ACL_RS06595

Uniprot: A9NHU6
Description: adenylosuccinate lyase
EC number: 4.3.2.2
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. {ECO:0000256|RuleBase:RU361172}.; CATALYTIC ACTIVITY: N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP. {ECO:0000256|RuleBase:RU361172}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2. {ECO:0000256|RuleBase:RU361172}.; PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2. {ECO:0000256|RuleBase:RU361172}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): purB
Gene names (synonym):
Mass: 48,788
Subunit structure [CC]:
Gene ontology (GO): (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate AMP-lyase (fumarate-forming) activity [GO:0070626]; N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity [GO:0004018]; 'de novo' AMP biosynthetic process [GO:0044208]; 'de novo' IMP biosynthetic process [GO:0006189]
Gene ontology IDs: GO:0004018; GO:0006189; GO:0044208; GO:0070626
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily. {ECO:0000256|RuleBase:RU361172}.
Protein families: Lyase 1 family, Adenylosuccinate lyase subfamily
Coiled coil:
Domain [FT]: DOMAIN 348 428 ADSL_C. {ECO:0000259|SMART:SM00998}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NHU6
MEROPS:
EnsemblBacteria KO: ABX81926
UniPathway: K01756
CDD:
Gene3D:
HAMAP: 1.10.275.10
InterPro:
PANTHER: IPR019468;IPR024083;IPR020557;IPR000362;IPR022761;IPR008948;IPR004769
PIRSF: PTHR11444
PRINTS:
PROSITE: PR00149
Pfam: PS00163
ProDom: PF10397;PF00206
SMART:
SUPFAM: SM00998
TIGRFAMs: SSF48557

1390333-1391626(+)

>nucleotide sequence
ATGATAAAAAGATATCGAACACCATTATTTGAAACTTTATGGAGTGATCAAAATCGCTAT
GACACTTTTTTGACAATTGAACTAGCTGTTGCCAAAGCGTGGTATTTATTAGGCAAATAT
GATGAAACAACTTACAATAAACTTACTAAAGCAACCTTTGATTTAGAATCAATCTATGCA
TTAGAAATAGAAACAAAACATGATATGATTGCATTTACAAGAACTGTCTCTAAAAGTTTG
GGTGCTGAGAAAATGTGGCTACACTATGGTTTAACATCTACAGATGTTGTCGACAGTGCA
CTAGCACTTATACTCAGGCAAGCCAATCAAGCAATTAGATCAAAAATTAATAAGTTATTG
TCAGTCTTAAAACAAAAAGCTTATACTTATAAAGACCAACCAACCATAGGTCGTACCCAT
GGCATCCACGCTGAAATTACATCTTTTGGATTAAAATTTGCACTATGGTACAGTGATCTA
AAACGGCTACTTTCTGTATTTGATGCTGCCTGTAAAAACATCGAAGTGATTAAAATATCA
GGCGCTGTTGGCAACTATGCGGCCAACACACCTCAACTAGAAAAAACAGTTTCCCGTCTA
TTAGATATAGCATCTGCAGACATTTCTACACAAGTACTTCAAAGAGACAGACATGCAAAC
TACATTTCAAGTATTGCACTATTGGGTAGTGAACTAGAAAAAATAGCCACTGAAATTAGA
CATTTATCACGTACTGAAGTATCTGAGGTAAATGAATACTTCAGTGTAGATCAAAAAGGT
TCTAGTGCAATGCCACATAAAAAAAATCCGGTATCTAGTGAAAATATATCTGGATTATCT
AGAGTCTTAAGAGGTTATGTTTTAACAGCTTTTGAAAATATTAATTTATGGCATGAAAGA
GATATTTCCCACTCCTCTGCTGAGCGTATCATATTAGCAGATGCAACAACATTAATTGAT
TATATGTTAGATCGTATAACAAATACGCTATCTAACTTAGAGGTCCACGCATTAAAACTA
CAAAACAATATAGATATAACTGGTGGTATCGTATTTAGTCAACATGTACTATCTTTATTG
ATTGATAAAGGACTTACAAGAGATCATGCGTATGATTTGGTTCAAAAGATTGCGACTGAT
GCCATGAGCAATCAAAAAGATTTTAAAACAGAACTTTTAAAAAGTTCAGATATCATGAAG
TTTATGACACCAGATGAACTGGATAAAGCGTTTGACTCAATCTATATATTAAGACATGTC
GATACCATATACTTAAAAGTCTTTAAAACATAA

>protein sequence
MIKRYRTPLFETLWSDQNRYDTFLTIELAVAKAWYLLGKYDETTYNKLTKATFDLESIYA
LEIETKHDMIAFTRTVSKSLGAEKMWLHYGLTSTDVVDSALALILRQANQAIRSKINKLL
SVLKQKAYTYKDQPTIGRTHGIHAEITSFGLKFALWYSDLKRLLSVFDAACKNIEVIKIS
GAVGNYAANTPQLEKTVSRLLDIASADISTQVLQRDRHANYISSIALLGSELEKIATEIR
HLSRTEVSEVNEYFSVDQKGSSAMPHKKNPVSSENISGLSRVLRGYVLTAFENINLWHER
DISHSSAERIILADATTLIDYMLDRITNTLSNLEVHALKLQNNIDITGGIVFSQHVLSLL
IDKGLTRDHAYDLVQKIATDAMSNQKDFKTELLKSSDIMKFMTPDELDKAFDSIYILRHV
DTIYLKVFKT