GCW_01645
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Uniprot: A0A0F6CKF9
Description: tyrosyl-tRNA synthetase EC number: 6.1.1.1 Annotation score: 3 out of 5 Miscellaneous [CC]: Protein existence: Inferred from homology Catalytic activity: CATALYTIC ACTIVITY: ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). {ECO:0000256|HAMAP-Rule:MF_02006, ECO:0000256|SAAS:SAAS00105588}. Cofactor: Enzyme regulation: Function [CC]: FUNCTION: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). {ECO:0000256|HAMAP-Rule:MF_02006, ECO:0000256|SAAS:SAAS00009764}. Pathway: Active site: Binding site: BINDING 33 33 Tyrosine. {ECO:0000256|HAMAP-Rule:MF_02006}.; BINDING 161 161 Tyrosine. {ECO:0000256|HAMAP-Rule:MF_02006}.; BINDING 165 165 Tyrosine. {ECO:0000256|HAMAP-Rule:MF_02006}.; BINDING 228 228 ATP. {ECO:0000256|HAMAP-Rule:MF_02006}. Calcium binding: DNA binding: Metal binding: Nucleotide binding: Site: Gene names (primary): tyrS Gene names (synonym): Mass: 47,935 Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_02006, ECO:0000256|SAAS:SAAS00009771}. Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; RNA binding [GO:0003723]; tyrosine-tRNA ligase activity [GO:0004831]; tyrosyl-tRNA aminoacylation [GO:0006437] Gene ontology IDs: GO:0003723; GO:0004831; GO:0005524; GO:0005737; GO:0006437 Chain: Signal peptide: Domain [CC]: Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_02006, ECO:0000256|SAAS:SAAS00538763}.; SIMILARITY: Contains 1 S4 RNA-binding domain. {ECO:0000256|HAMAP-Rule:MF_02006}. Protein families: Class-I aminoacyl-tRNA synthetase family, TyrS type 1 subfamily Coiled coil: Domain [FT]: DOMAIN 350 414 S4 RNA-binding. {ECO:0000259|PROSITE:PS50889}. Motif: MOTIF 38 47 "HIGH" region. {ECO:0000256|HAMAP-Rule:MF_02006}.; MOTIF 225 229 "KMSKS" region. {ECO:0000256|HAMAP-Rule:MF_02006}. Region: EMBL: CP006916 ProteinModelPortal: MEROPS: EnsemblBacteria KO: AHB99581 UniPathway: K01866 CDD: Gene3D: HAMAP: 3.10.290.10;3.40.50.620 InterPro: MF_02006 PANTHER: IPR001412;IPR002305;IPR014729;IPR002942;IPR002307;IPR024088;IPR024107 PIRSF: PTHR11766 PRINTS: PROSITE: PR01040 Pfam: PS00178;PS50889 ProDom: PF00579 SMART: SUPFAM: TIGRFAMs: 384858-386106(-) >nucleotide sequence TTGATCTTTTCTAACATTTAGTTGTGCTTGTGGATCAGTTATTTTCAGATCATCAATCTG AATTGATCCTTGAGCTATTAGTTCACGTAAGATTCTTTTTGAATCAGCAACCTTTGCTTG CAACAAGAAATCAATAACCATAAACGATTGATTTCTATCTAGTTCAACTTTATCAAAACT AGCAAAAACAACTAATTTTTCTTGATCAGACAATTGATCTAGTTGATTATCAAATAATGC CTTGTTCATCTTGTGAACAGTATCTAAATGTTCTTGAGAATGAATAAATTTAACTAGATA ATCTGCTAAGAATTTTTGCGCAATTCTTGTCTTTTTATTAGCTAGATGTGCCTCAATTAG TTCTTGTAATTGTTCAAGTGATTGATCTAAAACATAACGATGGATTAGATCAATAATAAA CTGATCGTCTTGGTTATAGATATATTGTCAAATTAGGTATGGATGAGTTAAGTTTTCATC TAAATAAAGTGCACCTTGTTCTGATTTACCGAACTTATGACCATTGAGATTAAGTAATAA ATTAAAGGTTAACCCACATAAATAAGGAGTTTTTTCTTCACCATATTTTCTTTTGATCAG ATCAATTCCGGTTGTTATATTTCCTCATTGATCAGATCCGCCACACTGAACTTGGACATC GTATTGTTCATACAGATGTAAAAAGTCATGACCCTGAATTATGTTGTATGAAAACTCAGC ATAAGAGATCCCATTTTCAATTCTTGAACGAATGAATTCTTTATCTAGTAAATAACCAAT ATTGATTAATTTTCCCGTATCTCTTAAAAAATTTAAGAGATTCATATTTTGATAAAAATC AATGTTGTTAATGATCTTGGCTTGAGTATAACGTTCTAGTTGTACTTTGATCTTATTAGC GTTAGCTGTGATTTTTTCATAATCTTGAACCTTACGTTCAGAGGTCTTACCTGAAGGGTC ACCGATCAAACCAGTAGCCCCCCCTAAGATAGCATAAGTTTTAAACCCAACTTTTTTTAA GTATCTTAAAACGATGATTGGAATTAAATTCCCTAAATGTAAAGATTCACCACTAGGGTC GAAACCAACATAAACACCCTTTTGATTTTTTAGGGCTAGTGCTAATTTTTCTTCATTAGA AATCTGTTTGATAATATTGCGTTTTTTAAGTTCAGAAATAAAGTCCAT >protein sequence GFKTYAILGGATGLIGDPSGKTSERKVQDYEKITANANKIKVQLERYTQAKIINNIDFYQ NMNLLNFLRDTGKLINIGYLLDKEFIRSRIENGISYAEFSYNIIQGHDFLHLYEQYDVQV QCGGSDQWGNITTGIDLIKRKYGEEKTPYLCGLTFNLLLNLNGHKFGKSEQGALYLDENL THPYLIWQYIYNQDDQFIIDLIHRYVLDQSLEQLQELIEAHLANKKTRIAQKFLADYLVK FIHSQEHLDTVHKMNKALFDNQLDQLSDQEKLVVFASFDKVELDRNQSFMVIDFLLQAKV ADSKRILRELIAQGSIQIDDLKITDPQAQLNVRKDQQLTVIKKGKKNYFIVVWKG |
© Fisunov Lab of Proteomics, 2016.