GCW_02825

Uniprot: A0A0F6CKZ1
Description: thiamine biosynthesis protein ThiI
EC number: 2.8.1.4
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. {ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00498918}.; CATALYTIC ACTIVITY: [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. {ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00498909}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. {ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00498907}.
Pathway: PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis. {ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00498911}.
Active site:
Binding site: BINDING 264 264 ATP. {ECO:0000256|HAMAP-Rule:MF_00021}.; BINDING 286 286 ATP; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00021}.; BINDING 295 295 ATP. {ECO:0000256|HAMAP-Rule:MF_00021}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 180 181 ATP. {ECO:0000256|HAMAP-Rule:MF_00021}.; NP_BIND 205 206 ATP. {ECO:0000256|HAMAP-Rule:MF_00021}.
Site:
Gene names (primary): thiI
Gene names (synonym):
Mass: 45,642
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; sulfurtransferase activity [GO:0016783]; tRNA adenylyltransferase activity [GO:0004810]; tRNA binding [GO:0000049]; thiamine biosynthetic process [GO:0009228]; thiamine diphosphate biosynthetic process [GO:0009229]; tRNA thio-modification [GO:0034227]
Gene ontology IDs: GO:0000049; GO:0004810; GO:0005524; GO:0005737; GO:0009228; GO:0009229; GO:0016783; GO:0034227
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the ThiI family. {ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00543539}.; SIMILARITY: Contains 1 THUMP domain. {ECO:0000256|HAMAP-Rule:MF_00021}.
Protein families: ThiI family
Coiled coil:
Domain [FT]: DOMAIN 62 162 THUMP. {ECO:0000259|PROSITE:PS51165}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99763
UniPathway: K03151
CDD:
Gene3D:
HAMAP: 3.40.50.620
InterPro: MF_00021
PANTHER: IPR014729;IPR020536;IPR004114;IPR003720
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51165
ProDom: PF02568;PF02926
SMART:
SUPFAM: SM00981
TIGRFAMs:
670064-671255(-)

>nucleotide sequence
CTAATTGTTGTCTCATTGTTTATTTAAATCAAATTGAATAATATCGTTAATAATTGAATT
ATCAATAACTACATCTAAAAAATCTAATTTAGCTTCGATCTTATCGATAATTTCAATCCT
TGGGTTGGTAATTGGTTTTTTAGGCGCATATAATGAACAAGCATCAGAGTAAGGTAAGAT
CGAAATCTCATATGTTTTGATCTTCTTGGCGTGTTCAATGATTTGGCTTTTATCATAACA
CAATAAAGGCCGTAAAACTAATAAATCTTTGCAGGCATTTGAGATTACTTTCATACTGTT
GATCGTTTGTGAAGCAACTTGACCAAGTGATTCACCAGTAGCAATGCAGTCATACCCATA
CTGATTTATTAGTTTATTAGCAATCTTATAAAAACAACGACGCATTAATGTGATTCGGTA
GTTTTCATATTTGGTGTGTGAAATCTCTTTGAGCACATTGGTGAAGTTATGAATAAAGAT
CTTAGCATCGCTTACTTCGTTGTGCTTACTAACAGTTTGGGCTAATAATACGGTTTTATC
TAAAGCTTGCTTTGAAGTGTGTGGAGGAGTAATAAACGTTAAGAAATCAACGTGCATTCC
CCTTTTATAAAGTAGATCACTAGCAACAGGTGAGTCGATCCCACCTGATAATAGAACTAA
GACTTTGCCTGAAGATTTCACTGGTAACCCACCAGCCGCACTATATTTGTGCAAGTATAA
AATGAAATAATTCTTGACTACGTCAATACTAATCATTTTCGTTGGATTGGTCAGATTAAT
CTTAATCTGACGGTTTTTTAAACAATAAGCTGATAAGGTCTTTTTTAAATCAAGGCTGGT
AAGTTCATAACTTTTATCTTTTCTTTTGATCTCAAAAGCTAATTGATCATGATCATTAAC
CATCTCTAGAACTAAATCTTGTAACAGATTTAGATCACGTTGTAATCGTTGAACTAAACA
AACATAACTAATCCCAGGGATTCTTTTTAAGATCTCGATCAGTTCAGTTTGATCTTGCTT
GTGGTAGTTCGTGATTAAAAAATGATCGTATTGTAAATTGAGCTTTAACTCAAATTTACT
AATCGCTTGTTGTAGATTAGCCTTTAATTGGTTAATAAAGTTGATCCTGTTGTTATTTTT
TAGTCATAACTCACCGAATTTGATCATGATCTGATAGTTGCTAGTATTCAT

>protein sequence
MNTSNYQIMIKFGELWLKNNNRINFINQLKANLQQAISKFELKLNLQYDHFLITNYHKQD
QTELIEILKRIPGISYVCLVQRLQRDLNLLQDLVLEMVNDHDQLAFEIKRKDKSYELTSL
DLKKTLSAYCLKNRQIKINLTNPTKMISIDVVKNYFILYLHKYSAAGGLPVKSSGKVLVL
LSGGIDSPVASDLLYKRGMHVDFLTFITPPHTSKQALDKTVLLAQTVSKHNEVSDAKIFI
HNFTNVLKEISHTKYENYRITLMRRCFYKIANKLINQYGYDCIATGESLGQVASQTINSM
KVISNACKDLLVLRPLLCYDKSQIIEHAKKIKTYEISILPYSDACSLYAPKKPITNPRIE
IIDKIEAKLDFLDVVIDNSIINDIIQFDLNKQWDNN