GCW_02485

Uniprot: A0A0F6CKT0
Description: leucyl-tRNA synthetase
EC number: 6.1.1.4
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). {ECO:0000256|HAMAP-Rule:MF_00049}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site: BINDING 586 586 ATP. {ECO:0000256|HAMAP-Rule:MF_00049}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): leuS
Gene names (synonym):
Mass: 94,158
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; aminoacyl-tRNA editing activity [GO:0002161]; ATP binding [GO:0005524]; leucine-tRNA ligase activity [GO:0004823]; leucyl-tRNA aminoacylation [GO:0006429]
Gene ontology IDs: GO:0002161; GO:0004823; GO:0005524; GO:0005737; GO:0006429
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00049, ECO:0000256|RuleBase:RU363035}.
Protein families: Class-I aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]: DOMAIN 224 393 tRNA-synt_1_2. {ECO:0000259|Pfam:PF13603}.; DOMAIN 517 613 tRNA-synt_1. {ECO:0000259|Pfam:PF00133}.; DOMAIN 657 737 Anticodon_1. {ECO:0000259|Pfam:PF08264}.
Motif: MOTIF 39 50 "HIGH" region. {ECO:0000256|HAMAP-Rule:MF_00049}.; MOTIF 583 587 "KMSKS" region. {ECO:0000256|HAMAP-Rule:MF_00049}.
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99702
UniPathway: K01869
CDD:
Gene3D:
HAMAP: 1.10.730.10;3.40.50.620;3.90.740.10
InterPro: MF_00049_B
PANTHER: IPR001412;IPR002300;IPR002302;IPR025709;IPR013155;IPR015413;IPR014729;IPR009080;IPR009008
PIRSF: PTHR11946:SF7
PRINTS:
PROSITE: PR00985
Pfam: PS00178
ProDom: PF08264;PF00133;PF13603;PF09334
SMART:
SUPFAM:
TIGRFAMs: SSF47323;SSF50677
594318-596736(+)

