GCW_00205

Uniprot: A0A0F6CJU3
Description: excinuclease ABC subunit B
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage. {ECO:0000256|HAMAP-Rule:MF_00204, ECO:0000256|SAAS:SAAS00341756}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 40 47 ATP. {ECO:0000256|HAMAP-Rule:MF_00204}.
Site:
Gene names (primary): uvrB
Gene names (synonym):
Mass: 76,897
Subunit structure [CC]: SUBUNIT: Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex. {ECO:0000256|HAMAP-Rule:MF_00204, ECO:0000256|RuleBase:RU003587, ECO:0000256|SAAS:SAAS00009162}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; excinuclease ABC activity [GO:0009381]; helicase activity [GO:0004386]; nucleotide-excision repair [GO:0006289]; SOS response [GO:0009432]
Gene ontology IDs: GO:0003677; GO:0004386; GO:0005524; GO:0005737; GO:0006289; GO:0009381; GO:0009432
Chain:
Signal peptide:
Domain [CC]: DOMAIN: The beta-hairpin motif is involved in DNA binding. {ECO:0000256|HAMAP-Rule:MF_00204}.
Sequence similarities: SIMILARITY: Belongs to the UvrB family. {ECO:0000256|HAMAP-Rule:MF_00204, ECO:0000256|RuleBase:RU003587, ECO:0000256|SAAS:SAAS00538712}.; SIMILARITY: Contains 1 UVR domain. {ECO:0000256|HAMAP-Rule:MF_00204, ECO:0000256|RuleBase:RU003587}.; SIMILARITY: Contains 1 helicase ATP-binding domain. {ECO:0000256|HAMAP-Rule:MF_00204}.; SIMILARITY: Contains 1 helicase C-terminal domain. {ECO:0000256|HAMAP-Rule:MF_00204}.
Protein families: UvrB family
Coiled coil:
Domain [FT]: DOMAIN 27 162 Helicase ATP-binding. {ECO:0000259|PROSITE:PS51192}.; DOMAIN 432 594 Helicase C-terminal. {ECO:0000259|PROSITE:PS51194}.; DOMAIN 622 657 UVR. {ECO:0000259|PROSITE:PS50151}.
Motif: MOTIF 93 116 Beta-hairpin. {ECO:0000256|HAMAP-Rule:MF_00204}.
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99365
UniPathway: K03702
CDD:
Gene3D:
HAMAP: 3.40.50.300;4.10.860.10
InterPro: MF_00204
PANTHER: IPR006935;IPR014001;IPR001650;IPR027417;IPR001943;IPR004807;IPR024759
PIRSF: PTHR24029
PRINTS:
PROSITE:
Pfam: PS51192;PS51194;PS50151
ProDom: PF00271;PF04851;PF02151;PF12344
SMART:
SUPFAM: SM00487;SM00490
TIGRFAMs: SSF46600;SSF52540

In one protein complex with GCW_02010 GCW_00470 51950-53945(+)

