ACL_RS05850

Uniprot: A9NHF1
Description: tryptophan--tRNA ligase
EC number: 6.1.1.2
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). {ECO:0000256|HAMAP-Rule:MF_00140, ECO:0000256|SAAS:SAAS00009937}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site: BINDING 199 199 ATP. {ECO:0000256|HAMAP-Rule:MF_00140}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): trpS
Gene names (synonym):
Mass: 37,723
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00140}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; tryptophan-tRNA ligase activity [GO:0004830]; tryptophanyl-tRNA aminoacylation [GO:0006436]
Gene ontology IDs: GO:0004830; GO:0005524; GO:0005737; GO:0006436
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00140, ECO:0000256|RuleBase:RU363036, ECO:0000256|SAAS:SAAS00538757}.
Protein families: Class-I aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]:
Motif: MOTIF 10 18 "HIGH" region. {ECO:0000256|HAMAP-Rule:MF_00140}.; MOTIF 196 200 "KMSKS" region. {ECO:0000256|HAMAP-Rule:MF_00140}.
Region:
EMBL: CP000896
ProteinModelPortal: A9NHF1
MEROPS:
EnsemblBacteria KO: ABX81781
UniPathway: K01867
CDD:
Gene3D:
HAMAP: 3.40.50.620
InterPro: MF_00140_B
PANTHER: IPR001412;IPR002305;IPR014729;IPR002306;IPR024109
PIRSF: PTHR10055
PRINTS:
PROSITE: PR01039
Pfam: PS00178
ProDom: PF00579
SMART:
SUPFAM:
TIGRFAMs:

1235583-1236588(+)

>nucleotide sequence
ATGAAAAGACTTGTATCAGGCATTAAACCAACCGGTGAGTTAACACTTGGTAACTACATT
GGTGCAATCAAACAATTTGTTAAACTACAAAATGAATTACCAGATACCGAATTCTTTATT
TTTATTGCTGACCTACATGCAATAACAACCCCACAAGATAAGCAATTATTAAAAAAGAGA
GTACGCGATATTGCAGCATACTATATCGCATGTGGACTGGATCCAAACAGAGTGAACTTA
TTTGTTCAATCTGAGGTGATGGAACACGCTATGCTTGGTTACATTATGGAATCTACTGCT
TATATTAATGAAATGGAGCGTATGATTCAGTTTAAAGAAAAGCGTACCTCACAAACTGAA
GGCGTTCGAACTTCCCTTTTAACTTATCCAGCACTCATGGCTGCAGATATTTTACTATAT
GATGCAAGTATCGTACCAGTTGGGGAAGACCAAACTCAGCACTTAGAACTTACAAGAACA
CTAGCTCAAAGATTTAATCACTTATATGGTGATACATTCGTCATACCTGAAGGTCATGTT
ACTAAAGTTGGTGCTAAGATTAAAGACCTTCAAGAACCAACCAAAAAAATGAGTAAGTCT
GAGGGCTTATCTCAAAAATCTTATATTTTATTAGAAGATAATTTAAATCAAATTAAAAAT
AAGATCAAGTCTGCTGTAACAGATTCAGATACTAAGATATATTTTGATGAAGTCAATAAA
CCAGGTATCTCTAACTTGCTTACAATTTATTCAGCAATTACAGATACACCAATGAGTGAT
ATCGTGGATAAATACAAAGATGAAACATCTTATGCCGGCTTTAAATCTGATTTAGCTGAA
ATCGTTGCAGAACATATTGGTCAAATCCAAGAAAATTATAAAACTTTAATGGCTTCAAAT
ACCTTAGACGATATATTAGATACGGGTGCTCAAAAAGCGTCCATGTATGCTCAAAGAAAA
ATTTTGAAAATCCTCTCGAAAATGGGCTTGAAAAGAAAAAAGTGA

>protein sequence
MKRLVSGIKPTGELTLGNYIGAIKQFVKLQNELPDTEFFIFIADLHAITTPQDKQLLKKR
VRDIAAYYIACGLDPNRVNLFVQSEVMEHAMLGYIMESTAYINEMERMIQFKEKRTSQTE
GVRTSLLTYPALMAADILLYDASIVPVGEDQTQHLELTRTLAQRFNHLYGDTFVIPEGHV
TKVGAKIKDLQEPTKKMSKSEGLSQKSYILLEDNLNQIKNKIKSAVTDSDTKIYFDEVNK
PGISNLLTIYSAITDTPMSDIVDKYKDETSYAGFKSDLAEIVAEHIGQIQENYKTLMASN
TLDDILDTGAQKASMYAQRKILKILSKMGLKRKKW