GCW_02725

Uniprot: A0A0F6CKX2
Description: 6-phosphofructokinase
EC number: 2.7.1.11
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. {ECO:0000256|SAAS:SAAS00197186}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|SAAS:SAAS00610220};
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. {ECO:0000256|SAAS:SAAS00197172}.
Pathway: PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. {ECO:0000256|SAAS:SAAS00054603}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): pfkA
Gene names (synonym):
Mass: 36,664
Subunit structure [CC]: SUBUNIT: Homotetramer. {ECO:0000256|SAAS:SAAS00557934}.
Gene ontology (GO): cytoplasm [GO:0005737]; 6-phosphofructokinase activity [GO:0003872]; ATP binding [GO:0005524]; metal ion binding [GO:0046872]; fructose 6-phosphate metabolic process [GO:0006002]
Gene ontology IDs: GO:0003872; GO:0005524; GO:0005737; GO:0006002; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the phosphofructokinase type A (PFKA) family. {ECO:0000256|SAAS:SAAS00557943}.
Protein families: Phosphofructokinase type A (PFKA) family
Coiled coil:
Domain [FT]: DOMAIN 7 286 PFK. {ECO:0000259|Pfam:PF00365}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99744
UniPathway: K00850
CDD:
Gene3D:
HAMAP:
InterPro:
PANTHER: IPR022953;IPR012003;IPR000023
PIRSF:
PRINTS: PIRSF000532
PROSITE: PR00476
Pfam:
ProDom: PF00365
SMART:
SUPFAM:
TIGRFAMs: SSF53784
645429-646428(-)

>nucleotide sequence
TTAAAAACCACCGTTTATTCTATTGCTTAAATTAATTAATTTGATTCTTGAAGGATTACG
TTTATTTAGTGCTTCTTGGAAATTATAAGCTACGATCTCATCCCCACTTGAACCTAAAAC
AACGCCGTATCTTTGTTCGTTAATACATTCAAACGCTTTGATCGCAAATTTCATGGCTAG
AATCCGTTCCATCGGTGTGGGAACTTGACCACGTTGACCGTAGGTTAAGATATTAAGATT
AGTTTTAATTCCTAACTGATCATTAATCTGTTGGGCGATCGTTTTTAAACTATCCCTGCC
ATCTTCACCATAAATATGTTCACAAACGATCACAGTAAATGTGCGTTTGTGATCTTGTAA
CATTCTTTTGGCAGCAAGATCTACGATCTCTTGGGTTGTTTTGATATTTTCTGGGGTGAT
TACTAGATCAGCTTTAGTTGCAAGTGCTGCATAAACTGCTAAATCCCCACAATGACGTCC
CATCACTTCAGAGATCGATACCCTGTTGTGAGAAACACTAGTTGAGATGATCTCTTGAAT
CGTTCTAGTAATATGTTCTAGTGCTGAATAAAAACCGATTGTGTAATCTGATGAAGAGAC
ATCGTTATCAATCGTTCCAGGCATCGCAATCACATTAATCCCCATCTCAGATAATAGTTT
TGCGCCTTGATATGAACCATCACCACCGATCACTACTAACGTATCGATTTTTTTTGCTTT
AAGATTACTTACTGCTTTTTTTCTTACTTCAAGATCTTTAAATTCAACTAATCTTGCTGA
ATAAATAAATGTTCCCGAATCAAAGAAATGGTTAATCACTTCTTGTTGGTTGGCAAGAAC
GATCTGATCTTCAACTAAGCCTTTATAACCTTCATAAACTAAGTAAGGTTGAATATTATT
AATGATTGCTTGTTGAACTAACCCATAAATTGCTGGATTCATTCCTGGAGAATCTCCTCC
AGAAGTTAAGATCGCAATTCGTTGTGTTTGAATGGCCAT

>protein sequence
MAIQTQRIAILTSGGDSPGMNPAIYGLVQQAIINNIQPYLVYEGYKGLVEDQIVLANQQE
VINHFFDSGTFIYSARLVEFKDLEVRKKAVSNLKAKKIDTLVVIGGDGSYQGAKLLSEMG
INVIAMPGTIDNDVSSSDYTIGFYSALEHITRTIQEIISTSVSHNRVSISEVMGRHCGDL
AVYAALATKADLVITPENIKTTQEIVDLAAKRMLQDHKRTFTVIVCEHIYGEDGRDSLKT
IAQQINDQLGIKTNLNILTYGQRGQVPTPMERILAMKFAIKAFECINEQRYGVVLGSSGD
EIVAYNFQEALNKRNPSRIKLINLSNRINGGF