ACL_RS05970


  Uniprot: A9NHH5
Description: triose-phosphate isomerase
EC number: 5.3.1.1
Annotation score: 3 out of 5
Miscellaneous [CC]: 
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate = glycerone phosphate. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00525314}.
Cofactor: 
Enzyme regulation: 
Function [CC]: FUNCTION: Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P). {ECO:0000256|HAMAP-Rule:MF_00147}.
Pathway: PATHWAY: Carbohydrate biosynthesis; gluconeogenesis. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00498179}.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00498183}.
Active site: ACT_SITE 96 96 Electrophile. {ECO:0000256|HAMAP-Rule:MF_00147}.; ACT_SITE 168 168 Proton acceptor. {ECO:0000256|HAMAP-Rule:MF_00147}.
Binding site: BINDING 174 174 Substrate; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00147}.; BINDING 214 214 Substrate. {ECO:0000256|HAMAP-Rule:MF_00147}.
Calcium binding: 
DNA binding: 
Metal binding: 
Nucleotide binding: 
Site: 
Gene names (primary): tpiA
Gene names (synonym): 
Mass: 27,803
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00498221}.
Gene ontology (GO): cytoplasm [GO:0005737]; triose-phosphate isomerase activity [GO:0004807]; gluconeogenesis [GO:0006094]; glycolytic process [GO:0006096]; pentose-phosphate shunt [GO:0006098]
Gene ontology IDs: GO:0004807; GO:0005737; GO:0006094; GO:0006096; GO:0006098
Chain: 
Signal peptide: 
Domain [CC]: 
Sequence similarities: SIMILARITY: Belongs to the triosephosphate isomerase family. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00541575}.
Protein families: Triosephosphate isomerase family
Coiled coil: 
Domain [FT]: 
Motif: 
Region: REGION 11 13 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00147}.; REGION 235 236 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00147}.
EMBL: CP000896
ProteinModelPortal: A9NHH5
MEROPS: 
EnsemblBacteria KO: ABX81805
UniPathway: K01803
CDD: 
Gene3D: cd00311
HAMAP: 3.20.20.70
InterPro: MF_00147_B
PANTHER: IPR013785;IPR022896;IPR000652;IPR020861
PIRSF: PTHR21139
PRINTS: 
PROSITE: 
Pfam: PS00171;PS51440
ProDom: PF00121
SMART: 
SUPFAM: 
TIGRFAMs: SSF51351
1256040-1256805(-)

>nucleotide sequence
TTATTTGTTTTTTGCTGCTTTTGTAAATGTTAAGAAGTTTTTATGATCTAATGCTGCGCC
ACCGATTAAAGCACCATCGATGTCTGATTCTTGTAATAGACTTTCGATGTTATCTGGTTT
AACAGAACCACCATATAAGATACGTACTTGAGCTGCAACTTCAGCACCATATAGGTCACT
TAGTTTAGCACGTACATCTTTGATTGTATCGTTTGCCATTTGTGGTGTAGCAGTTTTACC
TGTTCCGATTGCCCACACTGGTTCATAAGCCACAATTGTTTTTAATGCTTGTTCTTTAGA
AACACCTTCGAATGCTTTGTCTAATTGAACATCTAATACTTCTTTAGTCTTATTTGCTTC
TCTTGTATCTAATTGTTCACCGATACATAAAATTGGAGCTAAGTCATATTTAAATGCAGC
ATGCATTTTTAAATTTACGTCTTTGTCTGTTTCATTAAATAAACTACGACGTTCTGAGTG
TCCAATTAAGATATATTCAGCACCAGCAGTTTTTACTTGTGCAGGTGAGTTTTCTCCAGT
AAATGCGCCTTCATCAGCATAGTGCATATTTTGTGCACCAATACGGATATGTTCGCCTTC
ACGTTTTACTAATAAGTTTAATAAGATTGCTGGTGCACAAATAATAGACTCAACTTCGTC
TCTACTTGGTACTGCAGCATTTACAGCAAAAATGAATTCTAAAGCCTCATCTTTAGTTTT
AAACATTTTCCAGTTTGCTGCAATTACAGGTATTCTTTTTGCCAT


>protein sequence
MAKRIPVIAANWKMFKTKDEALEFIFAVNAAVPSRDEVESIICAPAILLNLLVKREGEHI
RIGAQNMHYADEGAFTGENSPAQVKTAGAEYILIGHSERRSLFNETDKDVNLKMHAAFKY
DLAPILCIGEQLDTREANKTKEVLDVQLDKAFEGVSKEQALKTIVAYEPVWAIGTGKTAT
PQMANDTIKDVRAKLSDLYGAEVAAQVRILYGGSVKPDNIESLLQESDIDGALIGGAALD
HKNFLTFTKAAKNK






















© Fisunov Lab of Proteomics, 2016.