GCW_01240

Uniprot: A0A0F6CK97
Description: DNA-directed RNA polymerase subunit beta
EC number: 2.7.7.6
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoB
Gene names (synonym):
Mass: 156,035
Subunit structure [CC]: SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
Gene ontology (GO): DNA binding [GO:0003677]; DNA-directed RNA polymerase activity [GO:0003899]; ribonucleoside binding [GO:0032549]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003899; GO:0006351; GO:0032549
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA polymerase beta chain family. {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
Protein families: RNA polymerase beta chain family
Coiled coil:
Domain [FT]: DOMAIN 33 552 RNA_pol_Rpb2_1. {ECO:0000259|Pfam:PF04563}.; DOMAIN 477 523 RNA_pol_Rpb2_2. {ECO:0000259|Pfam:PF04561}.; DOMAIN 604 672 RNA_pol_Rpb2_3. {ECO:0000259|Pfam:PF04565}.; DOMAIN 682 749 RNA_pol_Rpb2_45. {ECO:0000259|Pfam:PF10385}.; DOMAIN 811 1273 RNA_pol_Rpb2_6. {ECO:0000259|Pfam:PF00562}.; DOMAIN 1275 1349 RNA_pol_Rpb2_7. {ECO:0000259|Pfam:PF04560}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99519
UniPathway: K03043
CDD:
Gene3D: cd00653
HAMAP: 2.30.150.10;2.40.270.10;2.40.50.150;3.90.1110.10
InterPro: MF_01321
PANTHER: IPR019462;IPR015712;IPR007120;IPR010243;IPR007121;IPR007644;IPR007642;IPR007645;IPR007641;IPR014724
PIRSF: PTHR20856
PRINTS:
PROSITE:
Pfam: PS01166
ProDom: PF04563;PF04561;PF04565;PF10385;PF00562;PF04560
SMART:
SUPFAM:
TIGRFAMs:

In one protein complex with GCW_01245 GCW_03530 283131-287304(+)

>nucleotide sequence
GTGAAAAAAAACTCGGCCTTTCATAATACTCAATATTCACCAAAAGTAGTTAGAAGAGAC
TATTCAAAAGTTCATAACAAATATAACCCCTTAAATTTACCAGGGATTCAAGTAAACGCT
TATAAGCAGTTTCTTGAAAAAGAATTAGAAGAGATCATCGGATCATACTTCCCAATCAAA
TCACCAAACGGAAAATATTCTGTGGAATTCCACGGAATGAAGATCCTTGATCCACAGATC
ACAAAAGAAGAAGCTAGTGCAGAATCAAAAACCTATGACGCTAGCTTATATGTTAATTTA
TCTTTAGTTAACCACCAAACTGGGACAGTAAAAAAGATTAGTAAAAAATCAGCTAAATCA
AAGGCTGAAGGGATCTATTTTGCTGATATTCCATTGATGACCGAAAACGGAGCTTTCATT
GTTAATGGGATTGAAAAGTTTGTTGTTGCTCAGATCGTGAGATCACCAGGTGCTTACATT
CTAAATAAGTCACAAGTTAAACTATCTAACTCAAGAAAACGTAACCAAGAAGGTTATATC
TGTGAAGTATTCCCTTCTAAGGGAACATTAATGTTGTTCTATATTGCTGAAAACAAAGAC
TTTGTTCAAGCAGTTGTGCGTGATGTTGGTGGTGAAAGTGCTAAGGTATTTTCAATTACG
ACATTGCTTAAAGCCTTTGGTTTAAGTGAGATTAAGATTAAAGAGATCTTTAATAATAAT
GATTACATCTTAAGATCATTAGAATCTGAGTTTTATAATGAAAAACAAATTCTTAATGAA
GCTGATATCGCGCAATTAATCCGCGATATCGAATCAGACCGCATTAGCAAAGTTAAATCT
