ACL_RS04315

Uniprot: A9NGK6
Description: glycerol-3-phosphate dehydrogenase (NAD(P)(+))
EC number: 1.1.1.94
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(P)(+) = glycerone phosphate + NAD(P)H. {ECO:0000255|HAMAP-Rule:MF_00394}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Membrane lipid metabolism; glycerophospholipid metabolism. {ECO:0000255|HAMAP-Rule:MF_00394}.
Active site: ACT_SITE 188 188 Proton acceptor. {ECO:0000255|HAMAP-Rule:MF_00394}.
Binding site: BINDING 101 101 NAD; via amide nitrogen. {ECO:0000255|HAMAP-Rule:MF_00394}.; BINDING 101 101 Substrate. {ECO:0000255|HAMAP-Rule:MF_00394}.; BINDING 136 136 NAD; via amide nitrogen. {ECO:0000255|HAMAP-Rule:MF_00394}.; BINDING 252 252 NAD. {ECO:0000255|HAMAP-Rule:MF_00394}.; BINDING 278 278 NAD. {ECO:0000255|HAMAP-Rule:MF_00394}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 7 12 NAD. {ECO:0000255|HAMAP-Rule:MF_00394}.
Site:
Gene names (primary): gpsA
Gene names (synonym):
Mass: 36,225
Subunit structure [CC]:
Gene ontology (GO): glycerol-3-phosphate dehydrogenase complex [GO:0009331]; glycerol-3-phosphate dehydrogenase [NAD(P)+] activity [GO:0047952]; glycerol-3-phosphate dehydrogenase [NAD+] activity [GO:0004367]; glycerol-3-phosphate dehydrogenase [NADP+] activity [GO:0036439]; NAD binding [GO:0051287]; carbohydrate metabolic process [GO:0005975]; glycerol-3-phosphate biosynthetic process [GO:0046167]; glycerol-3-phosphate catabolic process [GO:0046168]; glycerophospholipid metabolic process [GO:0006650]; phospholipid biosynthetic process [GO:0008654]
Gene ontology IDs: GO:0004367; GO:0005975; GO:0006650; GO:0008654; GO:0009331; GO:0036439; GO:0046167; GO:0046168; GO:0047952; GO:0051287
Chain: CHAIN 1 331 Glycerol-3-phosphate dehydrogenase [NAD(P)+]. /FTId=PRO_1000080296.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. {ECO:0000255|HAMAP-Rule:MF_00394}.
Protein families: NAD-dependent glycerol-3-phosphate dehydrogenase family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 252 253 Substrate binding. {ECO:0000255|HAMAP-Rule:MF_00394}.
EMBL: CP000896
ProteinModelPortal: A9NGK6
MEROPS:
EnsemblBacteria KO: ABX81486
UniPathway: K00057
CDD:
Gene3D:
HAMAP: 1.10.1040.10;3.40.50.720
InterPro: MF_00394
PANTHER: IPR008927;IPR013328;IPR006168;IPR006109;IPR011128;IPR016040
PIRSF:
PRINTS: PIRSF000114
PROSITE: PR00077
Pfam:
ProDom: PF07479;PF01210
SMART:
SUPFAM:
TIGRFAMs: SSF48179;SSF51735

902780-903776(-)

>nucleotide sequence
TTAAGCAATAACTTCTTCTTTTAACTCTCTAGTTAATAGATTTTGAATCGCTTCTTTGAC
ACTCATGTTATTAAAGATAACCTCATAAGTGATTTCAATAATAGGTAACTCAATTCCTGT
TTGAACACATAAATCTTTTGCTGCTTGGATTGCACGAACACCTTCTACAACCATCTTAGA
TTCACTTAAAACTTGTTCAACTGGTGTACCTTGACCTATCTTTAAACCTGCATTAAAGTT
TCTAGAATTATAACTTGAAGCTGTTACAATTAAATCTCCAATACCTGTTAAGCCAAACGC
AGTTTCTTTTTTACCACCATAGTGAACAACAACACGTACAATTTCTAGGATCCCACGTGT
AATTAATGCTGCACGTGCATTTTCTCCTAAACCATAACCTGTCATCATACCCGATACGAT
AGCAATCGCATTTTTAATTGCACCACCGACTTCACAGCCAATTAAATCATCTGATGTATA
AACACGTAAGTAGCTTTGATTTGAAAAGAGTTGTTGAACACTTTTTCTAAACCATTCCAC
ATCACTTGCAGCAGTTAATACTGTAAGTTTACGTTCGATAACTTCTTCAGCATGAGATGG
CCCTGTCAATACTGCGTAAGCACCAAGTAATTCTGGGGTAATTTCATCAGTTACAATTTC
TGAAACACGTTTAAGTGTTACAGGTTCAATTCCTTTAGATACATTGATAAAATATAGTTT
TCTAGTTGCTAGTTTATTAATATCTCTTAAGGAGTCACGCATAAACTTAGTAGGCACTGC
AAGTAAAATGTAATCAGCATAATCAATAGATTCTTTCAAATCTAATGTGGCACGAATGCC
AGTAATCTTATTATCGAAAAATGGATGGATATTATTGTTAATCTTTTTAACTGCTTCAGG
ATTCACATCATAAATCAATGTTTCATGTCCATTATCAGTTAGTACTTGAGCAAGTGTAGT
TCCCCAAGAACCGCCACCAATTACACTAATCTTCAT

>protein sequence
MKISVIGGGSWGTTLAQVLTDNGHETLIYDVNPEAVKKINNNIHPFFDNKITGIRATLDL
KESIDYADYILLAVPTKFMRDSLRDINKLATRKLYFINVSKGIEPVTLKRVSEIVTDEIT
PELLGAYAVLTGPSHAEEVIERKLTVLTAASDVEWFRKSVQQLFSNQSYLRVYTSDDLIG
CEVGGAIKNAIAIVSGMMTGYGLGENARAALITRGILEIVRVVVHYGGKKETAFGLTGIG
DLIVTASSYNSRNFNAGLKIGQGTPVEQVLSESKMVVEGVRAIQAAKDLCVQTGIELPII
EITYEVIFNNMSVKEAIQNLLTRELKEEVIA