GCW_01105

Uniprot: A0A0F6CK81
Description: transcription elongation factor GreA
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. {ECO:0000256|HAMAP-Rule:MF_00105, ECO:0000256|RuleBase:RU000556, ECO:0000256|SAAS:SAAS00338401}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): greA
Gene names (synonym):
Mass: 17,944
Subunit structure [CC]:
Gene ontology (GO): DNA binding [GO:0003677]; translation elongation factor activity [GO:0003746]; regulation of DNA-templated transcription, elongation [GO:0032784]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003746; GO:0006351; GO:0032784
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the GreA/GreB family. {ECO:0000256|HAMAP-Rule:MF_00105, ECO:0000256|RuleBase:RU000556, ECO:0000256|SAAS:SAAS00541981}.
Protein families: GreA/GreB family
Coiled coil:
Domain [FT]: DOMAIN 8 77 GreA_GreB_N. {ECO:0000259|Pfam:PF03449}.; DOMAIN 85 157 GreA_GreB. {ECO:0000259|Pfam:PF01272}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal: A0A0F6CK81
MEROPS:
EnsemblBacteria KO: AHB99503
UniPathway: K03624
CDD:
Gene3D:
HAMAP: 1.10.287.180;3.10.50.30
InterPro: MF_00105
PANTHER: IPR018151;IPR006359;IPR028624;IPR001437;IPR023459;IPR022691
PIRSF:
PRINTS: PIRSF006092
PROSITE:
Pfam: PS00829
ProDom: PF01272;PF03449
SMART:
SUPFAM:
TIGRFAMs: SSF46557
250561-251041(+)

>nucleotide sequence
ATGGCAAAAACAAAAAAACACTTATTAACCCAAGAAGGGTTAAAGAAACTGCAAGCAGAA
CTAAGACTTTTAGTTGATGTTAAACGTGCAGATGTAATTAAATTAATTCAAGAAGCACGT
GAGCAAGGTGACTTATCTGAAAATGCTGATTATGATTCAGCTAAAGCAACTCAAAGCGAG
ATCGAAAGTCGGATTACTGAGATTCAAGATATCTTAAATCACTATGAGATCATCAAAGAA
GTTGATACGAAAAACAAACGGGTAATCGTTGGTGCTAAAGTAACGATCCACGATCATTCA
GATGAATGTAATTATACGTATGAGATTGTGGGTCCAATTGAAAGTGATCCAGCTGAAAAT
AAGATCTCACACGAATCACCTGTAGCTAAAGCGATCATGGATAAAAAAGAAGGTGAATCA
GCTGAAGTGATCGGGATTGAACACCCTTATAAAGTAACGATTAAAAAGATCGTGCTTTAG

>protein sequence
MAKTKKHLLTQEGLKKLQAELRLLVDVKRADVIKLIQEAREQGDLSENADYDSAKATQSE
IESRITEIQDILNHYEIIKEVDTKNKRVIVGAKVTIHDHSDECNYTYEIVGPIESDPAEN
KISHESPVAKAIMDKKEGESAEVIGIEHPYKVTIKKIVL