ACL_RS05765

Uniprot: A9NHD4
Description: uracil-DNA glycosylase
EC number: 3.2.2.27
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. {ECO:0000256|HAMAP-Rule:MF_00148, ECO:0000256|RuleBase:RU003780, ECO:0000256|SAAS:SAAS00354267}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. {ECO:0000256|HAMAP-Rule:MF_00148, ECO:0000256|RuleBase:RU003780}.
Pathway:
Active site: ACT_SITE 64 64 Proton acceptor. {ECO:0000256|HAMAP-Rule:MF_00148}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): ung
Gene names (synonym):
Mass: 25,642
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; uracil DNA N-glycosylase activity [GO:0004844]; base-excision repair [GO:0006284]
Gene ontology IDs: GO:0004844; GO:0005737; GO:0006284
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the uracil-DNA glycosylase family. {ECO:0000256|HAMAP-Rule:MF_00148, ECO:0000256|RuleBase:RU003780, ECO:0000256|SAAS:SAAS00547419}.
Protein families: Uracil-DNA glycosylase family
Coiled coil:
Domain [FT]: DOMAIN 49 208 UDG. {ECO:0000259|SMART:SM00986}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NHD4
MEROPS:
EnsemblBacteria KO: ABX81764
UniPathway: K03648
CDD:
Gene3D: cd10027
HAMAP: 3.40.470.10
InterPro: MF_00148
PANTHER: IPR002043;IPR018085;IPR005122
PIRSF: PTHR11264
PRINTS:
PROSITE:
Pfam: PS00130
ProDom: PF03167
SMART:
SUPFAM: SM00986
TIGRFAMs: SSF52141

1219129-1219795(-)

>nucleotide sequence
TTATTTAATATGCCAGTCAACTGGATTGACATGATGCATCATTAAATAATGGTTTGCTTT
TGAAAAAGGTTTAGAACCAAAGAATCCTAATCTTGCTGAAAAAGGTGATGGGTGTACACT
TTCTATCACCATGTGTTTGGGGTTCGTTAAAAGTGGTTTAATGTCTTTTGCTTGATTCCC
CCAAAGGATAAAGACAATCGGTTGATTTAATAAATTGATATGTCTAATCATTTGGTGTGT
AAAGATTTCCCACCCTTTATTCTTATGAGAAAGTGGTTTAGATGCTTCAACAGTTAAGAC
CGTATTAAGCAACAAAACACCCTGCCTAGCCCAGGATGTTAAATCACCTTTTGTTGATAT
ATCGATATTTAAATCATCTTTTAATTCTTTATATATGTTTTTTAAGCTTGGTGGTAGTTT
TTCATTTAATGTTGAAAAACTTAAACCATGCGCTTGGTTTGGTCCATGATATGGATCTTG
ACCAAGGATAACAACTTTAAGATTATTTACAGAAGTATATAAAAACGCATTAAACCAATC
CTTTTTTGGTGGATATATCGTTTCAGTTAAAGAACGCTCATCCAAAAAAGACATGAGATT
TTTAAAATACGGCTTTATTAGTTCTTCATTCATCCAGTTTGTCCACATTGGTATTTGTAT
CTCCAT

>protein sequence
MEIQIPMWTNWMNEELIKPYFKNLMSFLDERSLTETIYPPKKDWFNAFLYTSVNNLKVVI
LGQDPYHGPNQAHGLSFSTLNEKLPPSLKNIYKELKDDLNIDISTKGDLTSWARQGVLLL
NTVLTVEASKPLSHKNKGWEIFTHQMIRHINLLNQPIVFILWGNQAKDIKPLLTNPKHMV
IESVHPSPFSARLGFFGSKPFSKANHYLMMHHVNPVDWHIK