GCW_02980

Uniprot: A0A0F6CL15
Description: azoreductase
EC number: 1.7.-.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000256|HAMAP-Rule:MF_01216}; ; Note=Binds 1 FMN per subunit. {ECO:0000256|HAMAP-Rule:MF_01216}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity. {ECO:0000256|HAMAP-Rule:MF_01216, ECO:0000256|SAAS:SAAS00016147}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): azoR
Gene names (synonym):
Mass: 22,093
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01216, ECO:0000256|SAAS:SAAS00016163}.
Gene ontology (GO): electron carrier activity [GO:0009055]; FMN binding [GO:0010181]; FMN reductase activity [GO:0008752]; oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor [GO:0016652]; oxidoreductase activity, acting on other nitrogenous compounds as donors [GO:0016661]
Gene ontology IDs: GO:0008752; GO:0009055; GO:0010181; GO:0016652; GO:0016661
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the azoreductase type 1 family. {ECO:0000256|HAMAP-Rule:MF_01216, ECO:0000256|SAAS:SAAS00540904}.
Protein families: Azoreductase type 1 family
Coiled coil:
Domain [FT]: DOMAIN 3 171 Flavodoxin_2. {ECO:0000259|Pfam:PF02525}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99787
UniPathway: K01118
CDD:
Gene3D:
HAMAP: 3.40.50.360
InterPro: MF_01216
PANTHER: IPR003680;IPR029039;IPR023048
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF02525
SMART:
SUPFAM:
TIGRFAMs: SSF52218
711644-712238(-)

>nucleotide sequence
TTAGAAATTTTTTGCTTTTTCTTTAATAAGCGATAGATTAGGTTCTACAATTTCTTTTGG
TTGTTCGTTTAAGTATTCAACTTTTGTTCCTGCAACTTTGATACTGTCAACCTTAGCACC
AATGAATTCAAACACACCTTTTAGTAAAACTGTTACGTCAGCAAATGCGTATCAGCCTTC
AGGTGCACCTTGGGTGTTAATAATCATTACTTTTAAGTGATCCATTAAACCAATTGATCC
ACCTTTTTTTGAATATTTATAGCTAAAGGTTTTGTTAGCTACACAAATTTTATCAAGTCA
ATTCTTAAGAGTGGCTGGATAATTAAAATTAGTCATCGGAGCAATTACTACTAATTTATC
AACGCTTTTAATCTGGTTAATTAATGGGTCAACGTTTTCATCCTTAAAGTGTTCCTTAGA
ATTTTTAGAAGTTAAAGTCATTGATCCTTTATCTAATTCGTTTAGATCCAATCAGCTAAC
TTGATCAGATGGGTTTAGTTTTAAATATTCAGATTCGAATGTTTTTGCTAATTGATAAGA
AAAGCTAGCTTCGTTAGCTACTGGAGAAGCATTAATAAAAAGTACTTTGCTCAT

>protein sequence
MSKVLFINASPVANEASFSYQLAKTFESEYLKLNPSDQVSWLDLNELDKGSMTLTSKNSK
EHFKDENVDPLINQIKSVDKLVVIAPMTNFNYPATLKNWLDKICVANKTFSYKYSKKGGS
IGLMDHLKVMIINTQGAPEGWYAFADVTVLLKGVFEFIGAKVDSIKVAGTKVEYLNEQPK
EIVEPNLSLIKEKAKNF