GCW_02270

Uniprot: A0A0F6CKQ6
Description: 4'-phosphopantetheinyl transferase
EC number: 2.7.8.7
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. {ECO:0000256|HAMAP-Rule:MF_00101, ECO:0000256|SAAS:SAAS00237157}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00101, ECO:0000256|SAAS:SAAS00245555};
Enzyme regulation:
Function [CC]: FUNCTION: Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein. {ECO:0000256|HAMAP-Rule:MF_00101, ECO:0000256|SAAS:SAAS00237197}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 8 8 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00101}.; METAL 57 57 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00101}.
Nucleotide binding:
Site:
Gene names (primary): acpS
Gene names (synonym):
Mass: 12,870
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; holo-[acyl-carrier-protein] synthase activity [GO:0008897]; magnesium ion binding [GO:0000287]; fatty acid biosynthetic process [GO:0006633]
Gene ontology IDs: GO:0000287; GO:0005737; GO:0006633; GO:0008897
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the P-Pant transferase superfamily. AcpS family. {ECO:0000256|HAMAP-Rule:MF_00101, ECO:0000256|SAAS:SAAS00563333}.
Protein families: P-Pant transferase superfamily, AcpS family
Coiled coil:
Domain [FT]: DOMAIN 4 76 ACPS. {ECO:0000259|Pfam:PF01648}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99678
UniPathway: K00997
CDD:
Gene3D:
HAMAP: 3.90.470.20
InterPro: MF_00101
PANTHER: IPR008278;IPR002582;IPR004568
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF01648
SMART: PD004282
SUPFAM:
TIGRFAMs: SSF56214
533053-533389(-)

>nucleotide sequence
TTAAATTCTTTCAGCTACTGCCATTGCTACTAATCTTTGACCTTCGTGAGAGATAGAAAG
GTGTAGCTTAATTTTTTCGTTTAAAGAGCATTCATAAACGCCGTGAATTTTACGAATTTC
TAATTGAGTGAATTCATCTCAAGTCTTAAGCGCTTTAAAAATCGCTTCTTTTAGTGATCA
TCTGATCGCTAAGAAGATGTTTTGGTTTGATTCACCAATAACAGTCTTATATTTGTTTTG
TTCGTGTTCTGTTAAAAGTTTCTTGATAAATTCATCAGATTTCTTAGCTTGAAATCTATC
AATACTTACAACGTCAATACCAACACCGATTATCAT

>protein sequence
MIIGVGIDVVSIDRFQAKKSDEFIKKLLTEHEQNKYKTVIGESNQNIFLAIRWSLKEAIF
KALKTWDEFTQLEIRKIHGVYECSLNEKIKLHLSISHEGQRLVAMAVAERI