ACL_RS05865

Uniprot: A9NHF4
Description: tyrosine--tRNA ligase
EC number: 6.1.1.1
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). {ECO:0000255|HAMAP-Rule:MF_02006}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). {ECO:0000255|HAMAP-Rule:MF_02006}.
Pathway:
Active site:
Binding site: BINDING 34 34 Tyrosine. {ECO:0000255|HAMAP-Rule:MF_02006}.; BINDING 163 163 Tyrosine. {ECO:0000255|HAMAP-Rule:MF_02006}.; BINDING 167 167 Tyrosine. {ECO:0000255|HAMAP-Rule:MF_02006}.; BINDING 227 227 ATP. {ECO:0000255|HAMAP-Rule:MF_02006}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): tyrS
Gene names (synonym):
Mass: 46,427
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_02006}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; RNA binding [GO:0003723]; tyrosine-tRNA ligase activity [GO:0004831]; tyrosyl-tRNA aminoacylation [GO:0006437]
Gene ontology IDs: GO:0003723; GO:0004831; GO:0005524; GO:0005737; GO:0006437
Chain: CHAIN 1 410 Tyrosine--tRNA ligase. /FTId=PRO_1000088572.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_02006}.; SIMILARITY: Contains 1 S4 RNA-binding domain. {ECO:0000255|HAMAP-Rule:MF_02006}.
Protein families: Class-I aminoacyl-tRNA synthetase family, TyrS type 1 subfamily
Coiled coil:
Domain [FT]: DOMAIN 344 410 S4 RNA-binding. {ECO:0000255|HAMAP-Rule:MF_02006}.
Motif: MOTIF 39 48 "HIGH" region.; MOTIF 224 228 "KMSKS" region.
Region:
EMBL: CP000896
ProteinModelPortal: A9NHF4
MEROPS:
EnsemblBacteria KO: ABX81784
UniPathway: K01866
CDD:
Gene3D:
HAMAP: 3.10.290.10;3.40.50.620
InterPro: MF_02006
PANTHER: IPR001412;IPR002305;IPR014729;IPR002942;IPR002307;IPR024088;IPR024107
PIRSF: PTHR11766
PRINTS:
PROSITE: PR01040
Pfam: PS00178;PS50889
ProDom: PF01479;PF00579
SMART:
SUPFAM:
TIGRFAMs:

1239230-1240463(-)

>nucleotide sequence
TTAAAATACGATGAGTGCGTATTTTTTCTTCCCTCTTCTAATGATTGCATATGTATCAAA
TGCTTTTGATGTTTCTCCTACATTATACATGGAATCTGTTATCTTTTCACCTTGAATACT
TATAGCACCATTTGAAATAAACTCTCTTGCTTCTCTGTTAGAGGAAGCAAGTTTTGATTT
TACTACAAGCTCAATTAAACTTTCACCATTAAAACGCACCACGTCAATAACGCTTTCTAG
TGTCTTATAAGCTGATTTTGTTAAACCATTAAACTCACCACTAAATAATGCTTCTGTTAC
TTGGAGTGCAGTTTCTAATTCTTCTTTACCATGTACTAATTCTACAACCGCTTTAGCTAA
TACTTTTTGAGCGTGGCGTAGCTCTGGTTTTTCACGGTGTGTTTCTAAAATGGCTTCAAT
CTCATCTTGTTCAATTAATGTTAGAAGTTTTAAGAATTGTTCAATATCTGTATCACTACT
ATTTAAAAAGTATTGGTATAACTCATAAGGTGATGTAAGTTCTTTATCTAGCCATAATGC
ACCAGATTCTGATTTACCAAATTTTGTACCATCCGCTTTTAATAATAGTGGGCTAGATAA
TCCAACTGCATCATTTTTACCTAATACACGGCGAATCAGTTCTAATCCAGATGTTAAGTT
ACCCCATTGGTCTGATCCACCAAATTGAATTTTAACATCACGGTTTTGGTATAGATTTAA
CCAGTCAATGGACTGTAAAATCATATAAGAAAACTCTGTATAAGAAATACCTGATTCTAA
TCTTTTTTGAACCGTATCTTTATGTAACATATAAGATACACTAAAATGTTTTCCATAATC
TCTTAAGAAACTAATTAAATCGATTTTAGATAACCATTCATAGTTATTTACAAACTCTAC
ATTACCTTTAATAAGTCCTTTGATTTGGTTTTCAATTTTCTGAGCATTTTCAAGTGATTT
TTCAAGTGTTAATAAAGAACGTTCACTTGTTTCTCTTGGATCTCCAATCAAACCTGTACC
ACCACCAATTAATACAACCGGTGTATGTCCATGTTTTTGTAGGTATAACATACGTACAAT
TTGGACTAAGTGTCCGACTGTTAAAGATTCACCTGTTGGGTCATAACCCACATAAAATTT
AATTTTTTGTTCATTTAATAATTGTTTAGCAAGTTCAAGATCACTAACATCTTTGACCAT
GCCTCTATATTCTAGTTGTTTAAATAAGTCCAT

>protein sequence
MDLFKQLEYRGMVKDVSDLELAKQLLNEQKIKFYVGYDPTGESLTVGHLVQIVRMLYLQK
HGHTPVVLIGGGTGLIGDPRETSERSLLTLEKSLENAQKIENQIKGLIKGNVEFVNNYEW
LSKIDLISFLRDYGKHFSVSYMLHKDTVQKRLESGISYTEFSYMILQSIDWLNLYQNRDV
KIQFGGSDQWGNLTSGLELIRRVLGKNDAVGLSSPLLLKADGTKFGKSESGALWLDKELT
SPYELYQYFLNSSDTDIEQFLKLLTLIEQDEIEAILETHREKPELRHAQKVLAKAVVELV
HGKEELETALQVTEALFSGEFNGLTKSAYKTLESVIDVVRFNGESLIELVVKSKLASSNR
EAREFISNGAISIQGEKITDSMYNVGETSKAFDTYAIIRRGKKKYALIVF