GCW_02195

Uniprot: A0A0F6CKP4
Description: DNA primase
EC number: 2.7.7.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00079980}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 279 279 Magnesium 1; catalytic. {ECO:0000256|HAMAP-Rule:MF_00974}.; METAL 326 326 Magnesium 1; catalytic. {ECO:0000256|HAMAP-Rule:MF_00974}.; METAL 326 326 Magnesium 2. {ECO:0000256|HAMAP-Rule:MF_00974}.; METAL 328 328 Magnesium 2. {ECO:0000256|HAMAP-Rule:MF_00974}.
Nucleotide binding:
Site:
Gene names (primary): dnaG
Gene names (synonym):
Mass: 77,694
Subunit structure [CC]: SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-Rule:MF_00974}.
Gene ontology (GO): primosome complex [GO:1990077]; DNA binding [GO:0003677]; DNA primase activity [GO:0003896]; magnesium ion binding [GO:0000287]; zinc ion binding [GO:0008270]
Gene ontology IDs: GO:0000287; GO:0003677; GO:0003896; GO:0008270; GO:1990077
Chain:
Signal peptide:
Domain [CC]: DOMAIN: Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
Sequence similarities: SIMILARITY: Belongs to the DnaG primase family. {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00534519}.; SIMILARITY: Contains 1 Toprim domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
Protein families: DnaG primase family
Coiled coil:
Domain [FT]: DOMAIN 273 358 Toprim. {ECO:0000259|PROSITE:PS50880}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99666
UniPathway: K02316
CDD:
Gene3D:
HAMAP: 3.90.580.10;3.90.980.10
InterPro: MF_00974
PANTHER: IPR013264;IPR006295;IPR030846;IPR006171;IPR002694
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50880
ProDom: PF13662;PF08275;PF01807
SMART:
SUPFAM: SM00493;SM00400
TIGRFAMs:

In one protein complex with GCW_03445 516055-518044(-)

