GCW_00670

Uniprot: A0A0F6CK17
Description: alanyl-tRNA synthetase
EC number: 6.1.1.7
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). {ECO:0000256|HAMAP-Rule:MF_00036, ECO:0000256|SAAS:SAAS00015802}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_00036}; ; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00036}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain. {ECO:0000256|HAMAP-Rule:MF_00036, ECO:0000256|SAAS:SAAS00345671}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 567 567 Zinc. {ECO:0000256|HAMAP-Rule:MF_00036}.; METAL 571 571 Zinc. {ECO:0000256|HAMAP-Rule:MF_00036}.; METAL 671 671 Zinc. {ECO:0000256|HAMAP-Rule:MF_00036}.; METAL 675 675 Zinc. {ECO:0000256|HAMAP-Rule:MF_00036}.
Nucleotide binding:
Site:
Gene names (primary): alaS
Gene names (synonym):
Mass: 104,524
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; alanine-tRNA ligase activity [GO:0004813]; ATP binding [GO:0005524]; tRNA binding [GO:0000049]; zinc ion binding [GO:0008270]; alanyl-tRNA aminoacylation [GO:0006419]
Gene ontology IDs: GO:0000049; GO:0004813; GO:0005524; GO:0005737; GO:0006419; GO:0008270
Chain:
Signal peptide:
Domain [CC]: DOMAIN: Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. {ECO:0000256|HAMAP-Rule:MF_00036}.
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00036, ECO:0000256|SAAS:SAAS00541271}.
Protein families: Class-II aminoacyl-tRNA synthetase family
Coiled coil: COILED 755 785 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 5 714 AA_TRNA_LIGASE_II_ALA. {ECO:0000259|PROSITE:PS50860}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99439
UniPathway: K01872
CDD:
Gene3D:
HAMAP:
InterPro: MF_00036_B
PANTHER: IPR002318;IPR018162;IPR018165;IPR018164;IPR023033;IPR003156;IPR018163;IPR009000;IPR012947
PIRSF:
PRINTS:
PROSITE: PR00980
Pfam: PS50860
ProDom: PF02272;PF01411;PF07973
SMART:
SUPFAM: SM00863
TIGRFAMs: SSF101353;SSF50447;SSF55186
161425-164134(+)

