GCW_01245

Uniprot: A0A0F6CK98
Description: DNA-directed RNA polymerase subunit beta'
EC number: 2.7.7.6
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). {ECO:0000256|HAMAP-Rule:MF_01322, ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00054052}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. {ECO:0000256|HAMAP-Rule:MF_01322, ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00054030}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoC
Gene names (synonym):
Mass: 145,060
Subunit structure [CC]: SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. {ECO:0000256|HAMAP-Rule:MF_01322}.
Gene ontology (GO): DNA binding [GO:0003677]; DNA-directed RNA polymerase activity [GO:0003899]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003899; GO:0006351
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA polymerase beta' chain family. {ECO:0000256|HAMAP-Rule:MF_01322, ECO:0000256|RuleBase:RU004279}.
Protein families: RNA polymerase beta' chain family
Coiled coil:
Domain [FT]: DOMAIN 293 581 RPOLA_N. {ECO:0000259|SMART:SM00663}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99520
UniPathway: K03046
CDD:
Gene3D:
HAMAP:
InterPro: MF_01322
PANTHER: IPR012754;IPR000722;IPR006592;IPR007080;IPR007066;IPR007083;IPR007081
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF04997;PF00623;PF04983;PF05000;PF04998
SMART:
SUPFAM: SM00663
TIGRFAMs:

In one protein complex with GCW_01240 GCW_03530 287326-291187(+)

>nucleotide sequence
ATGGATAAGCTAAAGAAAAAAAATAAAAATAAACGAATTCGTGGTTTGCAAATCTCGATT
GCTTCAGCTGAAGAGATGAGATCTTGATCTCATGGTGAAGTAAAAAAACCTGAAACGATT
AACTATAAATCATTAAAACCTGAAACTGATGGTTTATTTGATGAAGCAATCTTTGGACCT
GTCAAAGACTTTGAATGTGCTTGTGGTAAGTATAAAAAGATCAAACACCGTGGTCGTACT
TGTGAAAAATGTGGTGTTGAGATTACTGAATCGATCGTAAGAAGAGAACGAATGGGTCAT
ATTGATCTTGCTGTTCCAGTAGCTCACATCTGAATGACTAAAGAATTACCTTCACCTTCA
AAGATCTCTTTAGTATTAGATGTTTCTTATAAAGAAGTTGAAGAGGTAGTTTACTTTGTA
AACTACATTATCTTAAACCCAGGGAATTCAAAAAACCCGGTATTCAAGTTTAAAGAAGTT
GTCGATCTATCTGGTAAGGGCTCTAAATCTGCCAGAATTAAGTTACGTAAAGTTTTACGT
GAAATTAAAGATAAACACCAAGCTGATAAACACTCAATCGTTTATAAACGTGCATCAGAT
TATTACAACAAACTAAAAGAATCACACCTACCGTTTTCAATCGATGAAGTTGCTAAATTC
ATTGAAACACACACAGGAATTAGATTAGGGATTGGTGCTGAAGCGATCTTAGAACTATTA
GAAGGTGTTGATCTACAAAAAGAATATGACTTAATTAATAAAGAATTAAATTCATATTCA