>nucleotide sequence
ATGGCAGATAAAAAATTTAAACTAGTTTCTAAAAATAAACCAGCTGGACACCAACCAGAA
GCTATTAAAAAACTTGTTGATGGGATTAATAAAAATAAAAAGTATCAAACCCTATTAGGT
GCTACTGGTACAGGAAAAACTTTTACAATTGCCAATGTAATTGAAAAAACTCAAAAAAAG
ACGCTAATTTTAGCTCATAACAAGACACTAGCAGCACAGCTATATCTAGAATTTAAGGAA
TTATTTCCTAACAATGCTGTTGAATATTTTGTTTCTTATTTCGATTTTTATCAACCTGAA
GCATATATTCCAAGAACAGACATGTACATCGAAAAAAGTTCAGTTACTAATGACGAAATT
GAGATGTTAAGATTAGCATCATTAAACTCTTTATCAACTAGAAACGATGTAATTGTTGTT
GCTTCTGTTGCTTGCATTTATCCTGCTGCTAATCCCGAAGACTTCGATATTTACCGCATA
ATTTTAAAAGTTGGTAACACTCTTAAGCTTTCAGATTTAAAAGAAAATTTAATTAGATTA
AATTACGCTAGAAGTCCTGAATGTAACGAACCAGGAACATTTAGAATCAAAGGAGATGTT
GTTGATATCTTTCCTGGTTATGTAAATGACCATATTATTAGGTTAAGTTTTTTTGGTGAT
GAATTAGAAGAAATCAGAAAAATTCATCCAACTGATAGTAGTGTTATTGAAAAATATACT
TCTTATGTATTAGGTCCAGCTAATGAATATATCTTAAATTTTGAACGTAAAGACACCGCG
ATAAAAAGAATTCAAGAAGAATTGATGTTTCGAGTTCAAGAATTTAAAAATCAACAAAAA
CTTGTAGAAGCGCAAAGACTTCAACAAAGAACAGAATACGATATTGATGCGATTAAAGAA
TTCGGGTTCTGTAACGGGATTGAAAACTACGCTTTTCACTTAGAATTGCGTGAAAAAGGT
TCTACTCCTTGAACGCTATTTGATTTCTTTGGTGATGATTGATTAATGGTAATCGACGAA
TCGCATATTTCTGTTCCCCAAGTTAAGGGTATGTTTAACACCGATAAATCAAGAAAAACT
ACTTTAGTAGAGTATGGTTTTAGATTACCTTCAGCCCTAGAAAACCGTCCTTTAAATTAC
GACGAATTTAGCAATAAATCTGATCAGGTAATATTTGTTTCAGCCACCCCTAATGATGAA
GAGATCAAACTATCTAATAACGAAATAATTGAACAAATAGTTCGACCTACAGGTTTGTTA
GATCCAACTGTTGAAATCAGACCAAGATTAGATCAAATTAATGATCTAATGAATGAATTG
AAGAAACAAAAAGATAAAAACGAACGGACTTTTATTACAGTTACAACGATTAAAATGGCA
GAAGATCTAACTGAATATTTAAAAGAACGCAACTTCAAATGTGCTTATATTCACAACGAA
TTAAAAACTCTAGAACGATCATTAATTCTTAATGATCTAAGAAGAGGTAAGTATGATTGT
GTTGTCGGAATTAACCTCTTACGTGAAGGATTAGATATTCCAGAAGTTTCATTAGTATGT
ATTTTTGACGCTGACAAACCTGGTTATTTCAGAAGTGATAAAGCTTTAATTCAAACTATA
GGTAGAGCAGCACGTAACCAAAATGGGCGCGTGATTATGTATGCTGATGAGATGACTAAA
GCGATGAAAATCGCTGTTGATGAAACTAATCGTAGACGTAAGATCCAAGAGAAATTTAAC
AAAGATCATAAGATCACACCTAAAACTATTATCAAACCAATCTATGATGATTTAAAAAAC
AAAGCATCACACAAACAGATTGAAGAAGTGATGCGCAAAACTAAAGCTAAAGGTGATAAG
TTTATTAAGATGATTGAAGATCTAAGAAACGAAATGCTTGAAGCTGCTAAGAACCAAAAT
TATGAGCACGCAGCTAGTTTAAGAGATTTAATCATCGAATTAGAAACCCAACAACTAAGT
AAGACTAATAAATAG

>protein sequence
MADKKFKLVSKNKPAGHQPEAIKKLVDGINKNKKYQTLLGATGTGKTFTIANVIEKTQKK
TLILAHNKTLAAQLYLEFKELFPNNAVEYFVSYFDFYQPEAYIPRTDMYIEKSSVTNDEI
EMLRLASLNSLSTRNDVIVVASVACIYPAANPEDFDIYRIILKVGNTLKLSDLKENLIRL
NYARSPECNEPGTFRIKGDVVDIFPGYVNDHIIRLSFFGDELEEIRKIHPTDSSVIEKYT
SYVLGPANEYILNFERKDTAIKRIQEELMFRVQEFKNQQKLVEAQRLQQRTEYDIDAIKE
FGFCNGIENYAFHLELREKGSTPWTLFDFFGDDWLMVIDESHISVPQVKGMFNTDKSRKT
TLVEYGFRLPSALENRPLNYDEFSNKSDQVIFVSATPNDEEIKLSNNEIIEQIVRPTGLL
DPTVEIRPRLDQINDLMNELKKQKDKNERTFITVTTIKMAEDLTEYLKERNFKCAYIHNE
LKTLERSLILNDLRRGKYDCVVGINLLREGLDIPEVSLVCIFDADKPGYFRSDKALIQTI
GRAARNQNGRVIMYADEMTKAMKIAVDETNRRRKIQEKFNKDHKITPKTIIKPIYDDLKN
KASHKQIEEVMRKTKAKGDKFIKMIEDLRNEMLEAAKNQNYEHAASLRDLIIELETQQLS
KTNK