ACL_RS04350

Uniprot: A9NGL3
Description: shikimate kinase
EC number: 2.7.1.71
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + shikimate = ADP + shikimate 3-phosphate. {ECO:0000256|HAMAP-Rule:MF_00109, ECO:0000256|SAAS:SAAS00407784}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00109}; ; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00109}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. {ECO:0000256|HAMAP-Rule:MF_00109}.
Pathway: PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. {ECO:0000256|HAMAP-Rule:MF_00109, ECO:0000256|SAAS:SAAS00407745}.
Active site:
Binding site: BINDING 32 32 Substrate. {ECO:0000256|HAMAP-Rule:MF_00109}.; BINDING 56 56 Substrate. {ECO:0000256|HAMAP-Rule:MF_00109}.; BINDING 77 77 Substrate; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00109}.; BINDING 113 113 ATP. {ECO:0000256|HAMAP-Rule:MF_00109}.; BINDING 128 128 Substrate. {ECO:0000256|HAMAP-Rule:MF_00109}.
Calcium binding:
DNA binding:
Metal binding: METAL 14 14 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00109}.
Nucleotide binding: NP_BIND 10 15 ATP. {ECO:0000256|HAMAP-Rule:MF_00109}.
Site:
Gene names (primary): aroK
Gene names (synonym):
Mass: 18,746
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00109}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; magnesium ion binding [GO:0000287]; shikimate kinase activity [GO:0004765]; aromatic amino acid family biosynthetic process [GO:0009073]; chorismate biosynthetic process [GO:0009423]
Gene ontology IDs: GO:0000287; GO:0004765; GO:0005524; GO:0005737; GO:0009073; GO:0009423
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the shikimate kinase family. {ECO:0000256|HAMAP-Rule:MF_00109}.
Protein families: Shikimate kinase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NGL3
MEROPS:
EnsemblBacteria KO: ABX81493
UniPathway: K00891
CDD:
Gene3D: cd00464
HAMAP: 3.40.50.300
InterPro: MF_00109
PANTHER: IPR027417;IPR031322;IPR000623;IPR023000
PIRSF:
PRINTS:
PROSITE:
Pfam: PS01128
ProDom: PF01202
SMART:
SUPFAM:
TIGRFAMs: SSF52540

909081-909573(-)

>nucleotide sequence
TCATTTATTTTTCCAATCCGTAACAATCTTAACTAAGTCTGATACAGCTTTAGTTATATC
ATCACTGTTTGATACTATTTTATCAGCAAACCCTTGATATTTTAAAAATCTAATATCATA
AAGTTCTTCTAAGGTCATAGATTTTAATAAAGGTCTATCATAACTAGATTTTAATCTTTT
TTCTATTGTTTCAATCGGTGTATTAATATAAATTTTTAGTCCATCCATCAAAGCTTTGTT
TGCCTTCTTCATGACTATACCACCACCGGTTGAAATGACGTAATCTTTACCGATGAAATC
TTTTAGGCTTTCAGTTTCAATTTCTCTGAATTTTTCTTCGCCATACATTCCAAATATTTC
ATCAATGTAAAGTAAATATTTTTGTTCAATATACTGATCAAGATCGATAAATGTCTTATT
AAGTTCTACTGCTAACCTTTTACCTAACGTGGTTTTACCACTTCCCGGCATACCAATTAA
ATATATATTCAT

>protein sequence
MNIYLIGMPGSGKTTLGKRLAVELNKTFIDLDQYIEQKYLLYIDEIFGMYGEEKFREIET
ESLKDFIGKDYVISTGGGIVMKKANKALMDGLKIYINTPIETIEKRLKSSYDRPLLKSMT
LEELYDIRFLKYQGFADKIVSNSDDITKAVSDLVKIVTDWKNKW