TTACCGATTGATCAAAAATGAAAGAACTTAGTTTTAGATTGATACAAACTGAACCAAGAA
AAGCAAGAACTACTAAACTCATCTAACCCAAATCCAACTAAGATCGAGTCTTTAAACACC
CACATCGGTGTTGTTTTAAGAAAACTAATCTGTGAAAAGGCTGCTAAACACGTAATTCAA
GAACTATCAATCTCAACAAGAAGTCTTGATAACGTTTCACAAAAAGAAGAGATCTCTTAC
CAATCAATCTTATTACAACACTTCTTCCAAAAGAAACGTTATGACCTATCTTCAGCAGGT
CGTCACAAATTCGTTCGTAAACTAAGAGTTTCTGAACGTTTATACCAACGAACAATTGCT
CAAGATATTAAAGATCTTGATGGTAATGTTGTAATTAAGCAAGGGACGTTAATGCTTAAA
GAACAACTTGATTTATTTAAACGTTTATCAAAAGAAAAACGTTTAGACATCATCAAGCAG
ATCGATTTCATTAACCCAGAACTAAATACGCAATTTACTGATGTAAATACGTATGAAGAA
GTTCAGATCTATGTTAATAATGATCTAAGAAATGAAACGACCCAAATTATTGGGATCGAT
GGATCTAATGAATCGATTGAAACTTTAACTTTAGCTGATCTAATTTCAATCGTTTCATAT
ATCGTTAACTTACCATTCAATATCGGTTTATATGACGATATTGATCATTTAGGTAATAAG
CGTTTAAAACTAATTAACGAATTATTAAAATCTAAAGTTCAAACCGGAATGATGCGGATT
GAAAAATACATCAAAGATAAGTTACAAACTGCAGATGGTAATAATAAGTTAGCTGATCAA
GATGCTGAAAACGATCCTGATTCAGATCTTGGTACTAAGAGTAGTAATGATCTAACTGTG
AAATCAGTTATTAATCCAAAACCATTCCAAATCGTGATTAAAGATTTCTTTAACACGCAC
CAATTAACCCAATTCTTAGATCAACAAAACCCATTATCTGAATTAACTAATAAGAGAAGA
ATTTCAGCAATGGGTCCTGGGGGGATTTCACGGGAAGACCCTAACTTAGATATCCGTGAC
GTTCACTATTCTCACTATGGAAGAATCTGTCCGATTGAAACCCCTGAAGGGATGAACATC
GGTTTGATTATGTCATTAGCGTTTTATGCGACCATTGATAAGAATGGGTTCTTAATGACC
CCTTATCTAAAAGTAGAAAACGGTAAGATTACAGATAAAGTTGAACACTTAACTGCACTA
AGAGAAGATGAATATATTATTGCAGAAGCTTCTTCTTATATGGATGTTGCTAAAGATGGA
ACGATTAATAACGAAAAGATTATTGCTAGATATCGTTCATCTCAAGATCTATATGACCCT
AAGCAAGTTGATTATATCGACGTTTCACCAAAACAAGTTGTTTCAGTAGCAGCTTCATTA
ATTCCGTTTTTAGAAAACGATGACTCGTCTCGGGCTTTGATGGGTGCAAACATGCAACGT
CAAGCTACACCTTTACTAAAACCTTACTCTCCATTAGTTGGGACTGGTGTTGAGTATAAG
ATTGCTCAAGACTCAGGGATGTTAGTAACAGCTAAAAACCCTGGAACAGTTACTTATGTT
GATTCTAGTAAGATCACTGTTAAGGGTGAAGATAATAAATCAACTGATTACAACTTATTA
AAGTTTGTTAAATCAAATAAGAACACTTGTTATAATCAATCACCGATCGTTGCTGTTGGT
GATAAGATTGAAACTAACCAAGCTTTAGCTGATGGTCCATCAATGAAAAACGGTGAGCTC
GCACTAGGACAAAACGTTCTAGTTGGTTTTACTACTTGAAGTGGTTATAACTACGAAGAC
GCAGTGATTATCTCTGACCGTTTATTTAAGGACGATGTTTATTCATCAATTCATATCGAT
GAATACACAATCGAATGTTTAAGAACTAAAAACGGTGATGAAGAGATTACTAGAGATATT
CCAAATATCTCAGAATCAGCTAAGCGCTACCTTGATGAAGAAGGTGTAATTATGGTTGGG
GCTGAAGTTAAAGAAGGTGATATCTTAGTAGGGAAAACTTCACCAAAAGGTCAAGTTGAA
CTAACCCCTGAAGAAAAACTGATCCAAGCAATCTTAGGTGATAAGGTAAAACAAGTTCGT
GAATCATCACTAAAAGTACCAAACGGTGGTGATGGGATCGTTGCTGGAATCAAGCGTTTT
AGCATTGCTAATGGTGATGAATTAGAAGATGACGTTTTAGAACTAGTTAAGGTTTATGTA
GTTCAAAAACGAAAGATTCAGATCGGTGATAAGGTTGCTGGTAGACACGGTAACAAGGGG
