GCW_00045

Uniprot: A0A0F6CJR9
Description: 16S rRNA methyltransferase
EC number: 2.1.1.182
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. {ECO:0000256|HAMAP-Rule:MF_00607}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. {ECO:0000256|HAMAP-Rule:MF_00607}.
Pathway:
Active site:
Binding site: BINDING 23 23 S-adenosyl-L-methionine; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00607}.; BINDING 25 25 S-adenosyl-L-methionine; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00607}.; BINDING 50 50 S-adenosyl-L-methionine; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00607}.; BINDING 72 72 S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_00607}.; BINDING 94 94 S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_00607}.; BINDING 116 116 S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_00607}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): ksgA
Gene names (synonym): rsmA
Mass: 30,783
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity [GO:0052908]; RNA binding [GO:0003723]; rRNA (adenine-N6,N6-)-dimethyltransferase activity [GO:0000179]
Gene ontology IDs: GO:0000179; GO:0003723; GO:0005737; GO:0052908
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily. {ECO:0000256|HAMAP-Rule:MF_00607, ECO:0000256|SAAS:SAAS00540765}.
Protein families: Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family, RsmA subfamily
Coiled coil:
Domain [FT]: DOMAIN 30 199 rADc. {ECO:0000259|SMART:SM00650}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99341
UniPathway: K02528
CDD:
Gene3D:
HAMAP: 1.10.8.100;3.40.50.150
InterPro: MF_00607
PANTHER: IPR001737;IPR023165;IPR020596;IPR020598;IPR011530;IPR029063
PIRSF: PTHR11727
PRINTS:
PROSITE:
Pfam: PS01131;PS51689
ProDom: PF00398
SMART:
SUPFAM: SM00650
TIGRFAMs: SSF53335
10048-10855(+)

>nucleotide sequence
ATGATCAACAAGATTAATAAGTTTTTTAAAAACAACGAATTTTCGCCTTCTAAACAAAGA
GGGCAAAATTTTTTAATTGACCAAAATATTATTAATAAGGTTGTTGAAGCAGTAAACAAG
ATTAACCCTTCCAAAGTCTTAGAGATTGGCCCTGGGCTAGGTGCTATTAGCGAACAATTA
ATTAAAAGGTTTGCTGATAATTATTACGCCATTGAGTTAGATAAGAAGTTATTTCATCAC
CTAAATGAAGGGTTGTTAAAAGATCATATTTTACATGCTGATGCTTTAGAGATTGATTGA
AAATCAATCTTTGATAATTTAGGTGATAATCCCACAATGGTAGGAAATTTACCTTATAAT
ATCTCATCTAAGTTGATTAAGAAGTTTATCTTATCAACTTATCGTTGTGCAATTATTATG
GTGCAAAAAGAGATGGGATTAAGGTTATTAGCTAAGATTAATTCCAAAGATTATTCAGCA
TTTAGTGCTTTGTGTCAATACTCTTTAAATGTTAGCAAGATTATTGAGATCAATGAAACT
GCTTTCATTCCCGAACCAAAAGTAAGATCTACTTTGTTGTTTTTAGAAAAAAAAGACATA
GCTTTTAATGAAGGATATGAGAAGTTTTTAAAATTGATCTTTTTATCTAGAAGAAAAACA
ATCTTAAATAATCTTAAAAATAATTATGATCCCAAGCTAATAATACAAAGTTTAGTCAAC
CTTGGTTTTAAAGAAACAACCAGAGCTCAAGAGTTATCACCTACCCAACTGTTTAGCTTA
TATGAATCATTATCGAAGTTATGCTAA

>protein sequence
MINKINKFFKNNEFSPSKQRGQNFLIDQNIINKVVEAVNKINPSKVLEIGPGLGAISEQL
IKRFADNYYAIELDKKLFHHLNEGLLKDHILHADALEIDWKSIFDNLGDNPTMVGNLPYN
ISSKLIKKFILSTYRCAIIMVQKEMGLRLLAKINSKDYSAFSALCQYSLNVSKIIEINET
AFIPEPKVRSTLLFLEKKDIAFNEGYEKFLKLIFLSRRKTILNNLKNNYDPKLIIQSLVN
LGFKETTRAQELSPTQLFSLYESLSKLC