonical purine NTP pyrophosphatase
EC number: 3.6.1.19
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: A nucleoside triphosphate + H(2)O = a nucleotide + diphosphate. {ECO:0000255|HAMAP-Rule:MF_01405}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01405}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_01405}; ; Note=Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+). {ECO:0000255|HAMAP-Rule:MF_01405}
Enzyme regulation:
Function [CC]: FUNCTION: Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions. {ECO:0000255|HAMAP-Rule:MF_01405}.
Pathway:
Active site:
Binding site: BINDING 150 150 Substrate. {ECO:0000255|HAMAP-Rule:MF_01405}.; BINDING 170 170 Substrate. {ECO:0000255|HAMAP-Rule:MF_01405}.; BINDING 176 176 Substrate. {ECO:0000255|HAMAP-Rule:MF_01405}.
Calcium binding:
DNA binding:
Metal binding: METAL 68 68 Magnesium or manganese. {ECO:0000255|HAMAP-Rule:MF_01405}.
Nucleotide binding:
Site:
Gene names (primary):
Gene names (synonym):
Mass: 22,736
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01405}.
Gene ontology (GO): metal ion binding [GO:0046872]; nucleoside-triphosphatase activity [GO:0017111]; nucleoside-triphosphate diphosphatase activity [GO:0047429]; nucleotide binding [GO:0000166]; nucleoside triphosphate catabolic process [GO:0009143]; purine nucleotide metabolic process [GO:0006163]
Gene ontology IDs: GO:0000166; GO:0006163; GO:0009143; GO:0017111; GO:0046872; GO:0047429
Chain: CHAIN 1 200 Non-canonical purine NTP pyrophosphatase. /FTId=PRO_1000087375.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the HAM1 NTPase family. {ECO:0000255|HAMAP-Rule:MF_01405}.
Protein families: HAM1 NTPase family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 7 12 Substrate binding. {ECO:0000255|HAMAP-Rule:MF_01405}.; REGION 68 69 Substrate binding. {ECO:0000255|HAMAP-Rule:MF_01405}.
EMBL: CP000896
ProteinModelPortal: A9NGK2
MEROPS:
EnsemblBacteria KO: ABX81482
UniPathway: K02428
CDD:
Gene3D: cd00515
HAMAP: 3.90.950.10
InterPro: MF_01405
PANTHER: IPR002637;IPR029001;IPR020922
PIRSF: PTHR11067
PRINTS:
PROSITE:
Pfam:
ProDom: PF01725
SMART:
SUPFAM:
TIGRFAMs: SSF52972

899202-899805(-)

>nucleotide sequence
TCATTTCTTCCATGTATATCGCCAGTAATCAATAATTTCATCTTTAGCCTCCAAAAAGTT
GTTCAAAGCATGTCTTCTATGCGAATGTTCATCTTTATAAGTTGAACCTAATTCACCCAG
TGTTTCTTGCTTACCATCTGGTATGAAGATTGGATCATAACCAAATCCCATACTCCCTTT
TAAGTTTAAAGCTATATTGCCTAACATGTTGCCAACGTATGTAAATATTTTACCATCAGG
AAACGCAATTGCAATCGCACATACAAATCTTGCTTGACGATTTTCCTTATTTTTTAGTAC
ATCTAATACTTTAATATTATTCACAGTATCACCTAATCCACTATACCGTTTCGAATAGAT
CCCTGGTAGGGGACTTATCGCTTCTATAATGATACCAGAATCATCTGCAATGGCCACTTG
ATTAAATCTTTTAGCAAATGCTCGTGCTTTAATCTGTGCATTTGCTTCAAAGGTTAATCC
GGATTCTATAATTTCTTTTTCATCTATTTGAAAGTCTTTTAATAAAGTGATCTGATGTGG
TTTTAAAATACTTTCCATTTCTATGAACTTATGATAATTATTTGATGCAAATATTATATC
CAT

>protein sequence
MDIIFASNNYHKFIEMESILKPHQITLLKDFQIDEKEIIESGLTFEANAQIKARAFAKRF
NQVAIADDSGIIIEAISPLPGIYSKRYSGLGDTVNNIKVLDVLKNKENRQARFVCAIAIA
FPDGKIFTYVGNMLGNIALNLKGSMGFGYDPIFIPDGKQETLGELGSTYKDEHSHRRHAL
NNFLEAKDEIIDYWRYTWKKW