GCW_00245

Uniprot: A0A0F6CJV1
Description: thymidylate synthase
EC number: 2.1.1.45
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. {ECO:0000256|HAMAP-Rule:MF_00008, ECO:0000256|SAAS:SAAS00539860}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Provides the sole de novo source of dTMP for DNA biosynthesis. {ECO:0000256|HAMAP-Rule:MF_00008}.
Pathway: PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000256|HAMAP-Rule:MF_00008, ECO:0000256|SAAS:SAAS00217161}.
Active site: ACT_SITE 170 170 {ECO:0000256|HAMAP-Rule:MF_00008}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): thyA
Gene names (synonym):
Mass: 33,846
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00008, ECO:0000256|SAAS:SAAS00539856}.
Gene ontology (GO): cytoplasm [GO:0005737]; thymidylate synthase activity [GO:0004799]; dTMP biosynthetic process [GO:0006231]; dTTP biosynthetic process [GO:0006235]
Gene ontology IDs: GO:0004799; GO:0005737; GO:0006231; GO:0006235
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. {ECO:0000256|HAMAP-Rule:MF_00008}.
Protein families: Thymidylate synthase family, Bacterial-type ThyA subfamily
Coiled coil:
Domain [FT]: DOMAIN 2 289 Thymidylat_synt. {ECO:0000259|Pfam:PF00303}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99373
UniPathway: K00560
CDD:
Gene3D:
HAMAP: 3.30.572.10
InterPro: MF_00008
PANTHER: IPR023451;IPR000398;IPR020940
PIRSF:
PRINTS:
PROSITE: PR00108
Pfam: PS00091
ProDom: PF00303
SMART:
SUPFAM:
TIGRFAMs: SSF55831
63126-63996(+)

>nucleotide sequence
ATGAAAACATACTTAGATTTGTTAGAAAAAGTTATGACCAAAGGTGAGGTTAAAAAAGAT
CGAACTAACACGGGTACGATCTCTTATTTTGGTACTCAAAGTCGATTTGATTTACGTGAA
GGATTTCCATTATTAACAACTAAGAAAATGGCTTGAAAAGCAATTTGCCATGAACTATTA
TGATTTCTTAAAGGTGATACGAATATTAAGTACTTAGTTGATAATAATGTGAACATTTGA
AACGAATGGCCTTATGAAGCTTTTAAGAAATCAAATGACTTTAAAGGAGAGAGCTTACAA
TACTTCGTTGAGAAAATTAAAACTGATAACCAATTTGCTCAACAATATGGAAAATTAGGA
CCTGTTTATGGAAAACAATGACGTGACTTTAATGGCGTTGATCAAATTGAGTGAGTTATC
AACGAAATTAAAACTAATCCGTTTTCACGTCGTTTAATAGTAAATGCGTGAAATCCTGGT
GAAATAAAAGAGATGGCCCTACCACCTTGTCATAGTTTTTTCCAGTTCTTTGTTAATGAA
AAATATGAAATTTCACTGCAACTATACCAAAGAAGTGGGGACCTATTCTTAGGCGTGCCT
TTTAATATTGCTTCTTATAGTTTGTTACTTTTAATGGTTGCTCAAGTAACCGGGGTAAAA
CCTGCTGAATTTATCCATACAATTGGGGATAACCACATCTATTCAAACCACCTTGAACAA
GTAAAAATTCAAATACAAAGAGAACCTAAAAAACTTCCTAAAGTAACCCTAAACCCTGAT
ATTAAAAATATCTTTGATTTTAAATTCGAAGATATCAGTTTAGAAAACTATGAACACCAT
GACCCAATCAAAGGAAAGGTAGCTGTTTAA

>protein sequence
MKTYLDLLEKVMTKGEVKKDRTNTGTISYFGTQSRFDLREGFPLLTTKKMAWKAICHELL
WFLKGDTNIKYLVDNNVNIWNEWPYEAFKKSNDFKGESLQYFVEKIKTDNQFAQQYGKLG
PVYGKQWRDFNGVDQIEWVINEIKTNPFSRRLIVNAWNPGEIKEMALPPCHSFFQFFVNE
KYEISLQLYQRSGDLFLGVPFNIASYSLLLLMVAQVTGVKPAEFIHTIGDNHIYSNHLEQ
VKIQIQREPKKLPKVTLNPDIKNIFDFKFEDISLENYEHHDPIKGKVAV