GCW_01785

Uniprot: A0A0F6CKI0
Description: phosphoglycerate kinase
EC number: 2.7.2.3
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|RuleBase:RU000532, ECO:0000256|SAAS:SAAS00318880}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|SAAS:SAAS00318884}.
Active site:
Binding site: BINDING 39 39 Substrate. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|PIRSR:PIRSR000724-1}.; BINDING 123 123 Substrate. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|PIRSR:PIRSR000724-1}.; BINDING 165 165 Substrate. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|PIRSR:PIRSR000724-1}.; BINDING 216 216 ATP. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|PIRSR:PIRSR000724-2}.; BINDING 343 343 ATP. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|PIRSR:PIRSR000724-2}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 369 372 ATP. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|PIRSR:PIRSR000724-2}.
Site:
Gene names (primary): pgk
Gene names (synonym):
Mass: 45,537
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|SAAS:SAAS00318869}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; phosphoglycerate kinase activity [GO:0004618]; glycolytic process [GO:0006096]
Gene ontology IDs: GO:0004618; GO:0005524; GO:0005737; GO:0006096
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the phosphoglycerate kinase family. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|RuleBase:RU000532, ECO:0000256|SAAS:SAAS00551479}.
Protein families: Phosphoglycerate kinase family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 24 26 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|PIRSR:PIRSR000724-1}.; REGION 62 65 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00145, ECO:0000256|PIRSR:PIRSR000724-1}.
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99602
UniPathway: K00927
CDD:
Gene3D:
HAMAP: 3.40.50.1260;3.40.50.1270
InterPro: MF_00145
PANTHER: IPR001576;IPR015901;IPR015911;IPR015824
PIRSF: PTHR11406
PRINTS: PIRSF000724
PROSITE: PR00477
Pfam: PS00111
ProDom: PF00162
SMART:
SUPFAM:
TIGRFAMs: SSF53748
409664-410906(+)

>nucleotide sequence
ATGATTAACTACAATAAGAAGACCTTAAAGGATGTTGATCTAAAAGATAAAACAGTAATT
GTACGTGTTGATTTTAACGTACCAATTAAAGACAATAAGGTTATAGATGATACAAGAATT
GTTCAAGCATTAGATACGATTAAATATCTAATCGAACAAAACTGTAAGATCGTTTTATTA
AGTCACTTAAGCCGAATTAAGTCATTAGAAGATATTAGTTCTAAAAAGAAGAGCTTAAGA
CCAGTTTATGAAAACTTAAAAACCAAACTTAACAACGTTAAGTTTTTAGAAGAAAACGTT
GGTTATGACGTTGTTGAAGCGGTTAAACAATTAAAACACCAAGAAGTTTTATTATTGGAA
AACACTCGTTATAACGATGTCGACAATCAAGGTGAAGTTGTTAAAAAAGAATCTAAAAAC
AGTCCAGAACTAGGACGTTTCTGAGCTAGTTTAGCTGATGTTTTTGTCAACGACGCATTT
GGTACTTCACACCGTGCGCATGCTTCAAACGTTGGGATTGCTGCAAATATCTCGCAATCT
TGTATTGGTTTCTTAGTTCAAAAAGAACTAGAAGCATTATCAAAATTAACAAACAACCCA
CAACGTCCATTTGTTGTGATCTTAGGGGGTGCTAAGGTATCAGATAAATTAAAAGTAATC
GAATCATTACTAAAATCTGCTGATCATATTCTAATCGGTGGGGGAATGGTTAATACCTTT
AATATGGCTAAAGGTTATCATATTGGTAAATCATTATTCGAACCTGAGATGTTAGAAACT
GCTAAGAAGATCTTAGCTGAAGATAAGGATAATAAGATCATTTTAGCGACTGATCAGATG
GTAACTAAAGCTAGTACGATCACTGATATTAAAACTGCACCTGCTGGTAAGTGTGTGTTT
GCCAAAGATGAAGCAGAAAACGAAGATTTTGAAGCTTTAGATATCGGTGATGAATCAATC
AAAACTTTCAAATCTTATATAGCTAAAGCTAAATCAATCTTTTGAAACGGTCCTTTAGGT
GTTTTTGAAAACCCTAATTACGAACGTGGATCTTATGAGATTGCTAAAGCGATCAGTGAA
TCTGATAGTTATAGTGTGATCGGTGGCGGTGATAGTGCTGCTGCTGCAAACCAATTTAAA
TTAGCCGACAAATTTAGCTTTGTCTCAACTGGTGGTGGTGCTTCATTAACATTTATGGAA
CAAACTGTTCTACCAGGGATTGAAGCGATCCAATCAAAATAA

>protein sequence
MINYNKKTLKDVDLKDKTVIVRVDFNVPIKDNKVIDDTRIVQALDTIKYLIEQNCKIVLL
SHLSRIKSLEDISSKKKSLRPVYENLKTKLNNVKFLEENVGYDVVEAVKQLKHQEVLLLE
NTRYNDVDNQGEVVKKESKNSPELGRFWASLADVFVNDAFGTSHRAHASNVGIAANISQS
CIGFLVQKELEALSKLTNNPQRPFVVILGGAKVSDKLKVIESLLKSADHILIGGGMVNTF
NMAKGYHIGKSLFEPEMLETAKKILAEDKDNKIILATDQMVTKASTITDIKTAPAGKCVF
AKDEAENEDFEALDIGDESIKTFKSYIAKAKSIFWNGPLGVFENPNYERGSYEIAKAISE
SDSYSVIGGGDSAAAANQFKLADKFSFVSTGGGASLTFMEQTVLPGIEAIQSK