ACL_RS07295

Uniprot: A9NGS5
Description: DNA primase
EC number: 2.7.7.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|PIRSR:PIRSR002811-1}; ; Note=Binds 1 zinc ion per monomer. {ECO:0000256|PIRSR:PIRSR002811-1}
Enzyme regulation:
Function [CC]: FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00079980}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 257 257 Magnesium 1; catalytic. {ECO:0000256|HAMAP-Rule:MF_00974}.; METAL 301 301 Magnesium 1; catalytic. {ECO:0000256|HAMAP-Rule:MF_00974}.; METAL 301 301 Magnesium 2. {ECO:0000256|HAMAP-Rule:MF_00974}.; METAL 303 303 Magnesium 2. {ECO:0000256|HAMAP-Rule:MF_00974}.
Nucleotide binding:
Site:
Gene names (primary): dnaG
Gene names (synonym):
Mass: 66,355
Subunit structure [CC]:
Gene ontology (GO): primosome complex [GO:1990077]; DNA binding [GO:0003677]; DNA primase activity [GO:0003896]; magnesium ion binding [GO:0000287]; zinc ion binding [GO:0008270]
Gene ontology IDs: GO:0000287; GO:0003677; GO:0003896; GO:0008270; GO:1990077
Chain:
Signal peptide:
Domain [CC]: DOMAIN: Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
Sequence similarities: SIMILARITY: Belongs to the DnaG primase family. {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00534519}.; SIMILARITY: Contains 1 Toprim domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
Protein families: DnaG primase family
Coiled coil:
Domain [FT]: DOMAIN 251 332 Toprim. {ECO:0000259|PROSITE:PS50880}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NGS5
MEROPS:
EnsemblBacteria KO: ABX81555
UniPathway: K02316
CDD:
Gene3D:
HAMAP: 3.90.580.10;3.90.980.10
InterPro: MF_00974
PANTHER: IPR013264;IPR006295;IPR030846;IPR006171;IPR002694
PIRSF:
PRINTS: PIRSF002811
PROSITE:
Pfam: PS50880
ProDom: PF08275;PF01807
SMART:
SUPFAM: SM00493;SM00400
TIGRFAMs:

970653-972381(-)

>nucleotide sequence
TCATTTTATTTTTACCTGCTTTCTGAGTTCATCAATCGCCTTTAAGGTGCGATTCATTTC
AAGATGGTCCGTTATGGACTCTGTTTTCTTTATTAAGGATTGTATTTCTAATTGTTTCTT
ATAATTTTGAAAATGTACTAATATATCATTGAGTGTTTTATCATTTTGAATTAATAAGTT
TTTACAATAGTCTGTTGATTTACAATATGTTTCAACTAATTCTATTTCTTTAGTATATCC
CATTTTAAATGCTTCAAGGTTTAATGCATCATCCGAATTAATCTCATAATAATTCATTAA
ATCTTTATAAAGTTCACGTTTTTTAGGGTCTGTAATATCTGTTAATAAAATACTATGTCT
AATCTTATCTAGATATTTACGCTGTTTAAGTAAATCCATAATGATGATATCAATAGAACG
TTCAGCACGACTTATGACTTTCCTATCAATAGGTTTTTGTTTGACTGGAAGTGATGAACT
ATGTTCATTAATAAGTAGTTGTATGCCTAACTCATCAAATGATCTTCTCTCGTATAAATC
AATGATGGTCGGATCACTTCCAACTAATAACTGCTTCATTTCTTGCTTAAACTTTGTAAT
ATCATCTGACTTTTTAAAGTCTTTATCTTTTTGATAGTATATATATCCAAATTGGACTGG
GTCAAGAAGTTTGTTAAAGAGTGCTTGATATTTATCTACACCATGTTTTAAAACAAAATC
ATCCGGATCCATTTTATTTGGTAAATTTAATATGGAAATACTTAGTCCCTGACGTTTTAA
AATAGGTATTGCATGAAGGGTAGCTTGTATACCAGGGTTATCTCCGTCATATGCAATCAC
TACATGATCTGTAATACGTCTAATCAATTTTGCTTGATCACTTGTAATTGCAGTTCCCAT
AGTTGCAACACTTGCTTCAAAACCTGCCTTATAGGATGCAAAAACGTCGAAGAAACCTTC
ATGTAGAATCACATGTTTTTGCTTAACTACTGGTCTAATACTTTCAAAATAGTGATAGAG
TGTTAACCCTTTTTTAAAGATTGGTGTTTCAGTTGAGTTAATGTATTTAGCTTTCTCATT
TTTATTTAAAGTTCTTCCTGAAAAGGCAATAATCTGACCATGTTCATCTTTAATCGGAAA
AGTGATACGATTTCTAAATACATCGTAATAGTGACCTTCTTCATTTTGTTTAACTAAACC
TAAACTCATCATGTCACTAACACTATGATTTTTAGATCTTAGCATTTTATATAAACTATC
TATTTGATCTGGTGCATAACCCACTTCAAAGTGATGAATTAACTCATCACTTAACTGTCT
ATTTGTTAAATAGTCATAGGCTAATTTACCGGATTCAGAGTTCTTAAGCGCAAACTGATA
AAAACTAGCTGCATCATTCATGACATCATGTAGATGTTTAAAAGGATTATTTTGAACTTT
AGTTTGACCAAATTCAATACCCAGTTCTTGGGCTAATTCCTTTGCTGCATCTGCATTACT
AATGTTTTTAATTTGAGCTAAGAAAGTTATCGGTGCACCACCTTTTTTACAGCCAAAACA
AAATGCTATATTCTTTTCTGGTGTTACAGAAAATGATGGTGTTTTTTCATCATGAAAAGG
ACATAGTCCCATGTAGTTTTTACCACGTTTGGTTAAGTCTACATATTTGGAGACTAATGC
AACGATATCTGTTTGTTCATTAATTTTTTGATAGATATTATCTTGCAT

>protein sequence
MQDNIYQKINEQTDIVALVSKYVDLTKRGKNYMGLCPFHDEKTPSFSVTPEKNIAFCFGC
KKGGAPITFLAQIKNISNADAAKELAQELGIEFGQTKVQNNPFKHLHDVMNDAASFYQFA
LKNSESGKLAYDYLTNRQLSDELIHHFEVGYAPDQIDSLYKMLRSKNHSVSDMMSLGLVK
QNEEGHYYDVFRNRITFPIKDEHGQIIAFSGRTLNKNEKAKYINSTETPIFKKGLTLYHY
FESIRPVVKQKHVILHEGFFDVFASYKAGFEASVATMGTAITSDQAKLIRRITDHVVIAY
DGDNPGIQATLHAIPILKRQGLSISILNLPNKMDPDDFVLKHGVDKYQALFNKLLDPVQF
GYIYYQKDKDFKKSDDITKFKQEMKQLLVGSDPTIIDLYERRSFDELGIQLLINEHSSSL
PVKQKPIDRKVISRAERSIDIIIMDLLKQRKYLDKIRHSILLTDITDPKKRELYKDLMNY
YEINSDDALNLEAFKMGYTKEIELVETYCKSTDYCKNLLIQNDKTLNDILVHFQNYKKQL
EIQSLIKKTESITDHLEMNRTLKAIDELRKQVKIKW