>nucleotide sequence
ATGTACAACCATAATTTAATTGAAAAAAAATGAGCTAAGATCTGAAATGATCAGAAAGTT
TATTCTTTTCAGATTGATAAGAATAAACCTAAATATTACATCCTTGATATGTTTCCCTAT
CCTAGTGGAAAAGGTTTACACGTTGGTCATGTTAAAGCTTATATGGCCACTGATGTGATC
TCAAGATGGAAAAACGCTTTAGGGTTTAATGTTTTACACCCCATTGGTTGGGACGCTTTT
GGTTTACCAGCTGAACAGTATGCGATTCAAACTAATAACCATCCAACTAAATTTACCCAA
GAAAATATTAATAACTTCAGAACGCAATTAAAACGACTTGGGTTTAATTATGATTATCGT
CTAGAAGTAGATACAACGAATAAAAACTACTTTAAATGAACCCAATGGATCTTTAAAAAG
TTGTATGAACACGATCTAGCTTACCAAGCTGATATCGAAGTTAACTGGTGTGAACAATTG
GGAACGGTTTTAGCTAATGAAGAAGTACTAACTGATGAAAACGGGAATAAGATCTCTGAA
CGAGGATCTTATCCCGTAATTAAGAAAAAGATGCGCCAGTGAGTGTTGAAGATTACGGCT
TTTGCTGATCAGTTAATCGATGGTTTAGAGAACTTAAACTGACCTAATTCAATTAAGGCG
ATGCAAGTTAATTGAATTAATAAATCAGTTGGAGCAAGTATTAAATTTGAAATCGATCAG
TTAGACAACCAAACGATCGAAGTATTTAGTTCACGAGTTGATACGTTATTTGGTGCTAGT
TTTCTTGCATTAAGTTTTGATCATCCATTAGTTAAGCAAAAACTAATCACAGATAAAAAT
GACGCGATCGAACGATTTATTAAAGATAATAGTATTGATCAAACAGTAAGATATCAAGGG
ATCAATACTAATTATTTTGCGATCCACCCAATTACCAAGAAAAAGATCCCGATCTATTTG
GCTGATTACATCCTCAGTGATTATGGTACTGGAGCAGTGATGGGTGTACCAGCTCATGAT
GAAAGGGATTATCAGTTTGCCAAACAATATGATCTAGAGATCATCCCTGTGATTAAGGCT
GATCAATACCCTTATCTATTAGATGGTGAACATATTAACTCTGATTTTAATAACGGGTTA
AATAATGAACAAGCGATCCAAAAAACAATCGCTTATTTAAAGAAACATAATCTTGGTGAT
CAGAAGATTAATTACAAATTACGTGATTGGATCTTCTCACGCCAAAGATACTGGGGTGAA
CCTTTCCCAGTTTTATTTGATGAAGAAGATAATATCTATTTATTAAAAGATAGTGAACTA
CCACTAGAACTACCCCAATTAAGTGATTTCTCACCAAATAAAGATGGCTTGCCACCACTT
GCTAATGCAGATGATCAATGATTGCACCCAATCATTGATCAGAAGAAATACCGTCGTGAG
ATCAACACGATGCCCCAATGAGCTGGGTCTTGTTGGTATTATTTAGCTTACTTATTAAAG
TTAACTGATCTAAATCAAGCTGATGGTGATCAAAACTATTTAGCTTTAAATAGTGAAAAA
GCTAAAGAACTATTTGATCACTTTATGCCGGTTGATTTATATGTTGGTGGGCAAGAGCAT
GCAGTTTTACACTTATTATATGCAAGATTTTGGTATAAATTCTTACATCAGATCAAGATC
GTTAGTTCAACTGAACCGTTTAGTAAACTAATCAACCAAGGGATGATCTTGGGTGAAGAT
AATACCAAGATGTCAAAATCAAAAGGGAATATTATCAACCCAGATGATTTAGTTTTATCT
CATGGAGCTGATACGATTAGAACATATGTGATGTTTATGGGTCCACTTAATGCTAGTCTA
GCTTGGAACTCAAACGCACTAAATGGTACTAGAAAGTTTTTAGAACGAGTTTATAACTTA
TTTGATCGAGTGGAAATTAATGATTCAATTAATCAAAACCTAAATTATGATTATCATAAT
TTCTTAAAGAAGATAAACAAACATCTAGAAAACTTCGAATTCAACTTAGTTGTCAGTGAG
ATGATGATCTTTATTAATGCGTGTTATAAGCAAACTCAGATTAATAAGGAGATGATCACT
AACTTCTTAATCGTCTTATCATTCTTTGCTCCTTATTTAGCTGAAGAACTAAATAGTAAA
CTCAATAACCCAACCTTACTATATAAGATGAGATTAGCTCAATGAGATGAAGCTTATTTA
GTTAAAAACACAACAACGATCTCATGTAGTATTAATGGTAAGTTTAAATTAGTTCATGAG
TTTGATCTTGATAGTGATGAACAAGAAGTAGCCAACTACTTCTTGAATCAAGATCTAATC
AAAAGAAATCTAGAAGACAAGAAGTTAGTTAAAACAATCTTTGTGAAAAATAAAGTAATT
AACTTCATAATTAAATAA

>protein sequence
MYNHNLIEKKWAKIWNDQKVYSFQIDKNKPKYYILDMFPYPSGKGLHVGHVKAYMATDVI
SRWKNALGFNVLHPIGWDAFGLPAEQYAIQTNNHPTKFTQENINNFRTQLKRLGFNYDYR
LEVDTTNKNYFKWTQWIFKKLYEHDLAYQADIEVNWCEQLGTVLANEEVLTDENGNKISE
RGSYPVIKKKMRQWVLKITAFADQLIDGLENLNWPNSIKAMQVNWINKSVGASIKFEIDQ
LDNQTIEVFSSRVDTLFGASFLALSFDHPLVKQKLITDKNDAIERFIKDNSIDQTVRYQG
INTNYFAIHPITKKKIPIYLADYILSDYGTGAVMGVPAHDERDYQFAKQYDLEIIPVIKA
DQYPYLLDGEHINSDFNNGLNNEQAIQKTIAYLKKHNLGDQKINYKLRDWIFSRQRYWGE
PFPVLFDEEDNIYLLKDSELPLELPQLSDFSPNKDGLPPLANADDQWLHPIIDQKKYRRE
INTMPQWAGSCWYYLAYLLKLTDLNQADGDQNYLALNSEKAKELFDHFMPVDLYVGGQEH
AVLHLLYARFWYKFLHQIKIVSSTEPFSKLINQGMILGEDNTKMSKSKGNIINPDDLVLS
HGADTIRTYVMFMGPLNASLAWNSNALNGTRKFLERVYNLFDRVEINDSINQNLNYDYHN
FLKKINKHLENFEFNLVVSEMMIFINACYKQTQINKEMITNFLIVLSFFAPYLAEELNSK
LNNPTLLYKMRLAQWDEAYLVKNTTTISCSINGKFKLVHEFDLDSDEQEVANYFLNQDLI
KRNLEDKKLVKTIFVKNKVINFIIK