GCW_01640







Uniprot: A0A0F6CKF8
Description: signal recognition particle protein Srp54
EC number: 
Annotation score: 2 out of 5
Miscellaneous [CC]: 
Protein existence: Inferred from homology
Catalytic activity: 
Cofactor: 
Enzyme regulation: 
Function [CC]: FUNCTION: Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. {ECO:0000256|HAMAP-Rule:MF_00306}.
Pathway: 
Active site: 
Binding site: 
Calcium binding: 
DNA binding: 
Metal binding: 
Nucleotide binding: NP_BIND 107 114 GTP. {ECO:0000256|HAMAP-Rule:MF_00306}.; NP_BIND 189 193 GTP. {ECO:0000256|HAMAP-Rule:MF_00306}.; NP_BIND 247 250 GTP. {ECO:0000256|HAMAP-Rule:MF_00306}.
Site: 
Gene names (primary): ffh
Gene names (synonym): 
Mass: 49,867
Subunit structure [CC]: SUBUNIT: Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. {ECO:0000256|HAMAP-Rule:MF_00306}.
Gene ontology (GO): signal recognition particle [GO:0048500]; 7S RNA binding [GO:0008312]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; SRP-dependent cotranslational protein targeting to membrane [GO:0006614]
Gene ontology IDs: GO:0003924; GO:0005525; GO:0006614; GO:0008312; GO:0048500
Chain: 
Signal peptide: 
Domain [CC]: DOMAIN: Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC. {ECO:0000256|HAMAP-Rule:MF_00306}.
Sequence similarities: SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily. {ECO:0000256|HAMAP-Rule:MF_00306}.
Protein families: GTP-binding SRP family, SRP54 subfamily
Coiled coil: 
Domain [FT]: DOMAIN 2 87 SRP54_N. {ECO:0000259|SMART:SM00963}.; DOMAIN 99 243 AAA. {ECO:0000259|SMART:SM00382}.; DOMAIN 100 295 SRP54. {ECO:0000259|SMART:SM00962}.
Motif: 
Region: 
EMBL: CP006916
ProteinModelPortal: 
MEROPS: 
EnsemblBacteria KO: AHB99580
UniPathway: K03106
CDD: 
Gene3D: 
HAMAP: 1.10.260.30;3.40.50.300
InterPro: MF_00306
PANTHER: IPR003593;IPR027417;IPR013822;IPR004125;IPR022941;IPR000897;IPR004780
PIRSF: PTHR11564:SF7
PRINTS: 
PROSITE: 
Pfam: 
ProDom: PF00448;PF02881;PF02978
SMART: 
SUPFAM: SM00382;SM00962;SM00963
TIGRFAMs: SSF47364;SSF47446;SSF52540
383515-384853(-)

>nucleotide sequence
TTAGAATAATGAACCAAAGGGATTCTTGTTAGCTTTTAACATTCGACCGATCTCTTCCAT
CTTGCCAGAAGCATCTTGTCAGTCTTTTAATAACTTATTTAATTCTTCAACTTTTCTTCC
CGAACCCTTAATAATTCGGCTTTTTCTTGAAGAGTTTTTTATTAATAATTTGGGATTTCT
TCGTTCTTTTAATGTCATTGATGACATTAAAATTTTTCACACATGGATTTTTTGAGTGGC
TTCTTCAACTTTATCTTCACTAATCTTATTAGCAAATCCTGGAAGCATTTTTACAAGTGA
ACCTAATCCACCCATTTTAGTTATTGATTGGGTTTGTTGTAATAAATCTTCAAGATCCAT
TCGACCTGAAAGCATCTTTTCGAAAGTTTTCATCGTTGATTTTTCATCATAAACTTCCTG
TGCTTTCTCAGCCAAAGTCATTACGTCACCAAATCCTAAGATGCGATCGGCAATCCGTTC
AGGATAAAATAACTCAATCGCATCAAGTTTTTCACCGCTTGTTGTAAACTTGATCGGCAC
GTTTAAGATCGATCTTAATGATAAAGCAGCACCTGCTCTAGCATCACTATCTAGTTTGGT
AATAATGATCCCGCTTAGTTTTAGGTAATTATTAAATTCTGTGGCGATATTAATGATCTC
TTGACCAGATAGACCATCCACTACTAAGATAATCTCATCAGGATCAACAGTGTTACGGAT
ATTAACTAATTCGTTCATTAATTCGACATTAGTTTGTAATCGTCCAGCAGTATCATAAAT
GATCAGATCACAATTTTTTTCTTTTGCGATCTCAATTGAGTTTTTAGCTGTTTTAACTGG
ATCAGATAATCCTTCATTATAGAATGGCATATTGATCTGTTCAGCTAAAGTATTAAGTTG
ATCAATTGCTGCAGGACGATAAACGTCTAGTCCAACTAATAAGCTGTTCTTTTGTTTTTG
TTTATTTAAGAATTTAGCTAATTTAGCTACAGTAGTAGTTTTCCCAGAACCTTGTAAACC
AACTAACATAATCTTAGCTAATTTCTTATCAATTTTTAGGGGTTGTTGATCTTTACCTAA
GATATTAACAAGTTCATCTTTGATGATCTTTAAGAAATAAGCATGAACATCAGTGTTACG
ATCAACAGCTTGTCCGATCGCTTTTTCTTTAATCGCTTTAATAAAGGTCTTAACAACATT
AATATTTACGTCAGCATCTAATAAAGCGATTCGAATCTCACGTAAGACTTCAGTAAGATC
TTCTTCTTTAATGGTTTGATTTAGTAATCTTTTCTTTAACGATTTAGTAACAATCGATGA
GATCATTGATTTAAGCAT


>protein sequence
MLKSMISSIVTKSLKKRLLNQTIKEEDLTEVLREIRIALLDADVNINVVKTFIKAIKEKA
IGQAVDRNTDVHAYFLKIIKDELVNILGKDQQPLKIDKKLAKIMLVGLQGSGKTTTVAKL
AKFLNKQKQKNSLLVGLDVYRPAAIDQLNTLAEQINMPFYNEGLSDPVKTAKNSIEIAKE
KNCDLIIYDTAGRLQTNVELMNELVNIRNTVDPDEIILVVDGLSGQEIINIATEFNNYLK
LSGIIITKLDSDARAGAALSLRSILNVPIKFTTSGEKLDAIELFYPERIADRILGFGDVM
TLAEKAQEVYDEKSTMKTFEKMLSGRMDLEDLLQQTQSITKMGGLGSLVKMLPGFANKIS
EDKVEEATQKIHVWKILMSSMTLKERRNPKLLIKNSSRKSRIIKGSGRKVEELNKLLKDW
QDASGKMEEIGRMLKANKNPFGSLF






















© Fisunov Lab of Proteomics, 2016.