ACL_RS06845

Uniprot: A9NE15
Description: tRNA(Ile)-lysidine synthetase
EC number: 6.3.4.19
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H(2)O. {ECO:0000256|HAMAP-Rule:MF_01161, ECO:0000256|SAAS:SAAS00054827}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. {ECO:0000256|HAMAP-Rule:MF_01161, ECO:0000256|SAAS:SAAS00557872}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 18 23 ATP. {ECO:0000256|HAMAP-Rule:MF_01161}.
Site:
Gene names (primary): tilS
Gene names (synonym):
Mass: 47,601
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; ligase activity, forming carbon-nitrogen bonds [GO:0016879]; tRNA modification [GO:0006400]
Gene ontology IDs: GO:0005524; GO:0005737; GO:0006400; GO:0016879
Chain:
Signal peptide:
Domain [CC]: DOMAIN: The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. {ECO:0000256|HAMAP-Rule:MF_01161}.
Sequence similarities: SIMILARITY: Belongs to the tRNA(Ile)-lysidine synthase family. {ECO:0000256|HAMAP-Rule:MF_01161}.
Protein families: TRNA(Ile)-lysidine synthase family
Coiled coil:
Domain [FT]: DOMAIN 335 404 TilS_C. {ECO:0000259|SMART:SM00977}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NE15
MEROPS:
EnsemblBacteria KO: ABX81975
UniPathway: K04075
CDD:
Gene3D: cd01992
HAMAP: 3.40.50.620
InterPro: MF_01161
PANTHER: IPR012796;IPR020825;IPR014729;IPR011063;IPR012094;IPR012795
PIRSF: PTHR11807:SF2
PRINTS:
PROSITE:
Pfam:
ProDom: PF01171;PF11734
SMART:
SUPFAM: SM00977
TIGRFAMs: SSF56037

1445401-1446622(+)

>nucleotide sequence
ATGGCTTTAAAACTGAAACTTGATTCAACAAAATTATATGTTCTTGCAGTATCTGGAGGA
GTTGACTCCATGGTATTGTTTGATTTATTTGTTAAACATAATCTAAATTTTGTTGTTGTG
CATTTTAATCACCAAAAGCGCGCTGAATCTATTTTAGATCACGAACTGGTTGAATCCATG
TGTACACTTCATCATATTCCTTACCATTATATCAAATTAAAAATTAAAAATGGTAATTTT
CAGGAAGTGTCACGAAATGCTAGATATAAAAATCTAGAGTTTATTGCAGAACAATATCAT
ACACCATATATCGTTACAGCACATCATTTAGATGATTTAGCTGAAACTATTATGATGAAG
CTTATTAGAGGTTCTAATCTCTTAGGTTATGCTGCGATGCAAGAAGTAACCCATATCGAT
AAATTCATCTATTTAAAACCTTTATTGAACTATAGTAAAGAAGAAATTAGTCTTTATCAA
CAAACACATCAAATTAAATTCTATGAAGATCATACAAACTTAGAAGATACTTACTCAAGA
AATCGAGTAAGGCATCATCTCATCCCTTTATTAAAGGAAGAAAACGATGTTTTAACACAT
TTAAAAAACTTTTCTAAACAAAGTTATTTAGCAGCATCCTACATTAGGAAGGAGTCCAAT
AACTTCTTAAGTAAGGATTTATCTTTTCATTTATCGAGTTTTGATGCACTACATGATGCT
GTTAAAATGGACGTTATTTCCTTAATATTTGAGTCTTTAAAAATCGATAAAACATTTAAG
AAAATTGAAACTATTTTAGCACAATTAAAGTCAATAAAGCCTAATATTAATATCACGCTT
AATAAGCACTATCATATGATAAAAGCCTATGATTTTGTCTACATAGAGCATATTGATAAA
AATAAGACTCGAAATGATGAGAATTGTCTCATGATTTCCCACAATAAAGTAGACCTCCCG
AAGATTTGGGTAGAATTATGTTATAATAAACTGGATTTTCCTATTCAATTAAGGAAGCGT
AAACCGGGGGATCGTTTATCCTTTACCTACGGTAGTAAGAAATTAAAGGACTATTTAATT
GATAAGAAGATTGCAATTAGTGATAGAAATAATCTAAATGTCGTTGTGGATAGTTCCGGT
ACCATTTTATGGATACCTGGACTTTATTTAAATGAGACCCTAGGGTCAGAACATAAAATT
TACTTATCTATAAAGGAGTAA

>protein sequence
MALKLKLDSTKLYVLAVSGGVDSMVLFDLFVKHNLNFVVVHFNHQKRAESILDHELVESM
CTLHHIPYHYIKLKIKNGNFQEVSRNARYKNLEFIAEQYHTPYIVTAHHLDDLAETIMMK
LIRGSNLLGYAAMQEVTHIDKFIYLKPLLNYSKEEISLYQQTHQIKFYEDHTNLEDTYSR
NRVRHHLIPLLKEENDVLTHLKNFSKQSYLAASYIRKESNNFLSKDLSFHLSSFDALHDA
VKMDVISLIFESLKIDKTFKKIETILAQLKSIKPNINITLNKHYHMIKAYDFVYIEHIDK
NKTRNDENCLMISHNKVDLPKIWVELCYNKLDFPIQLRKRKPGDRLSFTYGSKKLKDYLI
DKKIAISDRNNLNVVVDSSGTILWIPGLYLNETLGSEHKIYLSIKE