ACL_RS04670

Uniprot: A9NGS4
Description: DNA-directed RNA polymerase subunit sigma
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. {ECO:0000256|SAAS:SAAS00535554}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding: DNA_BIND 377 396 H-T-H motif. {ECO:0000256|HAMAP-Rule:MF_00963}.
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoD
Gene names (synonym): sigA
Mass: 48,668
Subunit structure [CC]: SUBUNIT: Interacts transiently with the RNA polymerase catalytic core. {ECO:0000256|HAMAP-Rule:MF_00963}.
Gene ontology (GO): cytoplasm [GO:0005737]; DNA binding [GO:0003677]; DNA-directed RNA polymerase activity [GO:0003899]; sigma factor activity [GO:0016987]; transcription factor activity, sequence-specific DNA binding [GO:0003700]; transcription initiation from bacterial-type RNA polymerase promoter [GO:0001123]
Gene ontology IDs: GO:0001123; GO:0003677; GO:0003700; GO:0003899; GO:0005737; GO:0016987
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities:
Protein families:
Coiled coil:
Domain [FT]: DOMAIN 207 220 RNA_pol_sigma70. {ECO:0000259|PROSITE:PS00715}.; DOMAIN 283 309 RNA_pol_sigma70. {ECO:0000259|PROSITE:PS00716}.; DOMAIN 376 402 RNA_pol_sigma70. {ECO:0000259|PROSITE:PS00716}.
Motif: MOTIF 207 210 Interaction with polymerase core subunit RpoC. {ECO:0000256|HAMAP-Rule:MF_00963}.
Region: REGION 183 253 Sigma-70 factor domain-2. {ECO:0000256|HAMAP-Rule:MF_00963}.; REGION 262 338 Sigma-70 factor domain-3. {ECO:0000256|HAMAP-Rule:MF_00963}.; REGION 351 404 Sigma-70 factor domain-4. {ECO:0000256|HAMAP-Rule:MF_00963}.
EMBL: CP000896
ProteinModelPortal: A9NGS4
MEROPS:
EnsemblBacteria KO: ABX81554
UniPathway: K03086
CDD:
Gene3D:
HAMAP: 1.10.10.10
InterPro: MF_00963
PANTHER: IPR014284;IPR000943;IPR009042;IPR007627;IPR007624;IPR007630;IPR013325;IPR013324;IPR012760;IPR028630;IPR011991
PIRSF:
PRINTS:
PROSITE: PR00046
Pfam: PS00715;PS00716
ProDom: PF00140;PF04542;PF04539;PF04545
SMART:
SUPFAM:
TIGRFAMs: SSF88659;SSF88946

969397-970657(-)

>nucleotide sequence
TCATTTTCTTGTAATATAAAATTCTTTTAATTTATTTTGACGTTGAGGACTACGTAATTT
TCTTAATGCTTTAGCTTCAATTTGACGGATACGTTCACGTGTAACACCAAACTCACGCCC
CACTTCTTCAAGTGTGTGTGTTTTACCATCTAATAGGCCATAACGTAATCTTAGGACCTT
TTCTTCTCTGTCAGTTAATGTTTCTAAAACTTCATCTAAGGTTTTCTTAACCATTTCTTG
CATCATATAATCATGTGGATTTAAAGCATTTGGATCGCTAATAAAATCCCCTAATGAAGA
ATCTTCTTCTTCACCAACTGGTGCTTCAAGTGAGATTGGTTCTTTTGCAATTCTTTGGAT
GTTTTGAACTTTTTCAGGAGTAATATCCATCTTTTCAGCGATTTCTTCAATCGTTGCATC
TCGTCCTAATTCTTGAACTAATTGACGTTGAATACGAATCATTTTATTAATTGTTTCAAC
CATATGAACAGGAATACGGATTGTTCTTGCCTGATCGGCTACCGCTCTAGTAATCGCTTG
GCGAATCCACCATGTTGCATATGTTGAAAATTTAAATCCTTTTTCATAATCAAATTTATC
CACAGCACGCATAAGACCCATATTACCTTCTTGAATAAGGTCTAGAAATAATAAACCACG
TTGTGTATATTTTTTAGCAATACTTACAACTAAACGGTAGTTAGCTTCAACTAATTTTTC
TTTAGCATAATGACCTTTTCTAATTGTATCTTCTAATGATTGCACATCTTCATCTGATAA
ATCTACTTCGCCATTTCTTACTGCCTCAAGTTGTTCTAAAGCTACACGACCTTGGTAAAC
CATGATTGCATATTTTCTTTCATCATCAATATTTAGTAATGGAATTTGACCAATTTCTTT
TAAGTACATACGTACAGGATCATCTACTTTAATACTTGAAGGAATCGCGTCAATATTTAG
AAGTTCTTCTTCTTCAACTTCTTCAACTTCTAAACTAGATAGTGAAAACTCATCTGGTTC
ATCTTCAACTTCTAACTCTACTTCAATGTCTTCATCATCAAAAAGGGGATTTTCTTGAAC
ATCAACCTCTTCTTCTTCATCATCCAGTAATACATCAATTTCTAGATCTGATAATTCTTT
TTCTATTTCATAATATTCAGGGGAATTTAAATCAGCAAACTCAATTAAATCGGATTGAAC
TAATGACTTTTTCTTACCTGCTTTTTTAACTAACTTCTTTATGATATCTTTTTGACTCAT

>protein sequence
MSQKDIIKKLVKKAGKKKSLVQSDLIEFADLNSPEYYEIEKELSDLEIDVLLDDEEEEVD
VQENPLFDDEDIEVELEVEDEPDEFSLSSLEVEEVEEEELLNIDAIPSSIKVDDPVRMYL
KEIGQIPLLNIDDERKYAIMVYQGRVALEQLEAVRNGEVDLSDEDVQSLEDTIRKGHYAK
EKLVEANYRLVVSIAKKYTQRGLLFLDLIQEGNMGLMRAVDKFDYEKGFKFSTYATWWIR
QAITRAVADQARTIRIPVHMVETINKMIRIQRQLVQELGRDATIEEIAEKMDITPEKVQN
IQRIAKEPISLEAPVGEEEDSSLGDFISDPNALNPHDYMMQEMVKKTLDEVLETLTDREE
KVLRLRYGLLDGKTHTLEEVGREFGVTRERIRQIEAKALRKLRSPQRQNKLKEFYITRKW