ACL_RS06775

Uniprot: A9NE02
Description: GTPase HflX
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00900};
Enzyme regulation:
Function [CC]: FUNCTION: GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis. {ECO:0000256|HAMAP-Rule:MF_00900}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 210 210 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00900}.; METAL 236 236 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00900}.
Nucleotide binding: NP_BIND 203 210 GTP. {ECO:0000256|HAMAP-Rule:MF_00900}.; NP_BIND 234 238 GTP. {ECO:0000256|HAMAP-Rule:MF_00900}.; NP_BIND 256 259 GTP. {ECO:0000256|HAMAP-Rule:MF_00900}.; NP_BIND 321 324 GTP. {ECO:0000256|HAMAP-Rule:MF_00900}.; NP_BIND 340 342 GTP. {ECO:0000256|HAMAP-Rule:MF_00900}.
Site:
Gene names (primary): hflX
Gene names (synonym):
Mass: 47,167
Subunit structure [CC]: SUBUNIT: Monomer. Associates with the 50S ribosomal subunit. {ECO:0000256|HAMAP-Rule:MF_00900}.
Gene ontology (GO): cytoplasm [GO:0005737]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; magnesium ion binding [GO:0000287]
Gene ontology IDs: GO:0000287; GO:0003924; GO:0005525; GO:0005737
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family. {ECO:0000256|HAMAP-Rule:MF_00900}.; SIMILARITY: Contains 1 Hflx-type G (guanine nucleotide-binding) domain. {ECO:0000256|HAMAP-Rule:MF_00900}.
Protein families: TRAFAC class OBG-HflX-like GTPase superfamily, HflX GTPase family
Coiled coil:
Domain [FT]: DOMAIN 197 362 Hflx-type G (guanine nucleotide-binding). {ECO:0000259|PROSITE:PS51705}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NE02
MEROPS:
EnsemblBacteria KO: ABX81962
UniPathway: K03665
CDD:
Gene3D:
HAMAP: 3.40.50.300
InterPro: MF_00900
PANTHER: IPR030394;IPR032305;IPR006073;IPR016496;IPR025121;IPR027417
PIRSF: PTHR10229
PRINTS: PIRSF006809
PROSITE: PR00326
Pfam: PS51705
ProDom: PF16360;PF13167;PF01926
SMART:
SUPFAM:
TIGRFAMs: SSF52540

1431224-1432475(+)

>nucleotide sequence
ATGCAAAAGCTTGATAGAGCTATTTTAGTTGCACTTAATTATCAAGGTAATTATGAATCA
TGTAAGCTGTCACTTGATGAATTAGAAGAGTTAGCAAAAGCTGTTCAAATTAAGACTGTA
GATAAAGTTATTCAATCCAATGATAAAATTGAACCTAAATTTTTTATTGGTAGTGGAAAA
GTTTTAGAAATCAAACAAATGATAGATATCTTAAATGTTGATATGGTTATTTTTGATGAT
ACCTTATCCCCTGCCCAAATCAGAAACTTAGAAGAGGCTTTAGATGTTCAAATATTTGAC
CGTGCCTTTTTAATTTTACAGATTTTTGCATTAAGAGCTCAAACCAAGCAGGCTATGCTT
GAAGTTGCACTTGCTCAAAAACTTTATATGCTACCACGTTTAGCTGGTATGGGTAAATCT
CTATCTAGACAAGGTGGTGGTTCATTTAACGCTAAAGGGCCTGGTGAAACCAAATTGGAA
ACAGATAGACGTCGATTAAATATGGAAATCACACGTTTAAGAGTATCGCTTGAAGGTATT
AAAAAAGAGCGTGGTGAATCTCGAAAACGTCGCTTTAAAAATAGTATTCCAGTTGTAGCA
CTTGTTGGTTATACCAATGCCGGTAAGTCTTCTTTAATGAATCATTTATCTAATCTATAT
GGTACAAATAAAACTGAAGTATTTGAAAAGGATATGTTATTTGCTACACTAGACACGAAA
TCTAAACGTATTCAAAAAGACAATCAACCACCTTTTATCTTAATTGATACCGTAGGTTTT
ATATCTAAACTACCACCAGAGTTAATTAAATCTTTTGAATCTACTCTAGCAGATATTGCT
TCAGCTGATCTTATCTTACATATTGTTGATGGTAAAAACTATAGTCCGCTACAAATAAAC
TTCACTAAACAAGTCATTAAAGATCTTGGTTTAGAAGAAACACCTAGATTACTTGTAGTG
ACTAAGAAAGATTTATTAGTACATCAACCAAATATATTAGAAGATTATTTATTAATCTCT
AATAAAACTGGTGAAGGCATGAATGACTTATATCAAGCAATTAGTGCACATATCTATAAA
GACTCAAGAATATTTAAACTACTACTTCCTTTTGATAAGGGTTCTATTTATGAACGTCTA
AAATCAGATACAACCATTATTGAAACAAACTACACTGCTGATGGTATGTTTGTTAAGGTT
GTACTCACACCTTATCTTTATAATTTATATAAACCTTATATCATGAATTAA

>protein sequence
MQKLDRAILVALNYQGNYESCKLSLDELEELAKAVQIKTVDKVIQSNDKIEPKFFIGSGK
VLEIKQMIDILNVDMVIFDDTLSPAQIRNLEEALDVQIFDRAFLILQIFALRAQTKQAML
EVALAQKLYMLPRLAGMGKSLSRQGGGSFNAKGPGETKLETDRRRLNMEITRLRVSLEGI
KKERGESRKRRFKNSIPVVALVGYTNAGKSSLMNHLSNLYGTNKTEVFEKDMLFATLDTK
SKRIQKDNQPPFILIDTVGFISKLPPELIKSFESTLADIASADLILHIVDGKNYSPLQIN
FTKQVIKDLGLEETPRLLVVTKKDLLVHQPNILEDYLLISNKTGEGMNDLYQAISAHIYK
DSRIFKLLLPFDKGSIYERLKSDTTIIETNYTADGMFVKVVLTPYLYNLYKPYIMN