ACL_RS05715

Uniprot: A9NHC4
Description: UMP kinase
EC number: 2.7.4.22
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + UMP = ADP + UDP. {ECO:0000256|HAMAP-Rule:MF_01220, ECO:0000256|SAAS:SAAS00197246}.
Cofactor:
Enzyme regulation: ENZYME REGULATION: Inhibited by UTP. {ECO:0000256|HAMAP-Rule:MF_01220}.
Function [CC]: FUNCTION: Catalyzes the reversible phosphorylation of UMP to UDP. {ECO:0000256|HAMAP-Rule:MF_01220, ECO:0000256|SAAS:SAAS00558287}.
Pathway: PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1. {ECO:0000256|HAMAP-Rule:MF_01220, ECO:0000256|SAAS:SAAS00197268}.
Active site:
Binding site: BINDING 51 51 ATP; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_01220}.; BINDING 55 55 ATP. {ECO:0000256|HAMAP-Rule:MF_01220}.; BINDING 70 70 UMP. {ECO:0000256|HAMAP-Rule:MF_01220}.; BINDING 159 159 ATP. {ECO:0000256|HAMAP-Rule:MF_01220}.; BINDING 165 165 ATP; via amide nitrogen and carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_01220}.; BINDING 168 168 ATP. {ECO:0000256|HAMAP-Rule:MF_01220}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 8 11 ATP. {ECO:0000256|HAMAP-Rule:MF_01220}.; NP_BIND 131 138 UMP. {ECO:0000256|HAMAP-Rule:MF_01220}.
Site:
Gene names (primary): pyrH
Gene names (synonym):
Mass: 25,845
Subunit structure [CC]: SUBUNIT: Homohexamer. {ECO:0000256|HAMAP-Rule:MF_01220, ECO:0000256|SAAS:SAAS00558304}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; UMP kinase activity [GO:0033862]; 'de novo' CTP biosynthetic process [GO:0044210]
Gene ontology IDs: GO:0005524; GO:0005737; GO:0033862; GO:0044210
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the UMP kinase family. {ECO:0000256|HAMAP-Rule:MF_01220, ECO:0000256|SAAS:SAAS00558321}.
Protein families: UMP kinase family
Coiled coil:
Domain [FT]: DOMAIN 4 212 AA_kinase. {ECO:0000259|Pfam:PF00696}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NHC4
MEROPS:
EnsemblBacteria KO: ABX81754
UniPathway: K09903
CDD:
Gene3D:
HAMAP: 3.40.1160.10
InterPro: MF_01220_B
PANTHER: IPR001048;IPR011817;IPR015963
PIRSF: PTHR21499:SF23
PRINTS: PIRSF005650
PROSITE:
Pfam:
ProDom: PF00696
SMART:
SUPFAM:
TIGRFAMs: SSF53633

1211063-1211783(-)

>nucleotide sequence
TCATGAGTTTGCTCCGTTTCTTACAACTGTACCGATGTCTTCTCCTTTAGCTGCCTTTAG
AATGTTGCCAGGTTTATTCATATCAAATACAAGTATTTCAATATGGTTGTCCTTGCAAAG
ACTTGCTGCTGTAGAATCCATGATTTGTAAATTTTTTTCTAGGACTTGTTGATGTGATAC
TTCTCTTAAAAGTACAGCATCACTAAATTTATTAGGGTCTTTATCATAGACACCATCTAC
ACCATTTTTGGCCATTAAGATAACATCGCTATCTAATTCAGCTGCACGGAGTGCTGCTGT
TGTATCAGTTGAAAAATATGGGGATCCTGTGCCACCTGCTAATATGACTACACGCCCTTT
TTCAAAGTGTCTTAATGCACGTCTTCGAATATAAGGTTCAGCTACAGCTGCAATTTGTAG
TGCTGTCATTACACGGCTTGGGCTTTCAATAGCTTCAAGTGCGTCTTGTAGGGCTAAACT
GTTCATGATCGTACCTAGCATACCCATGTAGTCTGCTTGGGCACGGTCCATACCAAGTTC
TTCACCGGTCTTACCACGCCATAAATTACCTGCACCGACAACGACTGCAACTTCTATGCC
AGCGTTACGGACAGTTTTAATTTCTTGAGCAATCTTTTTAACTGTTTTAGGATCAATGCC
ATATTGGCCATCTCCTTTTAAAGCTTCTCCAGATATTTTTAAAATGATTCGACGATACAT

>protein sequence
MYRRIILKISGEALKGDGQYGIDPKTVKKIAQEIKTVRNAGIEVAVVVGAGNLWRGKTGE
ELGMDRAQADYMGMLGTIMNSLALQDALEAIESPSRVMTALQIAAVAEPYIRRRALRHFE
KGRVVILAGGTGSPYFSTDTTAALRAAELDSDVILMAKNGVDGVYDKDPNKFSDAVLLRE
VSHQQVLEKNLQIMDSTAASLCKDNHIEILVFDMNKPGNILKAAKGEDIGTVVRNGANSW