>nucleotide sequence
ATGAATAAACACTTAAGTGGCTCGCAAATAAGACAAATCTGGTTAGATTTCTTTAAATCA
AAAAAACATGAGATCATTGAATCAAAATCATTAGTACCTATCAATGATCCTAGTTTATTG
TGAATAAATGCAGGTGTAGCAACTTTAAAAAAATACTTTTCAGGTGAAGAAAACCCCACC
AATCCAAGATTAGCTAATTCGCAAAGATGTATTAGAGCTAACGATATTGAAAACGTTGGA
GTAACATCAAGACACCATACTGTTTTTGAAATGCTTGGAAACTTTTCAATCGGGGATTAC
TTCAAAAAGGAAGCAATCGAATTTGGTTATGAATTATTAACCAAATTCTATGGTTTGAAT
AAAGATAAGCTATACATCACAATTTATGAAGATGATAACGACGCTTTTAATTATTGAGTA
GATCAAGGGATCAATCCTCACCACATCATCAGATGTGATAAGAAAAGAAATTTCTGAGAT
CTAGGATCTGGTCCTTGTGGTCCAAGTACAGAAATTTATTACGATCGAGGTGAAAAATAC
GACCCTAATAAATTAGGTGAAAAACTTTTCTTTGAAGATATTGAAAATGATCGTTATATC
GAAGTATGAAATATCGTTTTTTCTCAATTTAACAATACTGGAAATAATAACTACACAGAA
TTATTAAGAAAAAATATTGATACAGGTGCTAGTTTAGAACGATTTGCATGTATTTTACAA
GACGTTCCAACTAATTATGACACTGATCTATATTTACCAATAATCAGAACAATTGAAAAA
CACACTAATCATAAATACGTTGTTGATCATTACTTTAGTAAAGATCAATCTGAACAAGAT
GTTTTAAGAGCCTTCCGTGTTATTGCTGACCACTTAAAGTGTGGTGTGTTTGCAATCGCT
GATGGTGTGTTGCCTGGTGCTAAAGATCGTGATTACATTATTAGAAAGCTACTACGTAGA
GCTTTTGTATATGCTAAAAAGTTAAATGCTAAACCAGAATATCTGGTTGAAACAATTAAT
GTAATCATTAACACCTATAGCGATTTCTTTAAATATTTAATTCCAAATAAAGAAATTGTT
ATTCAAGCAATTACTAATGAAGCAAATAATTTTACTAAAACATTAGATTATGGTTTTGAG
ATCTTTAATGAAGCAAAAACAACTAATAATATAACTGCTGAAACGATCTTTAAACTAGTT
GAAACCTATGGTTTCCCTTTAGATTTAATTAAAGAATTATCAGCTGAAGCAAAGATCAAA
TTAGATTTAGATGGTTTTGAGGAACTATTTAAAAAACACCAAGAAATTTCAAAAGCTAAT
AATGGTGAAGTTGGTCTAAAAAAACAAAATGAAAACTTGCTTAAGTTTGAGACACCATCT
AAGTTTTTCTATGACAAAAATAATATAAAAACTAAAGTGGTTGCAATCTTTGATGATAAG
TTTAACCCAGTAGACCAGATTGAAGTTGGTTCTGGTTGAGCGGTTTTTGAAAGCACACCG
ATCTATGCCACATCTGGTGGTCAAAGATTTGACGAAGGTTATTGTCTTAAAAAAGGCAAT
CTTGTTGTTCATTTTGATAATGTCATTAAAGCTCCAAATAAGCAACACCTACACCACTTT
AAGAAGGCAAGTTTTTGGCTTAACGAAAAAGTGGAGTTAATGCATGATGAGAACTGAAGA
AAACTAGTAAGAAAAAACCACTCTTTAGAACATATCTTACACGCGACACTTAAAAATACG
ATAAGTGAAACAATTAAACAATCAGGTGCATTTAAATCAGCAGCTAAAGCAACTCTAGAC
TTTAATTACCCAACAAGATTAACAGATGAAGATCTGGATAAAATTGAAACCAAAATGCGC
CAGGTAATCGCAGATAAAATTCCAGTTACCGTGCACCATGTTGACTATGAAACTAGTCAA
AAAATGAATGCAATTGCTTACTTTGAAGAAGAATATAAAAAACACGAATTACTTCGAGTA
ATCAAGATTGGTGATTACAGTGTTGAACTGTGTGGCGGAACTCATGTTGACAACACCAAA
GAGATTGAAGAATGTTTTATTACTAACCTTTATTCATTAGGTGCTGGTCGTTGAAGAATT
GAAATCATGAGTTCTTATGAAACGATTTACAACTATTTAGTTGATAAACAAAACGAGATC
AAACAAGAAAAAGATCATATGTTCAATGAATTAGGTAATTACGATCTAACTCATTACTTA
GATTTTAGTAAGAAATTACATTCATTTAATTTACCTAATTCTATTAAAGATCTAAGAAAG
ACTTTAAGAGCGTTTGAACAGATTAAAGAAGAATATAAGGCCATTAAATTAGAGTTAGAT
AAAAAGAAAACCAAAGACCGTGCTAACCAGATTAAAAAATTGGCTTTAGACAACTTAGAA
CATAAAATTGTTTTATTGTTCTTTGATAAAGAAGAACATAAAGCTTTATCAATTGCTCAT
AGTGAATTAGTTAATGAAAAACCAGATCATCTCTTCTTTTTCATTAATAAATCTGATAAT
AAGATTAACTATCTAATCGGAATTAAGAATGCCACTGATAAGTTGAATGCTAAGCTATTA
ATTGATAAAGTTAATGCGATGTTTGGTGCTAAAGGTGGTGGTAAGCAAAACTTTGCTCAA
GGTGGATTTAGCACAGATAAAGATGTTGGTAAACTAAAAGCAGATTTCGTTAATATATGT
ATTACGTAG

>protein sequence
MNKHLSGSQIRQIWLDFFKSKKHEIIESKSLVPINDPSLLWINAGVATLKKYFSGEENPT
NPRLANSQRCIRANDIENVGVTSRHHTVFEMLGNFSIGDYFKKEAIEFGYELLTKFYGLN
KDKLYITIYEDDNDAFNYWVDQGINPHHIIRCDKKRNFWDLGSGPCGPSTEIYYDRGEKY
DPNKLGEKLFFEDIENDRYIEVWNIVFSQFNNTGNNNYTELLRKNIDTGASLERFACILQ
DVPTNYDTDLYLPIIRTIEKHTNHKYVVDHYFSKDQSEQDVLRAFRVIADHLKCGVFAIA
DGVLPGAKDRDYIIRKLLRRAFVYAKKLNAKPEYLVETINVIINTYSDFFKYLIPNKEIV
IQAITNEANNFTKTLDYGFEIFNEAKTTNNITAETIFKLVETYGFPLDLIKELSAEAKIK
LDLDGFEELFKKHQEISKANNGEVGLKKQNENLLKFETPSKFFYDKNNIKTKVVAIFDDK
FNPVDQIEVGSGWAVFESTPIYATSGGQRFDEGYCLKKGNLVVHFDNVIKAPNKQHLHHF
KKASFWLNEKVELMHDENWRKLVRKNHSLEHILHATLKNTISETIKQSGAFKSAAKATLD
FNYPTRLTDEDLDKIETKMRQVIADKIPVTVHHVDYETSQKMNAIAYFEEEYKKHELLRV
IKIGDYSVELCGGTHVDNTKEIEECFITNLYSLGAGRWRIEIMSSYETIYNYLVDKQNEI
KQEKDHMFNELGNYDLTHYLDFSKKLHSFNLPNSIKDLRKTLRAFEQIKEEYKAIKLELD
KKKTKDRANQIKKLALDNLEHKIVLLFFDKEEHKALSIAHSELVNEKPDHLFFFINKSDN
KINYLIGIKNATDKLNAKLLIDKVNAMFGAKGGGKQNFAQGGFSTDKDVGKLKADFVNIC
IT