GCW_00805

Uniprot: A0A0F6CK36
Description: Holliday junction resolvase
EC number: 3.1.22.-
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00130}; ; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00130}
Enzyme regulation:
Function [CC]: FUNCTION: Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation. {ECO:0000256|HAMAP-Rule:MF_00130, ECO:0000256|SAAS:SAAS00368725}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 56 56 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00130}.; METAL 69 69 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00130}.; METAL 87 87 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00130}.
Nucleotide binding:
Site: SITE 71 71 Transition state stabilizer. {ECO:0000256|HAMAP-Rule:MF_00130}.
Gene names (primary): recU
Gene names (synonym):
Mass: 20,687
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; endonuclease activity [GO:0004519]; magnesium ion binding [GO:0000287]; nucleic acid binding [GO:0003676]; chromosome segregation [GO:0007059]; DNA recombination [GO:0006310]; DNA repair [GO:0006281]
Gene ontology IDs: GO:0000287; GO:0003676; GO:0004519; GO:0005737; GO:0006281; GO:0006310; GO:0007059
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RecU family. {ECO:0000256|HAMAP-Rule:MF_00130, ECO:0000256|SAAS:SAAS00558495}.
Protein families: RecU family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP006916
ProteinModelPortal: A0A0F6CK36
MEROPS:
EnsemblBacteria KO: AHB99458
UniPathway: K03700
CDD:
Gene3D:
HAMAP: 3.40.1350.10
InterPro: MF_00130
PANTHER: IPR004612;IPR011335;IPR011856
PIRSF:
PRINTS: PIRSF037785
PROSITE:
Pfam:
ProDom: PF03838
SMART:
SUPFAM:
TIGRFAMs: SSF52980
184284-184809(+)

>nucleotide sequence
ATGCATCAAAATAGAGGAATGTTTCTTGAAACATTAATCAATAATACAATTAAGCATAAC
GAATTAGCCCACAAAGGGCTAATTTTTAAACGTCACTTACCAATCAATGTTTATAATTTT
GCTAACAGAAGAGTTGCTGGGTGATTAAAAGAAAAGACACAAACCGATTATTATGGGCTT
TATAAGGGTCATTTCTTTGATTTCGATGCAAAACAAAGCTCTAAAATTAATTATTCGCTT
AAGAATATCAAGCAACATCAACTAGATCATTTAAGAAAGATTCACCAACAAGGAGGGATT
GCTTTTATCTTATTGTTAATCGTTCCCAAAGAAGAGTTTTATATGATTCCAATTGAAAAA
ATTGATTTTTGGTTAAAAAACCAAGAATCAAACACCTTAAAGTATGAATGGATTCAGAAA
AGCTCTTTTAAATTGGAACTATTTTATCCCGGAGTAATTGGGATCTTTGAAGCTCTTCAA
GAATGGATCGACTTAATTACTCTTCGTCGTTCTGGCGGTTCTTAA

>protein sequence
MHQNRGMFLETLINNTIKHNELAHKGLIFKRHLPINVYNFANRRVAGWLKEKTQTDYYGL
YKGHFFDFDAKQSSKINYSLKNIKQHQLDHLRKIHQQGGIAFILLLIVPKEEFYMIPIEK
IDFWLKNQESNTLKYEWIQKSSFKLELFYPGVIGIFEALQEWIDLITLRRSGGS