ACL_RS04680

Uniprot: A9NGS6
Description: glycine--tRNA ligase
EC number: 6.1.1.14
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00015999}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the attachment of glycine to tRNA(Gly). {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00345537}.
Pathway:
Active site:
Binding site: BINDING 97 97 Substrate. {ECO:0000256|HAMAP-Rule:MF_00253}.; BINDING 167 167 Substrate. {ECO:0000256|HAMAP-Rule:MF_00253}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 199 201 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 209 214 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 283 284 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 327 330 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.
Site:
Gene names (primary): glyS
Gene names (synonym): glyQS
Mass: 53,149
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00006558}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; glycine-tRNA ligase activity [GO:0004820]; glycyl-tRNA aminoacylation [GO:0006426]
Gene ontology IDs: GO:0004820; GO:0005524; GO:0005737; GO:0006426
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00541124}.
Protein families: Class-II aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]: DOMAIN 6 362 AA_TRNA_LIGASE_II. {ECO:0000259|PROSITE:PS50862}.
Motif:
Region: REGION 214 218 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00253}.; REGION 323 327 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00253}.
EMBL: CP000896
ProteinModelPortal: A9NGS6
MEROPS:
EnsemblBacteria KO: ABX81556
UniPathway: K01880
CDD:
Gene3D: cd00774
HAMAP: 3.40.50.800
InterPro: MF_00253_B
PANTHER: IPR002314;IPR006195;IPR004154;IPR027031;IPR022961;IPR033731;IPR002315
PIRSF: PTHR10745
PRINTS:
PROSITE: PR01043
Pfam: PS50862
ProDom: PF03129;PF00587
SMART:
SUPFAM:
TIGRFAMs: SSF52954

972406-973777(-)

>nucleotide sequence
TTAAAATTTAGTTGCTTGTTGAATAAATTGTTCGATGTCTTTAATTGCAATACGAGATTG
CTCCATAGAATCACGGTGTCTAATAGTTACTGTATCATCATCCATTGTTTGATGATCAAC
TGTAATACAAAATGGCGTTCCAATCGCGTCTTGACGACGATATCTTTTACCAATATTTTG
TGTTTCATCGTAAACAACATCTAGGTTTTCACTTAACATTTCATGAATTTCTCTAGCTTT
TTGACTATGTTCTTTACGTACAAGTGGTAAGATAGCAGCTTTATATGGTGCAAGTTTTGG
ATGTAGTTTTAAGACTTGTCTTACATCACCATTTTCTAAAGTTTCTTCTTCTAAACCATT
TAATAAGAATACTAATAATAGTCTTTCAACACCAACGGATGGTTCTACGACATAAGGAAT
GTATCTTTCATTGGTTTCAGGATCTAGATAAGACATATCTTCCTTACTTAAGTCTTGATG
ACTCTTAAGGTCAAAGTTTGTACGACTTGCAATACCCCAAAGTTCATCAAAACCCCACGG
ATATTTAAATTCGATATCTGTTGTAGCATTTGAATAGTGACTTAGTGCTTCCTTAGCATG
TGGTTCAGCACGCAAACTTTCTTCTTTTACACCAAGGGATGTTAAGAATTTAAGCATTCT
GTCTAACCAGTAATCAAACCACTGGATTTCAGTACCTGGTTTACAGAAAAACTCTAATTC
CATTTGTTCAAATTCTCTCGTTCTAAATGTGAAGTTACCTGGTGTAATTTCATTTCTAAA
ACTCTTACCAATTTGAGCAACACCAAATGGCACTTTAAGACGTTGTGCACGTTGGATATT
TTTAAAGTTAATAAAGATACCTTGAGCTGTTTCTGGACGAAGATACACTTCATTTGCAGT
TTCTTCTACAACCCCTTGGAAAGTTTTAAACATCATATTAAACTTTCTAATTGGTAACCA
ATCACTTGTTTTAGACACTGGGTCTTTGATTTTATGATCCATAATATATTGATACATCTT
CTCATCACTCCAGCCATCTGGATTTTGAGTCGGATCGTGCTCTAAAATTAATTTGTCAGC
ACGGTATCTTTGATTAGATGTTTTATTTTCAATTAATGGGTCTGAAAAACCACTTAAGTG
ACCACTAGCTTGCCATACTAATGGGTTTAATAGAATTGAAGAATCTAGTTGAACCACATT
AGGCGCTTGACGAATAAACTTTTGCGTCCAAGCTTCTTTAATATTTTTCTTTAATAATGA
ACCTAGAGGACCGTAATCCCAAGTATTTGCAAGCCCACCATAGATTTCAGAGCCTTGAAA
GATGTAACCTGTTTGTTTTGCGTATTGTACTAAATACTCTAATGTAACCAT

>protein sequence
MVTLEYLVQYAKQTGYIFQGSEIYGGLANTWDYGPLGSLLKKNIKEAWTQKFIRQAPNVV
QLDSSILLNPLVWQASGHLSGFSDPLIENKTSNQRYRADKLILEHDPTQNPDGWSDEKMY
QYIMDHKIKDPVSKTSDWLPIRKFNMMFKTFQGVVEETANEVYLRPETAQGIFINFKNIQ
RAQRLKVPFGVAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTEIQWFDYWLDRML
KFLTSLGVKEESLRAEPHAKEALSHYSNATTDIEFKYPWGFDELWGIASRTNFDLKSHQD
LSKEDMSYLDPETNERYIPYVVEPSVGVERLLLVFLLNGLEEETLENGDVRQVLKLHPKL
APYKAAILPLVRKEHSQKAREIHEMLSENLDVVYDETQNIGKRYRRQDAIGTPFCITVDH
QTMDDDTVTIRHRDSMEQSRIAIKDIEQFIQQATKF