>nucleotide sequence
TTATTTTCTTTTTAATAACTCTTCTAAGATGTTGAGATTTTTAATCTGAGCTAACTTAAA
GATCTCACCTTTTGCTAACCATTGAACGAGTTGTTCTTTAGTTTTTAAATCATATAGTTC
TTTAAAACCATCAATCCCACTCGTACACGACTTTAAATAAGCATTTAACTCTTCTTCATT
AACGAATTGATCATGCGCTAATGCAGCATACTTTGAATACTTAATATGAGATAACAACTC
ATTTTTCAACTTATGAAATAGATCCAAATTTAACCTGTTTTCAATATGTTTTTTCATCAA
ATAAACAGTATCATCAAACAATTTGAGCTTGATTTTATTGTAAATCCGATCATTTAAAGT
ATTTTGCTTAGCATTTAATAAATAAAGATCATTCACTTTTCTAAATATTAGATCAGTAAA
ACCTTCAAGAAATCTTTTAGTAAAGTTGTAACAGAATAAAATCGCTTCTAACTGTTCATT
GGTGGTTAAACTTTTCATCACTTGATTGGTTAGAGTGATCTGTAACATCGTATTAAATAA
GTATCTTGTTGGTTTGTAAAGATTAATCTTACTTAAGATCTTCAATCTCACGTTTGATAG
ATCTTTGTAGGTGAGATATTTAAAACTAATTAAATAATAACGACAAATCTTAATTACTGC
TTGGATAAAATTTCTTAACTGTTCAGTGATTCTTTTATCCAGATTGTAATCAATCAGTGT
CGTGATATCAACCTGATCATCCTGTTCAGGTTTAAGTTCATTTAGTTCTTGATACAGTTG
GGTTGATAATTGTTTCTCATCATCATACTCAACGTTTTCTTGTTCTTTTTGGGTTGATAA
TTGCTTTTTAATCAACTCTTTAAGTTGATTTTCACCATCAGGTGAATGATAGATCTCATC
AAGATCTTTTTGTTTAAGATCACTATAATCTAGTTGATAAAGATTATGAACTCTTTTTCT
GGTTAATAACTTAATACATGTGATTGTGGCTTTTTTACCTGATTCATCATTATCTAAAGC
TAATACGATCCTTTTAATCTGATTAAGTTTAGCTTTAATCAATTCAATCTGTTGATCAGT
AAGTGCTGTACCCATGATTGCTACAGCATTCTTAATGCCGATCTGATATAAAGCAATAAC
ATCCATATATCCTTCACACAGATAAATGGTGGAATTATTCTCATCAAGTTCTAATGAAGA
ATAATTATATAAATTATGACCTTTGATGAAATAATCAGTTTCAGGAGTGTTTAGGTATTT
AATTTCGTTATTTTCATCAATAGCTCTACCAGAAAAACCAACAACTTGCCCATCAGAATT
TTTAATCGGAAAGATTACTCGGTTACTAAAATAACAAACGTTTTTTTCTGAAATATATTT
GATGTATCTAAGATTACTTAAGATGATCTGATCATCATAAGTTTTATTTAAATGTTTTTG
ATAAAAAACCTTTAAATCTTCCACTAACTTATTAAGTGAATAACTCTTGGGATGAAAGCC
AATCTGGAAATTATTTAATAGTTCTTCATTGATCAACCCTCTTTTATTTAAGAACTCATT
TAATTTGGGGTTGGTACTAAATTCTAGACGTAAGTTGCTATGAGCTCTTCAAGCTAAAAA
CTGATTTAACTTATAAAATTCTTGATCTGATTCGTCAACAGCTTTTTGCTTATTAAAGTT
TAATGAATAACCGATTGCAGCTAAATCGATCCCTTCAAGTTCTACGATCTTTTTAACTGC
TTCAACAAAATTAAGGTTTTCTTTTTTTTGAACGAATTCAATCACTCCACCACTTTCATT
ACAACTAAAACACTTTCAAACCTGTTTAGTGTCATTAACACTAAAAGAGGGGGTGTTGTC
ATCGTGAAACGGACAAAGTGATTTATAATTTGATCCCTTTTTTTCCAGATCAAGATATTT
TGAAATGATCGAAGATACACTAATCTTCTTACGTAAATATTTGGCTAATTGATTTAAATC
ATTACTCAT

>protein sequence
MSNDLNQLAKYLRKKISVSSIISKYLDLEKKGSNYKSLCPFHDDNTPSFSVNDTKQVWKC
FSCNESGGVIEFVQKKENLNFVEAVKKIVELEGIDLAAIGYSLNFNKQKAVDESDQEFYK
LNQFLAWRAHSNLRLEFSTNPKLNEFLNKRGLINEELLNNFQIGFHPKSYSLNKLVEDLK
VFYQKHLNKTYDDQIILSNLRYIKYISEKNVCYFSNRVIFPIKNSDGQVVGFSGRAIDEN
NEIKYLNTPETDYFIKGHNLYNYSSLELDENNSTIYLCEGYMDVIALYQIGIKNAVAIMG
TALTDQQIELIKAKLNQIKRIVLALDNDESGKKATITCIKLLTRKRVHNLYQLDYSDLKQ
KDLDEIYHSPDGENQLKELIKKQLSTQKEQENVEYDDEKQLSTQLYQELNELKPEQDDQV
DITTLIDYNLDKRITEQLRNFIQAVIKICRYYLISFKYLTYKDLSNVRLKILSKINLYKP
TRYLFNTMLQITLTNQVMKSLTTNEQLEAILFCYNFTKRFLEGFTDLIFRKVNDLYLLNA
KQNTLNDRIYNKIKLKLFDDTVYLMKKHIENRLNLDLFHKLKNELLSHIKYSKYAALAHD
QFVNEEELNAYLKSCTSGIDGFKELYDLKTKEQLVQWLAKGEIFKLAQIKNLNILEELLK
RK