ACL_RS06815

Uniprot: A9NE09
Description: tRNA-dihydrouridine synthase
EC number: 1.3.1.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000256|PIRNR:PIRNR006621};
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. {ECO:0000256|PIRNR:PIRNR006621}.
Pathway:
Active site: ACT_SITE 103 103 Proton donor. {ECO:0000256|PIRSR:PIRSR006621-1}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): duS
Gene names (synonym):
Mass: 37,128
Subunit structure [CC]:
Gene ontology (GO): flavin adenine dinucleotide binding [GO:0050660]; tRNA dihydrouridine synthase activity [GO:0017150]
Gene ontology IDs: GO:0017150; GO:0050660
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the dus family. {ECO:0000256|PIRNR:PIRNR006621}.
Protein families: Dus family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NE09
MEROPS:
EnsemblBacteria KO: ABX81969
UniPathway:
CDD:
Gene3D:
HAMAP: 1.10.1200.80;3.20.20.70
InterPro:
PANTHER: IPR013785;IPR024036;IPR004652;IPR001269;IPR018517
PIRSF: PTHR11082
PRINTS: PIRSF006621
PROSITE:
Pfam: PS01136
ProDom: PF01207
SMART:
SUPFAM:
TIGRFAMs:

1440898-1441909(-)

>nucleotide sequence
TTATGACTCAGTTATCTCAATAACGTTACCATGTGTGCTCATTACATCATCAAAATACAT
TTCTAAGTTTTCCATATACTCTGCTCTATTGGTTGTACGTGCTAATTTTGCACGTACTTG
ACTAGCCTTTGGTAAGCCTTTTAAATACCAAGGACCATGTGAACGCATTTCTAAACATGC
GGTATGTTCACCTTTGAGTTTTGCTAGATCATCAAAATGTCTAATCATTAAAGTTTTAAC
TTCTTCATATGTACGCACAGGTGTTTCTTCACCATTTAAATATGCATTAATTTCTCTAAA
TATCCATGGGTTACCAAGAGATGCTCTACCAATCATCACGGCGTCACAACCTGTATACTC
TAACATATGCTTAGCACTTTTACCATCCACAATATTCCCATTACCAATAACTGGAATAGA
TACTGCTTCTTTAACCGCTTTAATAACATCTAAATCAGGTTCACCTGAATAACCTTGTGC
ACGCGTACGTGCGTGTACAGTAATTAATGATGCGCCTGCTTTTTCAATCATTTTAGCAAC
CTCAACAGCATTAACAGATTCACTATCCCAACCACTTCTGATTTTTACAGTGACAGGTTT
AGATACTTTTTCTTTAATAGCTTTTACAATATCATATATTTTTTGAGGATCTTTCATTAA
AGATGCACCTGCTTGAGCACCAATTGCTACCTTTGGTACAGGGCATCCCATATTAATATC
AATAAAATCACAATTCGAATTTTTATCAATAAACATGGCTGCCTCAACCATTGTTTCTAA
ATCACTACCAAAGATTTGTTGAGCTGCTGGTTTTTCTTCAACCGTCATATAAAGCATTTG
AAGTGTTTTAGGATTTTGAAAAAAGATAGCCTTATCAGATACCATCTCTGCAAAAACAAC
CCCTGCACCTAATTGACGCGCCATTAATCTAAAAGGATGGTTAGATACTCCAGCCATAGG
CGCTAAGATTAATTTGTTTTCTACAACATAATTTCCCAATTTGAAACTCAT

>protein sequence
MSFKLGNYVVENKLILAPMAGVSNHPFRLMARQLGAGVVFAEMVSDKAIFFQNPKTLQML
YMTVEEKPAAQQIFGSDLETMVEAAMFIDKNSNCDFIDINMGCPVPKVAIGAQAGASLMK
DPQKIYDIVKAIKEKVSKPVTVKIRSGWDSESVNAVEVAKMIEKAGASLITVHARTRAQG
YSGEPDLDVIKAVKEAVSIPVIGNGNIVDGKSAKHMLEYTGCDAVMIGRASLGNPWIFRE
INAYLNGEETPVRTYEEVKTLMIRHFDDLAKLKGEHTACLEMRSHGPWYLKGLPKASQVR
AKLARTTNRAEYMENLEMYFDDVMSTHGNVIEITES