GCW_02190

Uniprot: A0A0F6CKP3
Description: RNA polymerase sigma factor
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. {ECO:0000256|HAMAP-Rule:MF_00963, ECO:0000256|SAAS:SAAS00535554}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding: DNA_BIND 602 621 H-T-H motif. {ECO:0000256|HAMAP-Rule:MF_00963}.
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoD
Gene names (synonym): sigA
Mass: 73,898
Subunit structure [CC]: SUBUNIT: Interacts transiently with the RNA polymerase catalytic core. {ECO:0000256|HAMAP-Rule:MF_00963}.
Gene ontology (GO): cytoplasm [GO:0005737]; DNA binding [GO:0003677]; sigma factor activity [GO:0016987]; transcription factor activity, sequence-specific DNA binding [GO:0003700]; transcription initiation from bacterial-type RNA polymerase promoter [GO:0001123]
Gene ontology IDs: GO:0001123; GO:0003677; GO:0003700; GO:0005737; GO:0016987
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily. {ECO:0000256|HAMAP-Rule:MF_00963}.
Protein families: Sigma-70 factor family, RpoD/SigA subfamily
Coiled coil: COILED 306 329 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 425 438 RNA_pol_sigma70. {ECO:0000259|PROSITE:PS00715}.; DOMAIN 601 627 RNA_pol_sigma70. {ECO:0000259|PROSITE:PS00716}.
Motif: MOTIF 425 428 Interaction with polymerase core subunit RpoC. {ECO:0000256|HAMAP-Rule:MF_00963}.
Region: REGION 401 471 Sigma-70 factor domain-2. {ECO:0000256|HAMAP-Rule:MF_00963}.; REGION 480 561 Sigma-70 factor domain-3. {ECO:0000256|HAMAP-Rule:MF_00963}.; REGION 574 629 Sigma-70 factor domain-4. {ECO:0000256|HAMAP-Rule:MF_00963}.
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99665
UniPathway: K03086
CDD:
Gene3D:
HAMAP: 1.10.10.10
InterPro: MF_00963
PANTHER: IPR014284;IPR000943;IPR007627;IPR007624;IPR007630;IPR013325;IPR013324;IPR012760;IPR028630;IPR011991
PIRSF:
PRINTS:
PROSITE: PR00046
Pfam: PS00715;PS00716
ProDom: PF04542;PF04539;PF04545
SMART:
SUPFAM:
TIGRFAMs: SSF88659;SSF88946

In one protein complex with GCW_01240 GCW_01245 GCW_03530 514073-516014(-)

