GCW_02200

Uniprot: A0A0F6CKP5
Description: glycyl-tRNA synthetase
EC number: 6.1.1.14
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00015999}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the attachment of glycine to tRNA(Gly). {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00345537}.
Pathway:
Active site:
Binding site: BINDING 98 98 Substrate. {ECO:0000256|HAMAP-Rule:MF_00253}.; BINDING 170 170 Substrate. {ECO:0000256|HAMAP-Rule:MF_00253}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 202 204 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 212 217 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 287 288 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 331 334 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.
Site:
Gene names (primary): glyS
Gene names (synonym): glyQS
Mass: 54,264
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00006558}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; glycine-tRNA ligase activity [GO:0004820]; glycyl-tRNA aminoacylation [GO:0006426]
Gene ontology IDs: GO:0004820; GO:0005524; GO:0005737; GO:0006426
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00541124}.
Protein families: Class-II aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]: DOMAIN 119 373 AA_TRNA_LIGASE_II. {ECO:0000259|PROSITE:PS50862}.
Motif:
Region: REGION 217 221 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00253}.; REGION 327 331 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00253}.
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99667
UniPathway: K01880
CDD:
Gene3D: cd00774
HAMAP: 3.40.50.800
InterPro: MF_00253_B
PANTHER: IPR002314;IPR006195;IPR004154;IPR027031;IPR022961;IPR033731;IPR002315
PIRSF: PTHR10745
PRINTS:
PROSITE: PR01043
Pfam: PS50862
ProDom: PF03129;PF00587
SMART:
SUPFAM:
TIGRFAMs: SSF52954
518036-519428(-)

>nucleotide sequence
TTACTCATGCAAATTATTTCTAATTCATTGCTTTAATTCAGAGATTGGCAATTTAATTTG
TTCCATACTGTCACGTTCTCTGATCGTGACAATACCTTTTTCTAACGAATCAAAATCGTA
AGTTAAACAGTATTTTGTGCCGATTGCATCATATCTACGGTATCGCTTACCGATCGATCC
AGCGCTATCAAAATCAAATCTTAAGTTTGTATCACTGATCTTTAATAACTCTAGATATAG
TTGATAAGCTTGATCTTTTAGTTTATTAACTAATGGTAAGATTGCTAACTGATAAGGTGA
TAATGAAATGGGTAATCTTAAGACTTCTCTACTATCATTATCAGCTAATTGTTCGACATG
ATAATGAGCACAAACAATCGCATATAAGATTCGTTCGATCCCTAAAGAAGGTTCAATCAC
ATCAGGGATAATCTTAGTTTTTTCTATCTCATTATGATAATCAAGGGTTTTACCTGATTG
ATTTGAATGAGCAGTTAGATCAAAATTACCACGGTGAGCTAACCCTCATAATTCAGATCA
ACCATGAGGGAAATTAAATTGGAAATCGATCGTTCGAGATGAATAGTGAGCTAATTCGTC
TTTAGCAATCTCATGACGTTTAATTAGTTCTTTATTAATTAATAATTTATTAATTAAAAA
ATCTTCAATCTGGTTGATGTATCAATCAAACTTTTCTTTAACTAATTCAGGTTCTAGGAA
ATATTCAATCTCAAATTGTTCAAACTCTCTTGTTCTAAAGATGAAATTACCCGGGGTGAT
CTCATTTCTAAACGCTTTACCAATTTGTGCTACTCCAAACGGTAATTTCATTCTTTGGGT
TCTAAGAATATTCTTAAAGTTAATAAAGATCCCTTGAGCTGTTTCAGGTCTTAAATAAAT
TGTGTTAAGTGAATCTTCAACAACACCTTGAAACGTCTTAAACATCAGATTGAACTTTCT
GATTTTAGTTCAATTAGCTGCTTCACACTTCTTACATTCAACCTTCTTATCAACTAAGAT
CTGTTCTAAATAACTAGGGTCAGTATTTTCAGTGATCTTAATTGAATCATCATTCAATTC
TTCAATTAACTTATCAGCTCGATAACGTGATTTACATTCTTTACAATCAACTAGTGGATC
AGAAAAATTATCTAAATGACCTGATGCTTGTCACACTAAAGGATTTAAGATAATTGAGCT
GTCTAATAGTTTCATATTAGGTTGAGAAAACACGAAATGTTTTAACAACAACTGCTTAAT
ATTATTTTTTAATAAAGCTCCTAAAGGCCCATAGTCCCATGAGTTGGCTAACCCATTATA
AATCTCGGAAGACTGATAAACAAAGCCATAGTTCTTTAAGAAATTAACAATCTGATCTTG
TTTTAATTCCAT

>protein sequence
MELKQDQIVNFLKNYGFVYQSSEIYNGLANSWDYGPLGALLKNNIKQLLLKHFVFSQPNM
KLLDSSIILNPLVWQASGHLDNFSDPLVDCKECKSRYRADKLIEELNDDSIKITENTDPS
YLEQILVDKKVECKKCEAANWTKIRKFNLMFKTFQGVVEDSLNTIYLRPETAQGIFINFK
NILRTQRMKLPFGVAQIGKAFRNEITPGNFIFRTREFEQFEIEYFLEPELVKEKFDWYIN
QIEDFLINKLLINKELIKRHEIAKDELAHYSSRTIDFQFNFPHGWSELWGLAHRGNFDLT
AHSNQSGKTLDYHNEIEKTKIIPDVIEPSLGIERILYAIVCAHYHVEQLADNDSREVLRL
PISLSPYQLAILPLVNKLKDQAYQLYLELLKISDTNLRFDFDSAGSIGKRYRRYDAIGTK
YCLTYDFDSLEKGIVTIRERDSMEQIKLPISELKQWIRNNLHE