ACL_RS05930

Uniprot: A9NHG7
Description: tRNA sulfurtransferase ThiI
EC number: 2.8.1.4
Annotation score: 4 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. {ECO:0000255|HAMAP-Rule:MF_00021}.; CATALYTIC ACTIVITY: [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. {ECO:0000255|HAMAP-Rule:MF_00021}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. {ECO:0000255|HAMAP-Rule:MF_00021}.
Pathway: PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00021}.
Active site:
Binding site: BINDING 265 265 ATP. {ECO:0000255|HAMAP-Rule:MF_00021}.; BINDING 287 287 ATP; via amide nitrogen. {ECO:0000255|HAMAP-Rule:MF_00021}.; BINDING 296 296 ATP. {ECO:0000255|HAMAP-Rule:MF_00021}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 181 182 ATP. {ECO:0000255|HAMAP-Rule:MF_00021}.; NP_BIND 206 207 ATP. {ECO:0000255|HAMAP-Rule:MF_00021}.
Site:
Gene names (primary): thiI
Gene names (synonym):
Mass: 46,583
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; sulfurtransferase activity [GO:0016783]; tRNA adenylyltransferase activity [GO:0004810]; tRNA binding [GO:0000049]; thiamine biosynthetic process [GO:0009228]; thiamine diphosphate biosynthetic process [GO:0009229]; tRNA thio-modification [GO:0034227]
Gene ontology IDs: GO:0000049; GO:0004810; GO:0005524; GO:0005737; GO:0009228; GO:0009229; GO:0016783; GO:0034227
Chain: CHAIN 1 412 Probable tRNA sulfurtransferase. /FTId=PRO_1000074196.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the ThiI family. {ECO:0000255|HAMAP-Rule:MF_00021}.; SIMILARITY: Contains 1 THUMP domain. {ECO:0000255|HAMAP-Rule:MF_00021}.
Protein families: ThiI family
Coiled coil:
Domain [FT]: DOMAIN 58 163 THUMP. {ECO:0000255|HAMAP-Rule:MF_00021}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NHG7
MEROPS:
EnsemblBacteria KO: ABX81797
UniPathway: K03151
CDD:
Gene3D:
HAMAP: 3.40.50.620
InterPro: MF_00021
PANTHER: IPR014729;IPR020536;IPR004114;IPR003720
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51165
ProDom: PF02568;PF02926
SMART:
SUPFAM: SM00981
TIGRFAMs:

1249243-1250482(-)

>nucleotide sequence
CTACTCCTGTGTATATGAACTTAATGCATCTTTCACATCAAAGCCGTGTAATGCGATTTC
AAAATCAGTGGTAGGGGTAATAATCATTGTTTGAACATTCTTAAGTGCATCTTCTAAGAG
TTCTTTAAACTCAAATCTAGATTCTTCTTTCAATGCTTGGTCAATGGTTGGTTTAGTAAC
TGGGTTTCTTGGTACATAAATTGAGCAGCAATCATTAAACGGTAAAATAGATATATCATA
TGTATCTATTTTCTTAGATATATTAATAATATCATTTTTGTCATAAGTAATAACCGGTCT
TAAAATAGGTATAGAGGTTACATTTTCAATAACTTTAATACTACTTAAGGTTTGACTTGC
TACCTGGCCAACGGATTCACCATTAATTAAGGTATCTAAACCATGTTGATTTGCATAGAT
TTCACCTAATCTGTAGAACATACGACGCATGATCGTAATAATGTACGGATCTGCTACATA
ACTTAGTATCGCTTCATGTATTTTAGTAAACGGTACAAGATGTAGTTTAATCTTATTATA
TGGTGTATAGTATGCTAATTTTTTTGCAATTTCTATAACCTTTTGAACAGATTCTAATGG
TGTAAGTGGTGTGGATTCAAAGTGGAATAATTCCACATCAATGCCTTGCTTCATCATAAG
ATGTGCCGCCACTGGGGAATCAATACCACCAGATAACATCACTAAGCCTTTACCACCTAA
ACCTATTGGAAAACCACCTAGTGCAGGTATTTTACCAACATAGATATAAGTGCCTTCGCT
TCTTAGTTCAATATCTAAAACTGTTTCAGGATTTCTCACTTCTACTTTTAACCCGTCAAA
TGATGGAAGTACGAGTGATGCCACTTTTTGTGAGATTTCTAAAGATTTTAGTGGATAGTT
TTTATCCGTTCTCTTCGTCTCTATTTTAAATGTTGTAGGAAGTTTAACTTCCTTTTGAAT
CACTTCTTTTGCAAGCTGTGCAATAGATTCAATATCTTTTGTTGTTTTGTAGATGTAACT
AAATGAGTGTATACCGGATACATATCCTAGTTGTTTAATCACTTCATCACTATCTACTTC
ATTAAAATGAACATATATACGATCATGTTGGAAAGTATACTCTACATTTACGTTTTTTAA
CTTACTTCTTATATTTTTTTTAATCTGGCCGATGAATTTAGGTTTGTTCTTACCTTTTAG
TACTAAATCACCAAAGCGAATTAAAATTGCCTTTTCCAT

>protein sequence
MEKAILIRFGDLVLKGKNKPKFIGQIKKNIRSKLKNVNVEYTFQHDRIYVHFNEVDSDEV
IKQLGYVSGIHSFSYIYKTTKDIESIAQLAKEVIQKEVKLPTTFKIETKRTDKNYPLKSL
EISQKVASLVLPSFDGLKVEVRNPETVLDIELRSEGTYIYVGKIPALGGFPIGLGGKGLV
MLSGGIDSPVAAHLMMKQGIDVELFHFESTPLTPLESVQKVIEIAKKLAYYTPYNKIKLH
LVPFTKIHEAILSYVADPYIITIMRRMFYRLGEIYANQHGLDTLINGESVGQVASQTLSS
IKVIENVTSIPILRPVITYDKNDIINISKKIDTYDISILPFNDCCSIYVPRNPVTKPTID
QALKEESRFEFKELLEDALKNVQTMIITPTTDFEIALHGFDVKDALSSYTQE