GCW_01080

Uniprot: A0A0F6CK76
Description: CTP synthetase
EC number: 6.3.4.2
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate. {ECO:0000256|SAAS:SAAS00037321}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. {ECO:0000256|SAAS:SAAS00037305}.
Pathway: PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. {ECO:0000256|SAAS:SAAS00037318}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): pyrG
Gene names (synonym):
Mass: 61,282
Subunit structure [CC]: SUBUNIT: Homotetramer. {ECO:0000256|SAAS:SAAS00356542}.
Gene ontology (GO): ATP binding [GO:0005524]; CTP synthase activity [GO:0003883]; 'de novo' CTP biosynthetic process [GO:0044210]; glutamine metabolic process [GO:0006541]
Gene ontology IDs: GO:0003883; GO:0005524; GO:0006541; GO:0044210
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the CTP synthase family. {ECO:0000256|SAAS:SAAS00548960}.; SIMILARITY: Contains glutamine amidotransferase type-1 domain. {ECO:0000256|SAAS:SAAS00037304}.
Protein families: CTP synthase family
Coiled coil:
Domain [FT]: DOMAIN 300 540 Glutamine amidotransferase type-1. {ECO:0000259|PROSITE:PS51273}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99498
UniPathway: K01937
CDD:
Gene3D:
HAMAP: 3.40.50.300;3.40.50.880
InterPro:
PANTHER: IPR029062;IPR004468;IPR017456;IPR017926;IPR027417
PIRSF: PTHR11550
PRINTS:
PROSITE:
Pfam: PS51273
ProDom: PF06418;PF00117
SMART:
SUPFAM:
TIGRFAMs: SSF52317;SSF52540
243985-245608(+)

>nucleotide sequence
ATGACTAGTAAAAAGACTAAATATATCTTTGTTTCTGGAGGAGTTTATTCTTCAGTGGGC
AAGGGGTTGATCGTTGCTTCGATTGCAAGTATTTTCAAACACCAAAACTTTAAGATTAAT
ATCTTAAAGCTTGATCCCTATCTTAATGTTGATTCGGGAACGATGTCACCATATGAACAT
GGTGAGGTGTTTGTTACTTCGGATGGAACAGAGACTGATTTAGATCTTGGTTATTATGAA
CGTTTCTTAGTTCAAGATCTAAATGAACGTTCATCAATCACATCAGGTAAAGTTTATTTT
AGTGTGATTAATAAAGAACGTAAGGGTGAGTATTTGGGTAAAACGGTCCAATTAATCCCT
CACGTTACTAATGAGATTAAAAGACGGATTGCTCTAGCAGCTACTGATGAGAACAATCAA
TCTTACGATTTAGTTTTTTGTGAGATCGGTGGGACGATTGGTGATTTAGAATCATTACCT
TTTATTGAGGCAATCAAGCAGATCATTCGCGAAAATAATAAGCACGACACGGCTTTTATC
CATGTGGTGCCCATCATTAGTTTATCTAACAGCGAACAGAAAACCAAACCATTACAACAC
TCTTTAAAAGAGTTAAACAACCTTGGGATTAATCCCGATTTTTTAGTAATTAGATCTAAA
GAAAGTTTAGATCTAAAAACCAAGCATAAGATTGCTAGTGCAACCTATCTAAATAAAGAT
CATATCTTTAACTCTGTTGATTTAAATAATACTTACTTCTTACCAGATTATTTAAATCAA
CAAAAGATCCATGAAAAGATTGCTAACAAATTACAGATCACGATTGATCCAAAGATTAAG
TTATCAGCTTGAGATGATCTAGCTAAGAAGATTAATAACCAAGATAACTTAGTAAGAAAG
ATCGCGATTATTGGGAAATATACCCAATTTAAAGATGCATATCTCTCTTTAATTGAATCG
CTTAATTTAGCTGGTTATCATAACCACGTTAAGTTAGAGATCGATTGAATCGAATCATCA
TTATATGATGATGCATCTTTAGAAGAAGTTAATCATAAACTTAAAGATGTTCATGGGGTT
ATTGTCCCTGGTGGGTTTGGTTCTAGGGGTGTTGAAGGTAAGATTAAGTTCATTCAGGTT
GCTAGAACTAAAAAGATTCCATTCCTTGGGATCTGTTTGGGGATGCAATTAGCTTGTGTT
GAGTATGCTAGAAATGTGTTGGGAATGGCTGATGCTAACTCAAGTGAGTTTAATTCACAA
ACTACTTATCCGATCTTTAAGATAATGAACCCAGATGATCTTAATTATGGTGGAAGTTTA
AGATTGGGTGATTATTTAGTTAGTTTAAAAGATCAAACTTTAGCTAAAGCTATTTATCAA
CAAGATAGTATTAAAAGAAGACACCGTCACCGTTATGAGTTTAATAACGATTATTTAAAC
CAGTTTAATGACGGTGAATTCATTGTTTCAGGGATCTATCTTCAAGAAAACTTAGTCGAA
ACAATCGAACTAAAAAACCATCCGTTTTTTATTGGTTGTCAGTATCACCCAGAGTTTTCA
TCAAAGATTACAGCGGCTGAAGCGTTGTTTGATTCACTAGTTAAAAAAATCAAAATGTTA
TAA

>protein sequence
MTSKKTKYIFVSGGVYSSVGKGLIVASIASIFKHQNFKINILKLDPYLNVDSGTMSPYEH
GEVFVTSDGTETDLDLGYYERFLVQDLNERSSITSGKVYFSVINKERKGEYLGKTVQLIP
HVTNEIKRRIALAATDENNQSYDLVFCEIGGTIGDLESLPFIEAIKQIIRENNKHDTAFI
HVVPIISLSNSEQKTKPLQHSLKELNNLGINPDFLVIRSKESLDLKTKHKIASATYLNKD
HIFNSVDLNNTYFLPDYLNQQKIHEKIANKLQITIDPKIKLSAWDDLAKKINNQDNLVRK
IAIIGKYTQFKDAYLSLIESLNLAGYHNHVKLEIDWIESSLYDDASLEEVNHKLKDVHGV
IVPGGFGSRGVEGKIKFIQVARTKKIPFLGICLGMQLACVEYARNVLGMADANSSEFNSQ
TTYPIFKIMNPDDLNYGGSLRLGDYLVSLKDQTLAKAIYQQDSIKRRHRHRYEFNNDYLN
QFNDGEFIVSGIYLQENLVETIELKNHPFFIGCQYHPEFSSKITAAEALFDSLVKKIKML