AAAGATTTAAAAGCTAATAAAGAAGATCAAAAAGTTAAAAGAGCACTACGTCGTTTAGAA
ACGATTAAGTGATTAAAAGAATCTGGAATCAAAGCATCTAATATGATCTTAAAAGTGATT
CCAGTTACCCCACCTGACACTAGACCGATCATCCAATTAGATGGGGCGCGATTTACTACT
AGTGATATTAATAACTTCTATCGCCGAATCATTATTCGTAATGAACGGTTAAAGAAGATT
ATTGAGCTACGTGCACCTTCTGTGATTCTTAATAATGAAAAAAGAATGCTTCAAGAAGTG
GTTGATGCATTATTTGATAATGCTTCAAGAAAAAAACCGATTACTGCAAAAGATAAACGT
CCACTTAAATCATTAACTGATCGCTTAAAAGGTAAACAAGGTTTATTCAGACAAAACCTA
TTAGGTAAACGGGTAGACTATTCTGGTCGTTCAGTAATTGTGATTGGTCCTGAACTTAAG
ATGTATGAAGTAGGTATTCCTGCTCCTATGATCTTAAAGTTGTTCAAACCATTCATTATC
CGCGAACTAATTATGCGCTTTAATGAAGATGGTCAAGAGATCAAACCGATCGCACCAAAC
ATTAAGATTGCTGAACAGATGATCGCCCAAAAATCTGAACGAATTTGGGATATCGTTGAT
AAGGTAATTAAAGAAAGACCGGTATTACTTAACCGTGCGCCAACATTACACCGATTAGGG
ATCCAGGCTTTTGAACCTAGGATCGTTGATGGGAAAGCAATCCGCTTACACCCATTGGTA
ACAACTGCTTTTAACGCTGACTTTGACGGTGACCAGATGGCTGTTCACGTACCAATTTCT
AAAGAAGCAGTTGCTGAAGCTAGAGCAATTATGTTAGCTTCTTGACATATCTTAGGGCCA
AAAGATGGTAAGCCAGTAGCTACTCCAACCCAAGATATGGTGTTAGGAAACTACTACTTA
ACCACTGAAAAACGGAATGAAAAAGGTGAAGGGTTAATCTTCTCAGACTTTGACCAAGTA
ATCTTAGCTTATGAAGCTAAACAAGTATCAATTCACGCTTTAATTGGGTTGTCAACCAAA
TGTTTAACAAAAAAACCATTTGCTAAACAAGGGATCGTAATTACAACTGTTGGTAAGGCG
ATCATGAACTCAATTATGCCTGAAGAGATGGCTTATCTAAACGATGGTGATAATTTATTA
GAACTTGATGAATCTAATATCGTTTTTGCTGGTGAAGATTTCAAGCAAAAACTAGCTAAA
CGCCCACTTTACAAACCATTCGGCAAGAAAACATTATCTAAGATTATTGAGATTCTTTAT
AAGAATTTCCCACTTCAAAAAGTGCCACAAGTTCTTGATAAGATCAAAGAATTTGGTTTC
AAATACTCAACACTATCATCAACAACAATCTCAGTCTTTGATATCCCAAGATATGATAAC
AAGCAAGAATACATTACTAAAGCTAATGAGATGATTGCTAAACTAAAACACATGTATCAA
AAAGGATTACTAACTGATGATGAACGTTATACGAAAGTAATTCGTTTATGAGCTGATGTT
AAAGATAACGTATCTAAAGATATTAAAGAGATCATTACTCGTCCTGAGTATAAAGAAAAC
TCAATCGTGGTGATTGCTGATTCTGGTGCTAGAGGTAATATCTCAAACTTCACCCAGTTA
TTTGGGATGAGAGGATTGATGTCTAAGTCATATAACTATGACCAAAAGATTAAATCGCAA
GTAATCCGTGATACGATTGAAGTTCCAATTAAACACTCATTCATTGAAGGATTAACGATT
AATGAATACTTCAATTCTTCTTATGGGGCACGTAAGGGGATGACTGATATTGCGATGAAG
ACCTCAAAATCTGGTTATATGACTAGAAAGTTAGTTGATGCAGCGCAAGAAGTAATCATT
AATGATTCTGACTGTAACACGAATAAGGGGATCGTGGTATCAACGATTACCAACTCTTTA
GATGGTGGGATCGTTGAAACTTTAAGTGAAAGAATCGTTACTAGATATACGATCGACCCA
ATTTATGATGAAAAAACTAAGGAACTATTAGTTGATGCTGACACATTAATTACTAGTGAG
TTAGCTGAAAAGATTGCTAAAGCAAACGTAACTAAAGCACTAATTCGTTCACCGATTTAC
