GCW_01370

Uniprot: A0A0F6CKB6
Description: 5-hydroxymethyluracil DNA glycosylase
EC number: 3.2.2.-; 3.2.2.23; 4.2.99.18
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. {ECO:0000256|SAAS:SAAS00020866}.; CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. {ECO:0000256|SAAS:SAAS00557314}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|SAAS:SAAS00610166};
Enzyme regulation:
Function [CC]: FUNCTION: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. {ECO:0000256|SAAS:SAAS00348515}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): mutM
Gene names (synonym):
Mass: 31,991
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|SAAS:SAAS00020849}.
Gene ontology (GO): damaged DNA binding [GO:0003684]; oxidized purine nucleobase lesion DNA N-glycosylase activity [GO:0008534]; zinc ion binding [GO:0008270]; base-excision repair [GO:0006284]; nucleotide-excision repair [GO:0006289]
Gene ontology IDs: GO:0003684; GO:0006284; GO:0006289; GO:0008270; GO:0008534
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the FPG family. {ECO:0000256|SAAS:SAAS00557294}.; SIMILARITY: Contains FPG-type zinc finger. {ECO:0000256|SAAS:SAAS00551694}.
Protein families: FPG family
Coiled coil:
Domain [FT]: DOMAIN 2 115 FPG_CAT. {ECO:0000259|PROSITE:PS51068}.; DOMAIN 240 274 FPG-type. {ECO:0000259|PROSITE:PS51066}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99538
UniPathway: K10563
CDD:
Gene3D:
HAMAP:
InterPro:
PANTHER: IPR015886;IPR015887;IPR020629;IPR012319;IPR010979;IPR000214;IPR010663
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51068;PS01242;PS51066
ProDom: PF01149;PF06831;PF06827
SMART:
SUPFAM: SM00898;SM01232
TIGRFAMs: SSF46946;SSF81624
324831-325656(+)

>nucleotide sequence
ATGCCAGAACTACCAGAAGTCCAAACGGTGATTAATTATCTTAAAACTAAGATTATTAAT
CAAAAGATTAACAATGTCATTGTTAGCGCACTAAAAGTGTTAAAAAACGCGACACCAAAA
GAATTTAAGAAATTCCTTGTCAATGAACACTTTGTTGACATCAAAAGAATAGGTAAGTAT
ATCATCTTTATTTTGTCTAACAACAAAGTGTTAGTTTCCCACCTAAGAATGGAGGGAAAA
TATAAGATTAGTCAATTTAAAGCTAAGTATGATGAACGTCATGTGTTAGTACGATTTATC
TTAGATGATTTTGAACTGCACTATCATGACACAAGAAGATTTGGGACGTTTCATATTCAT
AGTGTTCTTGATTACCAAGATCAAGATTATTTAAAGAAATTAGCAATTGATCCCACACAA
GAAGAATGAGATTGAAAATACCTAAAAAATAATGCTCAAAAATCTTCAAGGGTAATTAAA
TCAGTTTTATTAGATCAATCTGTGGTTGCTGGGATTGGTAATATTTATGCTGATGAGATC
TTATTCTTATCTAAGATTAATCCAGCTAAAAAAGCCAACGAACTAACTGATCAACAGTTT
AAAGAAATTAGTAAGAATGCAACTAAAGTTTTATTAAAAGCGATCGAACTAAACGGGACA
ACGATCTTTTCTTATCAGTTTAAAGAAAACCATGCTGGTTCATATCAGAATTATTTAAAT
GTCCATTTACAAAAAGATAAACCATGTAAGGTTTGTGGTAACTTAGTGAAAAAAACTAAA
TTAAATAACCGGGGAACTTATTATTGTGCCAAATGTCAAAAGTAG

>protein sequence
MPELPEVQTVINYLKTKIINQKINNVIVSALKVLKNATPKEFKKFLVNEHFVDIKRIGKY
IIFILSNNKVLVSHLRMEGKYKISQFKAKYDERHVLVRFILDDFELHYHDTRRFGTFHIH
SVLDYQDQDYLKKLAIDPTQEEWDWKYLKNNAQKSSRVIKSVLLDQSVVAGIGNIYADEI
LFLSKINPAKKANELTDQQFKEISKNATKVLLKAIELNGTTIFSYQFKENHAGSYQNYLN
VHLQKDKPCKVCGNLVKKTKLNNRGTYYCAKCQK