ACL_RS04345


  Uniprot: A9NGL2
Description: 3-dehydroquinate dehydratase
EC number: 4.2.1.10
Annotation score: 3 out of 5
Miscellaneous [CC]: 
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 3-dehydroquinate = 3-dehydroshikimate + H(2)O. {ECO:0000256|HAMAP-Rule:MF_00169, ECO:0000256|SAAS:SAAS00027731}.
Cofactor: 
Enzyme regulation: 
Function [CC]: FUNCTION: Catalyzes a trans-dehydration via an enolate intermediate. {ECO:0000256|HAMAP-Rule:MF_00169}.
Pathway: PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. {ECO:0000256|HAMAP-Rule:MF_00169}.
Active site: ACT_SITE 22 22 Proton acceptor. {ECO:0000256|HAMAP-Rule:MF_00169, ECO:0000256|PIRSR:PIRSR001399-1}.; ACT_SITE 95 95 Proton donor. {ECO:0000256|HAMAP-Rule:MF_00169, ECO:0000256|PIRSR:PIRSR001399-1}.
Binding site: BINDING 69 69 Substrate. {ECO:0000256|HAMAP-Rule:MF_00169}.; BINDING 75 75 Substrate. {ECO:0000256|HAMAP-Rule:MF_00169}.; BINDING 82 82 Substrate. {ECO:0000256|HAMAP-Rule:MF_00169}.; BINDING 106 106 Substrate. {ECO:0000256|HAMAP-Rule:MF_00169, ECO:0000256|PIRSR:PIRSR001399-2}.
Calcium binding: 
DNA binding: 
Metal binding: 
Nucleotide binding: 
Site: SITE 17 17 Transition state stabilizer. {ECO:0000256|HAMAP-Rule:MF_00169, ECO:0000256|PIRSR:PIRSR001399-3}.
Gene names (primary): aroQ
Gene names (synonym): 
Mass: 16,270
Subunit structure [CC]: SUBUNIT: Homododecamer. {ECO:0000256|HAMAP-Rule:MF_00169, ECO:0000256|SAAS:SAAS00351441}.
Gene ontology (GO): 3-dehydroquinate dehydratase activity [GO:0003855]; aromatic amino acid family biosynthetic process [GO:0009073]; chorismate biosynthetic process [GO:0009423]
Gene ontology IDs: GO:0003855; GO:0009073; GO:0009423
Chain: 
Signal peptide: 
Domain [CC]: 
Sequence similarities: SIMILARITY: Belongs to the type-II 3-dehydroquinase family. {ECO:0000256|HAMAP-Rule:MF_00169, ECO:0000256|SAAS:SAAS00545386}.
Protein families: Type-II 3-dehydroquinase family
Coiled coil: 
Domain [FT]: 
Motif: 
Region: REGION 96 97 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00169}.
EMBL: CP000896
ProteinModelPortal: A9NGL2
MEROPS: 
EnsemblBacteria KO: ABX81492
UniPathway: K03786
CDD: 
Gene3D: cd00466
HAMAP: 3.40.50.9100
InterPro: MF_00169
PANTHER: IPR001874;IPR018509
PIRSF: PTHR21272
PRINTS: PIRSF001399
PROSITE: 
Pfam: PS01029
ProDom: PF01220
SMART: PD004527
SUPFAM: 
TIGRFAMs: SSF52304
908659-909085(-)

>nucleotide sequence
CTAGAAATTACTCATTTTTTCAAGTATATAAGTAACTGCATCCATATAAGACTGCTCTTT
TTTACCAAAAAATGTTTGATTTACCACATCTTTTATATAATTTATCTTTCTAAAAGCTTC
TCGTTCATAAATATTTGATAAATGAACTTCCACTTTAATTAACTTAATTGTTTCTAAACA
ATCTCTAATAGCAATCGACGTATGTGTATATGCACCTGCATTGATTACGATAGCATCACA
TGCTGTTTCTTGAAGAAAATCAATGATTGCACCTTCATGATTACTTTGGAAAAATTCAAA
GTCTACTTTAGGAAATGTATTTTGAATATGACAATTGATATCATCAAGTGTCATCATGCC
ATAATGATTTACATCTCTTTTACCTAACATATTTAAATTTGGACCATGAACAATAGCTAT
CTTCAT

>protein sequence
MKIAIVHGPNLNMLGKRDVNHYGMMTLDDINCHIQNTFPKVDFEFFQSNHEGAIIDFLQE
TACDAIVINAGAYTHTSIAIRDCLETIKLIKVEVHLSNIYEREAFRKINYIKDVVNQTFF
GKKEQSYMDAVTYILEKMSNF






















© Fisunov Lab of Proteomics, 2016.