TGTCAGTCAACTAAAGGGTTATGTCAGAAGTGTTTTGGTAATGACTTAACTACAAATGAC
CTTGTTCAGATTGGAACTGCGATTGGAGTGATTGCTGCGCAATCAATCGGTGAACCAGGG
ACTCAGTTAACAATGAGAACGTTCCACACTGGTGGTACGGCAAATGAAGGTAATATTACT
CAAGGGTTTGAACGACTAAAACAGATCTTTGATGTAGTCTCACCAAAAGAATGAGAATTA
GCTACGATCGCTGAAAATGAAGGGGTTGTTGAATCGATCACTTCAGATGCAACTGCTAGA
ATCATTCGCATTAAAACTAGATTAGAAGCTGAAGAATACCGTGTTCCGTTTGATGCTGTG
ATCTCAGTTAACCCTAAAGATGTTGTGTACCCAGGTTCTAAACTAACTGAAGGTTCGATC
GACATCAAGCACTTATTACGAGTTGCTGGGATTGAAACAGTAAGACAGTACTTCTTAGAA
GAAGTGCAGAAAGTTTATCGTTTACAAGGGATTGAAATTGCTGATAAGTACGTTGAAGTA
ACGATCAGACAATTAACAAACAAACTTCAAGTAATTGATGTTGGTGATTCTGATTATTTC
GTGGGGCAAACTGTCGATATCAACAAGTTTAGAAAAGAAGTGACGAATATGTTAACCGCC
AACAAACGCCCACCAGTAGCAATTAACCAAGTATTTGGTTTAGATGAAGCTCCAGCTAAG
ACTGGTTCATTCCTATCAGCAGCTTCATTCCAAGACACCAAAAAGATCCTTACTGATGCT
GCTGTAAAAAATCAGATTGACTACTTGGTTGGTCTGAAAGAAAACGTGATCTTAGGTAAC
TTAATTCCAGCTGGTACTGGATTTATGAGTTCTGAAGAGATCATTAAAGCTGGTGAAGAA
GCTTTAGAAAAAGAATATTAA

>protein sequence
MDKLKKKNKNKRIRGLQISIASAEEMRSWSHGEVKKPETINYKSLKPETDGLFDEAIFGP
VKDFECACGKYKKIKHRGRTCEKCGVEITESIVRRERMGHIDLAVPVAHIWMTKELPSPS
KISLVLDVSYKEVEEVVYFVNYIILNPGNSKNPVFKFKEVVDLSGKGSKSARIKLRKVLR
EIKDKHQADKHSIVYKRASDYYNKLKESHLPFSIDEVAKFIETHTGIRLGIGAEAILELL
EGVDLQKEYDLINKELNSYSKDLKANKEDQKVKRALRRLETIKWLKESGIKASNMILKVI
PVTPPDTRPIIQLDGARFTTSDINNFYRRIIIRNERLKKIIELRAPSVILNNEKRMLQEV
VDALFDNASRKKPITAKDKRPLKSLTDRLKGKQGLFRQNLLGKRVDYSGRSVIVIGPELK
MYEVGIPAPMILKLFKPFIIRELIMRFNEDGQEIKPIAPNIKIAEQMIAQKSERIWDIVD
KVIKERPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPIS
KEAVAEARAIMLASWHILGPKDGKPVATPTQDMVLGNYYLTTEKRNEKGEGLIFSDFDQV
ILAYEAKQVSIHALIGLSTKCLTKKPFAKQGIVITTVGKAIMNSIMPEEMAYLNDGDNLL
ELDESNIVFAGEDFKQKLAKRPLYKPFGKKTLSKIIEILYKNFPLQKVPQVLDKIKEFGF
KYSTLSSTTISVFDIPRYDNKQEYITKANEMIAKLKHMYQKGLLTDDERYTKVIRLWADV
KDNVSKDIKEIITRPEYKENSIVVIADSGARGNISNFTQLFGMRGLMSKSYNYDQKIKSQ
VIRDTIEVPIKHSFIEGLTINEYFNSSYGARKGMTDIAMKTSKSGYMTRKLVDAAQEVII
NDSDCNTNKGIVVSTITNSLDGGIVETLSERIVTRYTIDPIYDEKTKELLVDADTLITSE
LAEKIAKANVTKALIRSPIYCQSTKGLCQKCFGNDLTTNDLVQIGTAIGVIAAQSIGEPG
TQLTMRTFHTGGTANEGNITQGFERLKQIFDVVSPKEWELATIAENEGVVESITSDATAR
IIRIKTRLEAEEYRVPFDAVISVNPKDVVYPGSKLTEGSIDIKHLLRVAGIETVRQYFLE
EVQKVYRLQGIEIADKYVEVTIRQLTNKLQVIDVGDSDYFVGQTVDINKFRKEVTNMLTA
NKRPPVAINQVFGLDEAPAKTGSFLSAASFQDTKKILTDAAVKNQIDYLVGLKENVILGN
LIPAGTGFMSSEEIIKAGEEALEKEY