GCW_00050

Uniprot: A0A0F6CLK0
Description: diphosphocytidyl-2C-methyl-D-erythritol2- phosphate synthase
EC number: 2.7.1.148
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. {ECO:0000256|SAAS:SAAS00090328}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. {ECO:0000256|SAAS:SAAS00090332}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): ispE
Gene names (synonym):
Mass: 27,980
Subunit structure [CC]:
Gene ontology (GO): 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity [GO:0050515]; ATP binding [GO:0005524]; isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway [GO:0019288]; terpenoid biosynthetic process [GO:0016114]
Gene ontology IDs: GO:0005524; GO:0016114; GO:0019288; GO:0050515
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the GHMP kinase family. IspE subfamily. {ECO:0000256|SAAS:SAAS00571601}.
Protein families: GHMP kinase family, IspE subfamily
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99972
UniPathway: K00919
CDD:
Gene3D:
HAMAP: 3.30.230.10;3.30.70.890
InterPro:
PANTHER: IPR013750;IPR004424;IPR020568;IPR014721
PIRSF: PTHR20861:SF2
PRINTS:
PROSITE:
Pfam:
ProDom:
SMART:
SUPFAM:
TIGRFAMs: SSF54211;SSF55060
10829-11555(+)

>nucleotide sequence
ATGAATCATTATCGAAGTTATGCTAAAGTTAATTTTTATCTAAAAATTAAGGAATACTGT
CCAAAAGTTAAAAAGCATAAACTTGAGTCCAAGTTAGTTCTTGTTAAAGATCTTTATGAT
GATCTTTATTTAGAAAAATCGAACCAAACTAAGATTAAGTATTTTGATCAAAATAACAAC
TCTTTAACTTTTAGTGATGATATTTTAGTTAGGACTAAAAAATTTTTAGAAGATAAGTTA
AATCAAACTTTTGAATTTAAAGCGAAGGTGATTAAAAGAATTCCGACCCTTTCTGGACTT
GGCTCAGCTCCAAGTAATGCTGCTGTTTTGATTAATTGAATTTACCAAGAATTTGGAATC
CTTAACCCGGTTAGTTATTATGAAATAGCTACTGAACTAGGTAGTGATATTCCCTTTTTC
TTATCCTTACACGATTGTGCAATTATTAAGAATTTTGGGGATCAAGTAGAGCAATCTATT
TTGGATGGGTATGAAACAGAAAAGATCATTTTTAACAAACAAAAACCTAGCACAAAAAAG
ATCTTTGAACTCTTAGATTATTCTCAAATTAAAAAAGAAAATCTTAATGATTTAGAACAA
CCGTTTTTTAGTTTATTTCCCGAATTATTACCTTCTTTTAACCAATTAAAAAATGACAGC
AATCATGTTGTGTTAGCAGGAGCTGGAAGCTGCTTTGTCGTATTTAGAAAGCTAAATGAA
ATATAA

>protein sequence
MNHYRSYAKVNFYLKIKEYCPKVKKHKLESKLVLVKDLYDDLYLEKSNQTKIKYFDQNNN
SLTFSDDILVRTKKFLEDKLNQTFEFKAKVIKRIPTLSGLGSAPSNAAVLINWIYQEFGI
LNPVSYYEIATELGSDIPFFLSLHDCAIIKNFGDQVEQSILDGYETEKIIFNKQKPSTKK
IFELLDYSQIKKENLNDLEQPFFSLFPELLPSFNQLKNDSNHVVLAGAGSCFVVFRKLNE
I