GCW_01820

Uniprot: A0A0F6CKI6
Description: rRNA methyltransferase
EC number: 2.1.1.207
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu). {ECO:0000256|HAMAP-Rule:MF_01885}.; CATALYTIC ACTIVITY: S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA. {ECO:0000256|HAMAP-Rule:MF_01885}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Could methylate the ribose at the nucleotide 34 wobble position in tRNA. {ECO:0000256|HAMAP-Rule:MF_01885}.
Pathway:
Active site:
Binding site: BINDING 110 110 S-adenosyl-L-methionine; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_01885, ECO:0000256|PIRSR:PIRSR029256-1}.; BINDING 131 131 S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_01885, ECO:0000256|PIRSR:PIRSR029256-1}.; BINDING 139 139 S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_01885, ECO:0000256|PIRSR:PIRSR029256-1}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): cspR
Gene names (synonym):
Mass: 19,142
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; RNA binding [GO:0003723]; S-adenosylmethionine-dependent methyltransferase activity [GO:0008757]; tRNA methyltransferase activity [GO:0008175]
Gene ontology IDs: GO:0003723; GO:0005737; GO:0008175; GO:0008757
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily. {ECO:0000256|HAMAP-Rule:MF_01885}.
Protein families: Class IV-like SAM-binding methyltransferase superfamily, RNA methyltransferase TrmH family, TrmL subfamily
Coiled coil:
Domain [FT]: DOMAIN 5 151 SpoU_methylase. {ECO:0000259|Pfam:PF00588}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99608
UniPathway: K03216
CDD:
Gene3D:
HAMAP: 3.40.1280.10
InterPro: MF_01885
PANTHER: IPR029028;IPR001537;IPR016914;IPR029026
PIRSF:
PRINTS: PIRSF029256
PROSITE:
Pfam:
ProDom: PF00588
SMART:
SUPFAM:
TIGRFAMs: SSF75217
417940-418432(-)

>nucleotide sequence
TTATTTAACGTTTTTAAATTGATTTTGCCGACTTAATTCATATAAAGCACAGATCACGCT
GTTAGCTAGATTAAAACTTCTGATCGCATTGTGCATGGGAATTTTTACTAAATTGTTCTG
ATATTTGTTTAATAATTGCTCAGGTAAGCCATTAGTTTCTTGACCAAAAACTAAATAAAT
TCTTTGATCATGATCCAAATATTCTTTAAAATCAATATTTGATAAATAGTTATTAGCTTT
TTTAGTAAATAAAAATAAATTGTCATCAATCTGATTTTGTTTAATAAAGTCGTCAAAATC
AGCATATTGTTTTAAATTAAGATGATCCCAATAATCTAAACCAGCTCGCTTAAATCGTTC
ATCTGTAATAAAAAAACCAAATGGATGGATCAGATGTAAAGTCGCATTAAAACCAACACA
ACTTCTTGCGATGTTGCCAGCGTTTCTTGGGTCTTGTGGTTGGTATAAAACAACATTAAT
TTTTGATGACAT

>protein sequence
MSSKINVVLYQPQDPRNAGNIARSCVGFNATLHLIHPFGFFITDERFKRAGLDYWDHLNL
KQYADFDDFIKQNQIDDNLFLFTKKANNYLSNIDFKEYLDHDQRIYLVFGQETNGLPEQL
LNKYQNNLVKIPMHNAIRSFNLANSVICALYELSRQNQFKNVK