GCW_00410

Uniprot: A0A0F6CJX8
Description: lysyl-tRNA synthetase
EC number: 6.1.1.6
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). {ECO:0000256|HAMAP-Rule:MF_00252, ECO:0000256|RuleBase:RU000336, ECO:0000256|SAAS:SAAS00105435}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00252, ECO:0000256|RuleBase:RU000336}; ; Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000256|HAMAP-Rule:MF_00252, ECO:0000256|RuleBase:RU000336}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 403 403 Magnesium 1. {ECO:0000256|HAMAP-Rule:MF_00252}.; METAL 410 410 Magnesium 1. {ECO:0000256|HAMAP-Rule:MF_00252}.; METAL 410 410 Magnesium 2. {ECO:0000256|HAMAP-Rule:MF_00252}.
Nucleotide binding:
Site:
Gene names (primary): lysS
Gene names (synonym):
Mass: 57,343
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00252}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; lysine-tRNA ligase activity [GO:0004824]; magnesium ion binding [GO:0000287]; nucleic acid binding [GO:0003676]; lysyl-tRNA aminoacylation [GO:0006430]
Gene ontology IDs: GO:0000287; GO:0003676; GO:0004824; GO:0005524; GO:0005737; GO:0006430
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00252, ECO:0000256|SAAS:SAAS00546954}.
Protein families: Class-II aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]: DOMAIN 173 487 AA_TRNA_LIGASE_II. {ECO:0000259|PROSITE:PS50862}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99400
UniPathway: K04567
CDD:
Gene3D: cd00775
HAMAP: 2.40.50.140
InterPro: MF_00252
PANTHER: IPR004364;IPR018150;IPR006195;IPR002313;IPR018149;IPR012340;IPR004365
PIRSF: PTHR22594
PRINTS:
PROSITE: PR00982
Pfam: PS50862
ProDom: PF00152;PF01336
SMART:
SUPFAM:
TIGRFAMs: SSF50249
88385-89864(+)

>nucleotide sequence
ATGTCTAAAAAACTTAACGATCAGGAACTGGTTCGTTTAGATAAACTAAACCGTTTAATT
GAATCTAAACAAAACCCATACGAGGTTACAAAAGTTGCTAACACTCACAACACAAAAAGC
TTAAAAGAAAAATACGACCAATTTTCTAAAGAACAGTTAGCAGAGATGCAACTGGATAAG
CCAATTACTGTTAGTGGGCGTGTAATCTTGATTAGAAGAACATTTATTCTAATTCAAGAT
TTTCATTCAGAATTACAACTATATATAAATAAAAATAAACAACCTGATCTGTTTAAGTAT
TTCAATGACTATTTAGATTTAGGTGATGTCGTTTGTGCTACTGGTAAACCAATGAAAACC
AATACTAATGAACTTTCATTAGATCTGGAATCATTAAAGATTATCAGTAAATCTTTAAGA
GTACCACCTGAAAAATTCCACGGGATCGCTGATGAAGAGATCCGTTCACGAAAACGTTAT
TTAGACCTAGTTTATAATAAGGAATCAAAAGAAAGATTCGTTTATCGTTCAAAGATTATT
GCAGCAATGCGCCAATATTTTAATGAAAACGGTTTTTTAGAAGTAGAAACACCATTTTTA
CATGCCCAGATCGGAGGAGCTGCTGCTAAGCCTTTTATAACAAGATATAATGCACTTGAT
CGTGATTATTATTTAAGAATTGCTCCTGAATTACCTCTTAAAAAACTTATCGTTGGAAGT
TTTGAAAAGATATATGAGATCGGTAAGTGTTTTAGAAACGAAGGGATGGATTCAACTCAC
AACCCTGAATTTACCAGTGTTGAAACTTACGTAGCTTATGTTGATTATATCTACATGATG
GAACTAACCGAGGCTCTAATTAAGTATATTGCCAAAACAATTGGTATTAGTCACACTAAT
ATCAAGAATGAAACGGTTGATTGGAATAAACCATTCAAACGAATTAAGATGACTGAATTA
GTTAAACAAGAAACTGGGATCGATTTTACGCAAGTAAAAAAATTAGATGAAGCGTTAGAT
TTAGCTAAAAAACATAAGGTTCATGTTAAAGAACATGAAAAGACAATAGGTCATATTATC
AATTTATTTTTCGAGGAATTTTGCGAGAAAAAATTGGTTGAACCAACGTTTGTAACTCAC
CATCCAGTAGAGATCTCACCGTTATCAAAATTAGATTATTCAGATCCAAGATATACTGAA
AGATTCGAACTATTTGCGTTTGGTAAAGAATTATCTAACGGGTTTAGCGAATTAAACGAT
CCAATCGATCAAAGACAACGATTTGAAAAGCAATTAGAAGAAAAACAAAAGGGTAATGAT
GAAGCTTCTGAAATGGATGAAGATTTCCTAGAAGCTCTAGAAAATGGCCTGCCACCAACT
GGTGGTTTGGGCATTGGAGTAGACCGTTTAGTGATGATGTTAACAGGCACAACATCAATC
AGAGATATCTTGTTTTTTCCGCACGTACGTGAAGAATAA

>protein sequence
MSKKLNDQELVRLDKLNRLIESKQNPYEVTKVANTHNTKSLKEKYDQFSKEQLAEMQLDK
PITVSGRVILIRRTFILIQDFHSELQLYINKNKQPDLFKYFNDYLDLGDVVCATGKPMKT
NTNELSLDLESLKIISKSLRVPPEKFHGIADEEIRSRKRYLDLVYNKESKERFVYRSKII
AAMRQYFNENGFLEVETPFLHAQIGGAAAKPFITRYNALDRDYYLRIAPELPLKKLIVGS
FEKIYEIGKCFRNEGMDSTHNPEFTSVETYVAYVDYIYMMELTEALIKYIAKTIGISHTN
IKNETVDWNKPFKRIKMTELVKQETGIDFTQVKKLDEALDLAKKHKVHVKEHEKTIGHII
NLFFEEFCEKKLVEPTFVTHHPVEISPLSKLDYSDPRYTERFELFAFGKELSNGFSELND
PIDQRQRFEKQLEEKQKGNDEASEMDEDFLEALENGLPPTGGLGIGVDRLVMMLTGTTSI
RDILFFPHVREE