ACL_RS05880

Uniprot: A9NHF7
Description: tRNA (guanosine(37)-N1)-methyltransferase TrmD
EC number: 2.1.1.228
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. {ECO:0000255|HAMAP-Rule:MF_00605}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Specifically methylates guanosine-37 in various tRNAs. {ECO:0000255|HAMAP-Rule:MF_00605}.
Pathway:
Active site:
Binding site: BINDING 111 111 S-adenosyl-L-methionine; via amide nitrogen. {ECO:0000255|HAMAP-Rule:MF_00605}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): trmD
Gene names (synonym):
Mass: 27,983
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00605}.
Gene ontology (GO): cytoplasm [GO:0005737]; tRNA (guanine(37)-N(1))-methyltransferase activity [GO:0052906]
Gene ontology IDs: GO:0005737; GO:0052906
Chain: CHAIN 1 243 tRNA (guanine-N(1)-)-methyltransferase. /FTId=PRO_1000082505.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA methyltransferase TrmD family. {ECO:0000255|HAMAP-Rule:MF_00605}.
Protein families: RNA methyltransferase TrmD family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 130 135 S-adenosyl-L-methionine binding. {ECO:0000255|HAMAP-Rule:MF_00605}.
EMBL: CP000896
ProteinModelPortal: A9NHF7
MEROPS:
EnsemblBacteria KO: ABX81787
UniPathway: K00554
CDD:
Gene3D:
HAMAP: 1.10.1270.20;3.40.1280.10
InterPro: MF_00605
PANTHER: IPR029028;IPR002649;IPR023148;IPR029026;IPR016009
PIRSF:
PRINTS: PIRSF000386
PROSITE:
Pfam:
ProDom: PF01746
SMART:
SUPFAM:
TIGRFAMs: SSF75217

1242328-1243060(-)

>nucleotide sequence
CTATTGGGATTCAAGATTTTTTTTTATTTTACTTAATATCTTACTTTCTTCCTTGGTAAG
ATCTCTATTCACTAATAAATCAGGTCTTTTGAGATATGTTTTCTCTAAACTCTTCTCTAA
GCGCCATAATCTAATTTTTTCATGATGTCCAGATTTTAATATATCTGGAACGCTCATGCC
TAAATATTCATCAGGTTTAGTATATTGTGGGTATTTAAGTAACCCTTGTTGATGGGTATC
TTCCATGTAAGATGCTTCATTAATCACACCAGGAATTAACCTTGTAATACTGTCTGTAAT
CACCATTGCAGGTATCTCACCGCCAGTTAATACATAATCACCAATACTTATTTCTTCATC
GATTAAGTTTTGAATACGATCATCAATACCTTCATAATGCCCACAAATTAAAATTAAGTG
TTTAATTTCATCACGCCACTTGGTTGCTACTTGTTGATTGAAGACATTACCTTGTGGTGA
TAATAAAACTGTATACGTATCTTTAGTTTTTAATTTGTTAATAAGTTTATGAAAAGGTGG
ATAGATCATTAACATACCCGCACCACCACCATAGGGTGTATCATCAATTTTTCCACCTTT
TAAATCGGTATACAATCTTAAGTCGTGGATATTGATTTCCACACGCTTATCTTCGATTGC
ATGCTTGATTTTAGATGTTGTTAAAAATTGATCAAAGAAGTTTGGGAAGATTGTAATAAT
ATCAATAATCAT

>protein sequence
MIIDIITIFPNFFDQFLTTSKIKHAIEDKRVEINIHDLRLYTDLKGGKIDDTPYGGGAGM
LMIYPPFHKLINKLKTKDTYTVLLSPQGNVFNQQVATKWRDEIKHLILICGHYEGIDDRI
QNLIDEEISIGDYVLTGGEIPAMVITDSITRLIPGVINEASYMEDTHQQGLLKYPQYTKP
DEYLGMSVPDILKSGHHEKIRLWRLEKSLEKTYLKRPDLLVNRDLTKEESKILSKIKKNL
ESQ