ACL_RS06940

Uniprot: A9NE34
Description: tRNA uridine(34) 5-carboxymethylaminomethyl synthesis GTPase MnmE
EC number: 3.6.-.-
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000255|HAMAP-Rule:MF_00379}; ; Note=Binds 1 potassium ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00379}
Enzyme regulation:
Function [CC]: FUNCTION: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-Rule:MF_00379}.
Pathway:
Active site:
Binding site: BINDING 22 22 Formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_00379}.; BINDING 83 83 Formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_00379}.; BINDING 122 122 Formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_00379}.; BINDING 448 448 Formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_00379}.
Calcium binding:
DNA binding:
Metal binding: METAL 229 229 Potassium. {ECO:0000255|HAMAP-Rule:MF_00379}.; METAL 233 233 Magnesium. {ECO:0000255|HAMAP-Rule:MF_00379}.; METAL 248 248 Potassium; via carbonyl oxygen. {ECO:0000255|HAMAP-Rule:MF_00379}.; METAL 250 250 Potassium; via carbonyl oxygen. {ECO:0000255|HAMAP-Rule:MF_00379}.; METAL 253 253 Potassium. {ECO:0000255|HAMAP-Rule:MF_00379}.; METAL 254 254 Magnesium. {ECO:0000255|HAMAP-Rule:MF_00379}.
Nucleotide binding: NP_BIND 229 234 GTP. {ECO:0000255|HAMAP-Rule:MF_00379}.; NP_BIND 248 254 GTP. {ECO:0000255|HAMAP-Rule:MF_00379}.; NP_BIND 273 276 GTP. {ECO:0000255|HAMAP-Rule:MF_00379}.
Site:
Gene names (primary): mnmE
Gene names (synonym): trmE
Mass: 49,782
Subunit structure [CC]: SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits. {ECO:0000255|HAMAP-Rule:MF_00379}.
Gene ontology (GO): cytoplasm [GO:0005737]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; metal ion binding [GO:0046872]; tRNA modification [GO:0006400]
Gene ontology IDs: GO:0003924; GO:0005525; GO:0005737; GO:0006400; GO:0046872
Chain: CHAIN 1 448 tRNA modification GTPase MnmE. /FTId=PRO_1000079999.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. {ECO:0000255|HAMAP-Rule:MF_00379}.; SIMILARITY: Contains 1 TrmE-type G (guanine nucleotide-binding) domain. {ECO:0000305}.
Protein families: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, TrmE GTPase family
Coiled coil:
Domain [FT]: DOMAIN 219 369 TrmE-type G.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NE34
MEROPS:
EnsemblBacteria KO: ABX81994
UniPathway: K03650
CDD:
Gene3D:
HAMAP: 1.20.120.430;3.30.1360.120;3.40.50.300
InterPro: MF_00379
PANTHER: IPR031168;IPR018948;IPR006073;IPR004520;IPR027368;IPR025867;IPR027417;IPR005225;IPR027266
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51709
ProDom: PF01926;PF12631;PF10396
SMART:
SUPFAM:
TIGRFAMs: SSF52540

1463026-1464373(-)

>nucleotide sequence
CTATTTTCCAAGACAGAATTTAGAAAATAATTCCGTCAATAAATCACCATATTGATGATT
CCCTAGTATTTCACCTAAGCGGTTCCAAGCAGTTGTAAGATCCACTGCATAGATATCCAC
AGGCATATCATGGTGGATAGCGTTTATAACGCTTTCTAGTGATGTTTTAGCCTCTTTTAG
CTTTTGTATATGGCGGACATTAGATAAGTAATTAAAGTCCTTATCTACAAGGTTATCAAG
TTTTAAAGTTTTAAGAATTTCTGACTCTAAAACACTTAATCCTTCTTTTTCTAATGTAGA
TATTTGAACATTTGTATCTATTTCTAAATATGACGGTAAATCTTTTTTATTTCCAATAAT
AATTCTATTTTTATGTTCAGTTAATGTTAGAAGCATCTCATCTTCTTTAGTTAGTTTTTG
ACTTAAGTCTAAAACCAATAAAACAAGTTCTGCTTCATGAATTGCTTTTCTACTTCGATC
TACACCAATCTTTTCGATGGTATCTAGTGCATCTCTAATACCTGCAGTATCAATTAACTG
TAAGGTGACACCATCAAGGTTTACAAATGCATCAATCGTATCTCTTGTAGTACCAGCAAT
ATCTGAGACAATGGCGCGCTCTTCATTAAGAAGTGCGTTTAATAAACTTGATTTACCTAC
ATTAGGGCGTCCAACGATAACCGTTTTAACACCTTCTCTAATGTACTGAGTACGTTTAGA
TTTGGTTAGAATATCATTAATCTCATCTAACAAATCGTTGACTCTTGGATAAATAAGTTC
TTTACTCATGATGATAGCATCATCATATTCTGGATAATCAATGTTCACTTCAATTTGTGC
AATTAAAGTTAATACTTTGGATTTTAAATTATCAATCATGGATGAAGTTGCTTTATTTAA
AGCTTTATTGGCAACCTTAATCGCATTTTCGTTTGTAGCATGAATAATGTCCATAATCGC
TTCTGCTTGAACAAGGTCAATTCGACCATTTAAAAATGCTCTTTTTGAAAACTCACCCGG
TTGTGCAAGTTCAATACCTGTTTCTAAAATACGCTCCAATACTTTTTGAGTGATTAATAT
ACCACCGTGTGTAGATATTTCTACAACATCTTCTTTTGTAAAAGACTTAGGTGCGATAAA
AACAGAGATCATAACTTCATCTAATATAGACCCATCTTCATTTAAGATGTGTCCATAATG
AATGGTATGACTCTTAACCTTGTTTAAGTTCTTACCCTTAAATATTTGATTTACTTTTGA
AATTGCTTCATCACCAGATACCCTAATTACAGAAATACCTGCAGTCCCTAATGGGGTTGC
GATTGCAGCAATGGTTTGAAATTGCAT

>protein sequence
MQFQTIAAIATPLGTAGISVIRVSGDEAISKVNQIFKGKNLNKVKSHTIHYGHILNEDGS
ILDEVMISVFIAPKSFTKEDVVEISTHGGILITQKVLERILETGIELAQPGEFSKRAFLN
GRIDLVQAEAIMDIIHATNENAIKVANKALNKATSSMIDNLKSKVLTLIAQIEVNIDYPE
YDDAIIMSKELIYPRVNDLLDEINDILTKSKRTQYIREGVKTVIVGRPNVGKSSLLNALL
NEERAIVSDIAGTTRDTIDAFVNLDGVTLQLIDTAGIRDALDTIEKIGVDRSRKAIHEAE
LVLLVLDLSQKLTKEDEMLLTLTEHKNRIIIGNKKDLPSYLEIDTNVQISTLEKEGLSVL
ESEILKTLKLDNLVDKDFNYLSNVRHIQKLKEAKTSLESVINAIHHDMPVDIYAVDLTTA
WNRLGEILGNHQYGDLLTELFSKFCLGK