>nucleotide sequence
TTAATTTTCTGAGTCATGTTTTAAGAAAGAACGTAATCTCGCATTTTTAGAAGGGTGTTT
TAATTTTCTTAAAGCTTTAGATTCAATCTGACGAATTCTTTCTCTTGTTACATTGAACTT
TTGACCAACTTCTTCAAGGGTCATTTGTTGGTGTCAAGGGTGTAACCCATAGCGCATTCT
GATAATCTGTTCTTCTTTTTTTGTTAATGTGTTAACAAAAAGCTCATTGATATGTTCAGT
AACAATTAGTTTTTCAGTGTATTCGTCTGGTTTTAAGATATCACGATCTTTAACAAAATC
AACGAATTGTGATTCTTCATCGTGACCAATCGGTTTATCTAATGAAACTGGTTCTTGGTT
TAACTTTTTAATATCAGCAACTTTTTTAGGGGTAAATCCGTTAACTTGACCACCCATTGC
TTCAGTTAATTCTTCTAATGTTGGATCTCTTCCAAGTTGTTGAGTCAACATTCTTTCAGT
TTTAATTAACTTATTGATCGTTTCAACCATATGAACAGGAATTCTTACTGTTCTTGCTTG
ATCAGCAATCGCTCTTGTAATCGCTTGTCTGATCCATCATGTTGCATAAGTTGAGAACTT
GTTTCCCAGTTTATAATTAAACTTAGAGATTGCTTTCATCAATCCGATCGAACCTTCTTG
AATAAGATCAACGAAATCTAAACCTCTGTTTAGATATTTTTTTGCAATTGAAGTAACTAA
TCTTAAGTTAGATGTTACTAATTGGTTGATCGCATATTCTCTAGTTTCAGGATCAGTACT
TCCTAGTAGTTTAGCAATCTGAATTTCTTCACCAAAATTAAGGATCTTAGATGAACCTAA
TGTTCCTAAAAACGCTTTAACATTATCTTCAACTTTATCTTTAGTTGAAGCACCTTTACT
AATAAGATCATCTAAGTTCTTATCGATTTCACTTAGATCTTGGTTTTTTCTAAATAATGA
GATCTTTTCTTCAACATTATCTTCTAATTTAATCTTATTGTCTTTTAATTCTTCAAGAAT
TTCTAAATCTTCAGCTTCAGTGAACTCGTAGTTTTTGAAAACATCAATAATCTCTTCATT
TGATAAAGATTTAGTTGCTTTAGCCTTAGCTAAAAGGGTTTTAATAGCTCGAATTGATTC
TTGATCGTTATTCTTTGTTTTAGATGAAGCTTCTAATACTTCGTGATAAACTAAATCGTT
TTTCTCATTGCTAGGAGCATGCTTAGGTTTACGACCTCTTTGGCGCTTTTGCGCTGTAAT
CGGTTGGTCAATATCTAGTTTACTATCACTGAAAAAATCAGATAAATTAAATTCTTTATC
GTCTAATTCATCTTGCTCACTGAAATCAGTTTCTTTAAAAGCTGATGAATAATTTTCATG
TTTGAAATCACTAAAATCGATCTCTTCGTCTTTACTATCAAAATCAGCAAAATCTATTTC
ATTATTTAAATCAAGATTTTCATCTTTCTTACTAGTTACTTTTTTCTTAGTTGATTTCTT
TGCTGATTTTAATGAAAGCTTACTTTTCTTTTCCTCTTTAGCTACTGATTTAGGTTCAGC
TTTTGCTTTCTTATCTTTAAGGACAACTTCTTTTTTAATAGGTGCAGCTTTAACTTTTTT
AACTGTGGTTTTAGCTTCAGGTTGTTCAACCACTACTTTTTCTGATTTAGCTTTTTTAGC
TTTAGTTGCTACACTTAGTTTTTTCTCACTAACAGGTTTTTCTACTTTAGTAGGCTTTGC
AGTTTTCTTAGTCTTACTTACTGTTTCTATTTTTTTATTCACTAACTTATTAGCTGGTGT
TTTTTCTTGTTTTTTAGCTAATTCAAGTCTTTGTTTTAAAGACAATTGACTAATATTTTT
AGCTTTAGAAACAGTTTTGTTCTTGGGTGTTGAATTTGTATTTTTTGCCATTCTTGTAAA
TGTTAAAGGTATAAAAAGCAA

>protein sequence
LLFIPLTFTRMAKNTNSTPKNKTVSKAKNISQLSLKQRLELAKKQEKTPANKLVNKKIET
VSKTKKTAKPTKVEKPVSEKKLSVATKAKKAKSEKVVVEQPEAKTTVKKVKAAPIKKEVV
LKDKKAKAEPKSVAKEEKKSKLSLKSAKKSTKKKVTSKKDENLDLNNEIDFADFDSKDEE
IDFSDFKHENYSSAFKETDFSEQDELDDKEFNLSDFFSDSKLDIDQPITAQKRQRGRKPK
HAPSNEKNDLVYHEVLEASSKTKNNDQESIRAIKTLLAKAKATKSLSNEEIIDVFKNYEF
TEAEDLEILEELKDNKIKLEDNVEEKISLFRKNQDLSEIDKNLDDLISKGASTKDKVEDN
VKAFLGTLGSSKILNFGEEIQIAKLLGSTDPETREYAINQLVTSNLRLVTSIAKKYLNRG
LDFVDLIQEGSIGLMKAISKFNYKLGNKFSTYATWWIRQAITRAIADQARTVRIPVHMVE
TINKLIKTERMLTQQLGRDPTLEELTEAMGGQVNGFTPKKVADIKKLNQEPVSLDKPIGH
DEESQFVDFVKDRDILKPDEYTEKLIVTEHINELFVNTLTKKEEQIIRMRYGLHPWHQQM
TLEEVGQKFNVTRERIRQIESKALRKLKHPSKNARLRSFLKHDSEN