ACL_RS06700

Uniprot: A9NHW6
Description: tRNA N6-adenosine(37)-threonylcarbamoyltransferase complex transferase subunit TsaD
EC number: 2.3.1.234
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA. {ECO:0000255|HAMAP-Rule:MF_01445}.
Cofactor: COFACTOR: Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000255|HAMAP-Rule:MF_01445}; ; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01445}
Enzyme regulation:
Function [CC]: FUNCTION: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. {ECO:0000255|HAMAP-Rule:MF_01445}.
Pathway:
Active site:
Binding site: BINDING 166 166 Substrate. {ECO:0000255|HAMAP-Rule:MF_01445}.; BINDING 179 179 Substrate; via amide nitrogen. {ECO:0000255|HAMAP-Rule:MF_01445}.; BINDING 183 183 Substrate. {ECO:0000255|HAMAP-Rule:MF_01445}.; BINDING 272 272 Substrate. {ECO:0000255|HAMAP-Rule:MF_01445}.
Calcium binding:
DNA binding:
Metal binding: METAL 111 111 Iron. {ECO:0000255|HAMAP-Rule:MF_01445}.; METAL 115 115 Iron. {ECO:0000255|HAMAP-Rule:MF_01445}.; METAL 298 298 Iron. {ECO:0000255|HAMAP-Rule:MF_01445}.
Nucleotide binding:
Site:
Gene names (primary): tsaD
Gene names (synonym): gcp
Mass: 35,813
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; iron ion binding [GO:0005506]; metalloendopeptidase activity [GO:0004222]; N(6)-L-threonylcarbamoyladenine synthase [GO:0061711]; tRNA threonylcarbamoyladenosine modification [GO:0002949]
Gene ontology IDs: GO:0002949; GO:0004222; GO:0005506; GO:0005737; GO:0061711
Chain: CHAIN 1 331 tRNA N6-adenosine threonylcarbamoyltransferase. /FTId=PRO_1000145937.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the KAE1 / TsaD family. {ECO:0000255|HAMAP-Rule:MF_01445}.
Protein families: KAE1 / TsaD family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 133 137 Substrate binding. {ECO:0000255|HAMAP-Rule:MF_01445}.
EMBL: CP000896
ProteinModelPortal: A9NHW6
MEROPS:
EnsemblBacteria KO: ABX81946
UniPathway: K01409
CDD:
Gene3D:
HAMAP:
InterPro: MF_01445
PANTHER: IPR000905;IPR017861;IPR022450
PIRSF:
PRINTS:
PROSITE: PR00789
Pfam:
ProDom: PF00814
SMART:
SUPFAM:
TIGRFAMs:

1421037-1422033(-)

>nucleotide sequence
TTAGATAAAATTGATTGCAGAACTTCCCTTTAAATAATAATCTTTTAATGCACCCTGTTT
CTTAAATTGATAATAGGCTGCAATACCAATCATTGCAGCTTGATCTGTACAATATTTAAT
ACTTGGAGTAAGTATTTCTAAGTCTGTAATACGTTCTTTCATTTTAGTTCTTAAACCCTT
ATTTGCTGCAACACCGCCTGCAACAACTATTTGTTTAACACCTAAATTATTAGCTGCGTC
TTTTGTTTTTTGAACTAATACTTCAGTAACAGATTCTTGGAAAGATGCACAAACATCTTC
CACTTTAAAAGGCTCATTTCTTTGTTTCATATTATGTACTAAATTAAGTACAGAACTCTT
TAAACCTGAAAATGAGAAATTAAATGGTTCGTTTTTTAAGAAAGGTTTCGGTAAATTATA
GATTGCCTCACCTTGATGCGCTAATCTATCAACAACCGGTCCGCCTGGATAACCTAGACC
TAACATACGTGCTACCTTATCATAGGCCTCACCTACTGCATCATCTAAAGTTGTACCAAG
TAGTTCAATCTCTAAATGATCTTTAACTAATAAAAGTTCTGTATGCCCACCACTAACAAG
TAGTGTTAATAAAGGAAACTTCATTTCGCCTTCAATTTGTGCCGCATATATATGTCCTAA
TAAGTGGTTTACACCCATTAAAGGAATTTGATTAGCATAAGCAAAAGTGGTTGCTGCATT
TATTCCAACAAGTAGAGAACCAATTAATCCAGGGCCTTGTGTCACAGCAACTAAATCAAT
GTCTGATACTTTAATACCTGCATCTTTTATTGCTTTATCAAACATATATAACATAGACTC
AATATGCGCACGTGAAGCGATTTCAGGTACTACACCACCGTATGGTTGGTGTATTTTTAT
TTGTGATAAAACTGCATTAGATAAAACAGTCTTACCGTCTTTTGTAATTGCAACACTTGT
TTCATCACAAGATGTTTCAACAGATAAAATTATCAT

>protein sequence
MIILSVETSCDETSVAITKDGKTVLSNAVLSQIKIHQPYGGVVPEIASRAHIESMLYMFD
KAIKDAGIKVSDIDLVAVTQGPGLIGSLLVGINAATTFAYANQIPLMGVNHLLGHIYAAQ
IEGEMKFPLLTLLVSGGHTELLLVKDHLEIELLGTTLDDAVGEAYDKVARMLGLGYPGGP
VVDRLAHQGEAIYNLPKPFLKNEPFNFSFSGLKSSVLNLVHNMKQRNEPFKVEDVCASFQ
ESVTEVLVQKTKDAANNLGVKQIVVAGGVAANKGLRTKMKERITDLEILTPSIKYCTDQA
AMIGIAAYYQFKKQGALKDYYLKGSSAINFI