GCW_02000

Uniprot: A0A0F6CKL4
Description: 1-deoxy-D-xylulose-5-phosphate synthase
EC number: 2.2.1.7
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO(2). {ECO:0000256|SAAS:SAAS00085919}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|SAAS:SAAS00611643}; ; COFACTOR: Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000256|SAAS:SAAS00611644};
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP). {ECO:0000256|SAAS:SAAS00570402}.
Pathway: PATHWAY: Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. {ECO:0000256|SAAS:SAAS00085918}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary):
Gene names (synonym):
Mass: 66,803
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|SAAS:SAAS00085924}.
Gene ontology (GO): 1-deoxy-D-xylulose-5-phosphate synthase activity [GO:0008661]; metal ion binding [GO:0046872]; 1-deoxy-D-xylulose 5-phosphate biosynthetic process [GO:0052865]; terpenoid biosynthetic process [GO:0016114]; thiamine biosynthetic process [GO:0009228]
Gene ontology IDs: GO:0008661; GO:0009228; GO:0016114; GO:0046872; GO:0052865
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the transketolase family. DXPS subfamily. {ECO:0000256|SAAS:SAAS00570410}.
Protein families: Transketolase family, DXPS subfamily
Coiled coil:
Domain [FT]: DOMAIN 266 432 Transket_pyr. {ECO:0000259|SMART:SM00861}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99636
UniPathway: K01662
CDD:
Gene3D:
HAMAP: 3.40.50.970
InterPro:
PANTHER: IPR005477;IPR029061;IPR005475;IPR033247
PIRSF: PTHR11624
PRINTS:
PROSITE:
Pfam:
ProDom: PF13292;PF02779
SMART:
SUPFAM: SM00861
TIGRFAMs: SSF52518
473174-474920(-)

>nucleotide sequence
TTACTTTTTTAAGTTCTTTTTAATTGCTTCAATCACTTGTTCATGACCCATCTCGTGTTT
GATTAATAGATCTTTATTATCTCCGTGTTGATGGATCGTTTTGAAATTATAAGGATATAA
TGATTTATTTTGGTATTTATTTTCAACGATAAGTCGATAAAATTCATTAGCCAACCCAAG
ATTTTGATAAATCTCTTCATAAAGATAGATCTGATCATATTGATCTAAGACCTTAGTGAT
CATTTGATCTAATTGATCACCATGAACTAAGATTGCATCAAAGCAATCAACATCATTAAG
TTGTTGTTGATCTAACAACCTCTTAAATAAGATCGGGTTGTTATTATATGAAATTAATGC
TATTTTATTTTTAGTATTAGCTGGATTTAACTTAACCCATTGATCAATATGATCAAAGTC
TTGATAATACGATTCGTATTGGTTAAGTTCAACAAAATAGCGATTATACCTAATTGCGAT
TACTTGAGTTGTATTATTAATTACTTGATCAAGTAACCTTTTTAATACGATCTGATTACT
AGCAGCATAGATCTTAGCATGTGGGGTGTTTTTAATCATCCCCACATCAAAGATCCCATG
GTGAGATGAACCTTCAGTTCCATTCAATCCACATCGATCAATCAAGAGATTGATCCCTAA
ATTTAATCGTGATAAATCATGGATTAATTGATCATAACTTCTTTGTAAAAAGGTTGAATA
GATCGGTAAAAAGACCGGAACCTGATTTAAACTTACTCCAGAAGCCATGGATAATGCATG
TTCTTCAGAGATTCCAACATCAATGTAGTTCTTTGGATATTTCTTAATCAGATCAATAAA
TCCAGTTGCATAAGTCATGGCGGGATTAATCATGCAAAACTGTTTTTTTTCTTTTAGTTT
TGCTTCTAAATAACTTGCAGCAATAAACCCAGATTGGTCTTGGTAAGTTTTATTAGATCG
ATTGGAATTATGATAAGTTCCAATCAGATCTGCTTCAGCTTGTTTAATCCCCTTACCCTT
GATCGTTTTAACATGAACGATCACTGGTTTTTGTTTAACCAACTCTTTAGCTTTTTCTAA
CCCAGCAAAGATTTGATCTAAGTCGTGACCATCTTCAATCTTGAGATACTCATAATTTAA
TTGCCTAAAGAAGTTGGTTTGATCATCTTTTAAGTTGGTTAACAACTTATTAAACTCACC
AAAGTTTTCTGAGATCGACATCTGATTATCATTAAGGATAATAATCATCTTATGATGATG
AAAACTAATATTGTTGATTGCTTCAAAAGCGATCCCATTAGCAATACTACCATCCCCGAT
TACGGGGATGATTAGTTGTTCTTTATCCAGTAGTTGGTTACTAAAAACTAACCCACTAGC
TACACTAAGTGAATTTGAAGTATGACCACCAGCATAATAGTCATACTTAGATTCGTTTAG
ATCTTGTAATCCACTTAACCCATTTGTTTCACGGATCGATCTCATCCGATCAAACCGACC
AGTCACCATCTTATGAACATAAGCTTGGTGACTGGTATCATAAAAGACTTTATCTCGATC
TAGATTAAAAAAGTATGCTAGTGCAATACTTAGTTCAACAATCCCTAGATTGGATGATCA
GTGGATACTTTTTTCTTTACCAAGATTAATCAGATAATCCCTGATCTCAGTTGCTAATTG
TTTAAGAGCAGCTTTTTTGATCTTCAAAAAATCAGCATAACTTTTAATCGTATTTAATAG
TTTCAT

>protein sequence
MKLLNTIKSYADFLKIKKAALKQLATEIRDYLINLGKEKSIHWSSNLGIVELSIALAYFF
NLDRDKVFYDTSHQAYVHKMVTGRFDRMRSIRETNGLSGLQDLNESKYDYYAGGHTSNSL
SVASGLVFSNQLLDKEQLIIPVIGDGSIANGIAFEAINNISFHHHKMIIILNDNQMSISE
NFGEFNKLLTNLKDDQTNFFRQLNYEYLKIEDGHDLDQIFAGLEKAKELVKQKPVIVHVK
TIKGKGIKQAEADLIGTYHNSNRSNKTYQDQSGFIAASYLEAKLKEKKQFCMINPAMTYA
TGFIDLIKKYPKNYIDVGISEEHALSMASGVSLNQVPVFLPIYSTFLQRSYDQLIHDLSR
LNLGINLLIDRCGLNGTEGSSHHGIFDVGMIKNTPHAKIYAASNQIVLKRLLDQVINNTT
QVIAIRYNRYFVELNQYESYYQDFDHIDQWVKLNPANTKNKIALISYNNNPILFKRLLDQ
QQLNDVDCFDAILVHGDQLDQMITKVLDQYDQIYLYEEIYQNLGLANEFYRLIVENKYQN
KSLYPYNFKTIHQHGDNKDLLIKHEMGHEQVIEAIKKNLKK