ATTATTTCTAAGATCGTTGCCCAAGAAGATATGCCTCACCTTGAAGATGGGACCCCACTT
GATATCTTATTAAACCCATTAGGGGTGCCTAGCCGGATGAACATCGGTCAGATCTTTGAA
TTACATTTAGGTTATGCAGCTAGAGAGTTAGCTAAAGCTAAATTAATCGAAGCTTGTTTT
GATAAGAAACTAGCTGATCAATTAGACACAGTATTTGGTTTAGAAAAATCAAAAACCCAA
AGTTTAATTAAAAACCTAGTTGAACATATGAAATCAATTGGGGTAACTAGTCTTGCACAA
ACTAAGAACCGCGTTCGTACGATCGATATTGATATCGTATTAAAACAATTAGGTTTAACT
TATGATGATTTAGCATTTAAGATTGCCACACCAGTATTTGAAGGTGTGAATATGGAAGAT
CTAAAAGCAATCATGTCAGAAGCTGGGATCGACCCTGATAAGACTGAAGGTAAGTTTAAA
CTGATCGATGGTCGAACTGGTGAACCGTTTGAAAAACCAATCTCAGTTGGTATCATGTAC
ATCTTAAAACTTGACCACATGGTTGATGATAAGATTCACGCCAGAGCAGTTGGTCCTTAT
TCTAAGATTACCCAACAACCACTAGGTGGTAAGTCACAAAACGGTGGTCAAAGATTTGGG
GAGATGGAAGTGTGAGCGCTTCAAGCTTATGGTGCTGCGCATAACCTAAGAGAGATCTTA
ACGATTAAATCAGATGACGTTAGGGGAAGAAATATGACCTACAACGCGATCGTTAAAGGG
TTAAGTATTCCAGAACCTGGTGTACCTGAATCATTTAAGTTATTAACTAAAGAGCTTCAA
GGTTTAGGAATGACGCTTAATGTGTTGTATGATGATGTTTCAATCGAAAATATTAATAAT
ATCTCTGTAGTCGACGAAAGTCTAGAACCAAAAATCCACGATGCCGAGTTTGACACATTT
ACTTTGGATGACTACAACGACGATAACTTCTAA

>protein sequence
VKKNSAFHNTQYSPKVVRRDYSKVHNKYNPLNLPGIQVNAYKQFLEKELEEIIGSYFPIK
SPNGKYSVEFHGMKILDPQITKEEASAESKTYDASLYVNLSLVNHQTGTVKKISKKSAKS
KAEGIYFADIPLMTENGAFIVNGIEKFVVAQIVRSPGAYILNKSQVKLSNSRKRNQEGYI
CEVFPSKGTLMLFYIAENKDFVQAVVRDVGGESAKVFSITTLLKAFGLSEIKIKEIFNNN
DYILRSLESEFYNEKQILNEADIAQLIRDIESDRISKVKSLPIDQKWKNLVLDWYKLNQE
KQELLNSSNPNPTKIESLNTHIGVVLRKLICEKAAKHVIQELSISTRSLDNVSQKEEISY
QSILLQHFFQKKRYDLSSAGRHKFVRKLRVSERLYQRTIAQDIKDLDGNVVIKQGTLMLK
EQLDLFKRLSKEKRLDIIKQIDFINPELNTQFTDVNTYEEVQIYVNNDLRNETTQIIGID
GSNESIETLTLADLISIVSYIVNLPFNIGLYDDIDHLGNKRLKLINELLKSKVQTGMMRI
EKYIKDKLQTADGNNKLADQDAENDPDSDLGTKSSNDLTVKSVINPKPFQIVIKDFFNTH
QLTQFLDQQNPLSELTNKRRISAMGPGGISREDPNLDIRDVHYSHYGRICPIETPEGMNI
GLIMSLAFYATIDKNGFLMTPYLKVENGKITDKVEHLTALREDEYIIAEASSYMDVAKDG
TINNEKIIARYRSSQDLYDPKQVDYIDVSPKQVVSVAASLIPFLENDDSSRALMGANMQR
QATPLLKPYSPLVGTGVEYKIAQDSGMLVTAKNPGTVTYVDSSKITVKGEDNKSTDYNLL
KFVKSNKNTCYNQSPIVAVGDKIETNQALADGPSMKNGELALGQNVLVGFTTWSGYNYED
AVIISDRLFKDDVYSSIHIDEYTIECLRTKNGDEEITRDIPNISESAKRYLDEEGVIMVG
AEVKEGDILVGKTSPKGQVELTPEEKLIQAILGDKVKQVRESSLKVPNGGDGIVAGIKRF
SIANGDELEDDVLELVKVYVVQKRKIQIGDKVAGRHGNKGIISKIVAQEDMPHLEDGTPL
DILLNPLGVPSRMNIGQIFELHLGYAARELAKAKLIEACFDKKLADQLDTVFGLEKSKTQ
SLIKNLVEHMKSIGVTSLAQTKNRVRTIDIDIVLKQLGLTYDDLAFKIATPVFEGVNMED
LKAIMSEAGIDPDKTEGKFKLIDGRTGEPFEKPISVGIMYILKLDHMVDDKIHARAVGPY
SKITQQPLGGKSQNGGQRFGEMEVWALQAYGAAHNLREILTIKSDDVRGRNMTYNAIVKG
LSIPEPGVPESFKLLTKELQGLGMTLNVLYDDVSIENINNISVVDESLEPKIHDAEFDTF
TLDDYNDDNF