ACL_RS04355

Uniprot: A9NGL4
Description: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA
EC number: 2.5.1.75
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA. {ECO:0000255|HAMAP-Rule:MF_00185}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00185};
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). {ECO:0000255|HAMAP-Rule:MF_00185}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 9 16 ATP. {ECO:0000255|HAMAP-Rule:MF_00185}.
Site: SITE 96 96 Interaction with substrate tRNA. {ECO:0000255|HAMAP-Rule:MF_00185}.
Gene names (primary): miaA
Gene names (synonym):
Mass: 32,142
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00185}.
Gene ontology (GO): ATP binding [GO:0005524]; tRNA dimethylallyltransferase activity [GO:0052381]; tRNA processing [GO:0008033]
Gene ontology IDs: GO:0005524; GO:0008033; GO:0052381
Chain: CHAIN 1 280 tRNA dimethylallyltransferase. /FTId=PRO_0000377042.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the IPP transferase family. {ECO:0000255|HAMAP-Rule:MF_00185}.
Protein families: IPP transferase family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 11 16 Substrate binding. {ECO:0000255|HAMAP-Rule:MF_00185}.; REGION 34 37 Interaction with substrate tRNA. {ECO:0000255|HAMAP-Rule:MF_00185}.
EMBL: CP000896
ProteinModelPortal: A9NGL4
MEROPS:
EnsemblBacteria KO: ABX81494
UniPathway: K00791
CDD:
Gene3D:
HAMAP: 3.40.50.300
InterPro: MF_00185
PANTHER: IPR018022;IPR027417
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF01715
SMART:
SUPFAM:
TIGRFAMs: SSF52540

909577-910420(-)

>nucleotide sequence
TTATACTCCGATAAATTCTTTGATGTCTTTTAAACACTCATCTAGCAAATCAGGGCTTAA
AGCATTATATACTTTAGGCTTCATTTGATTGATAAACCATGTTTTTTGTCTTTTAGCAAA
TCTCATTGTTGCAGTAATAATCTTTTCTTTAGCAGTGTCTAAATCATTCATACCATTTAA
ATAATCTTTGATTTCACGATAGCCTATAATATTTAAATCGTAGTTTATTAAAGCTTTAGT
CTCTTCAATAAATCCCTTTTCAATCATGAGATCTAATCTAGTTTCTAATCTTTTTTTCAA
GATTTTTCTATCCATATCCAAATAAATTAAATAGATGTCATAGAGTGGTTCGTTCTTTTT
AGTTTTGTTACTTCGCTTTTGACCACTGATGATTGTTCTATATGCAGATTCTATACGTCT
AGGATTATTTAGATCTATTTCAATATCCGGATCTGCTTTTCTAATAACTTCAATCATTTC
ATTAATGTTTGGAAATTCAACGTTATCTTGTTGCTCAAATTCATAATTATAAAGCGCGGA
CTTAACATATAAACCACTACCACCTACGATAAATGGTTTATCTATTTGATCAATTAGCGT
GCGAACATCTTGTTGAAAATTATAAACTGTATAGGGTTCATCTAAGGCTACATGACTGAT
TAAATGATGTTTTACACCATCCATTTCATCAGTTGTTATCTTAGCTGATCCAATATCTAG
TTTCTTATAAATACTTACACTATCACCATTAATAATTTCAGCGCCTAATGATTTGGCAAG
TTTTACAGATAATGCAGTCTTCCCACTACCTGTAGGTCCCACAATACAAACGACTTTTTT
CAT

>protein sequence
MKKVVCIVGPTGSGKTALSVKLAKSLGAEIINGDSVSIYKKLDIGSAKITTDEMDGVKHH
LISHVALDEPYTVYNFQQDVRTLIDQIDKPFIVGGSGLYVKSALYNYEFEQQDNVEFPNI
NEMIEVIRKADPDIEIDLNNPRRIESAYRTIISGQKRSNKTKKNEPLYDIYLIYLDMDRK
ILKKRLETRLDLMIEKGFIEETKALINYDLNIIGYREIKDYLNGMNDLDTAKEKIITATM
RFAKRQKTWFINQMKPKVYNALSPDLLDECLKDIKEFIGV