GCW_03025

Uniprot: A0A0F6CL23
Description: elongation factor G
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. {ECO:0000256|HAMAP-Rule:MF_00054}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 17 24 GTP. {ECO:0000256|HAMAP-Rule:MF_00054}.; NP_BIND 81 85 GTP. {ECO:0000256|HAMAP-Rule:MF_00054}.; NP_BIND 135 138 GTP. {ECO:0000256|HAMAP-Rule:MF_00054}.
Site:
Gene names (primary): fusA
Gene names (synonym):
Mass: 76,696
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; translation elongation factor activity [GO:0003746]
Gene ontology IDs: GO:0003746; GO:0003924; GO:0005525; GO:0005737
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily. {ECO:0000256|HAMAP-Rule:MF_00054}.
Protein families: TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-G/EF-2 subfamily
Coiled coil:
Domain [FT]: DOMAIN 8 282 Tr-type G (guanine nucleotide-binding). {ECO:0000259|PROSITE:PS51722}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99795
UniPathway: K02355
CDD:
Gene3D: cd01434
HAMAP: 3.30.230.10;3.30.70.240;3.40.50.300
InterPro: MF_00054_B
PANTHER: IPR009022;IPR000640;IPR004161;IPR031157;IPR027417;IPR020568;IPR014721;IPR005225;IPR000795;IPR009000;IPR004540;IPR005517
PIRSF:
PRINTS:
PROSITE: PR00315
Pfam: PS00301;PS51722
ProDom: PF00679;PF14492;PF03764;PF03144
SMART:
SUPFAM: SM00838;SM00889
TIGRFAMs: SSF50447;SSF52540;SSF54211;SSF54980
720187-722269(-)

>nucleotide sequence
CTATGCTGTTTTATTCTTTTTAGCTTTGTCTTCAATAACCTTTTCAACAACTGATTTTGG
TGCTTCAGCGTAGTGTGAGAATTGCATGATGTAATTACCACGACCTTGGGTAAATGATCT
TAGATCAGTCGCATAACCAAACATCTCACTTAAAGGAACTTTAGCTTTAATTACTTGAAC
ATTATCACGTGATTCAGTTCCTTCAATCATTCCTCTTCTTGAAGAGATATCACCCATTGT
GTCTCCGAAGTACTGTTCAGGAACAGTTACTTCGATCGCCATAATTGGTTCTAATAATGC
TGGTTGACAACGTTTACCAGCTTCTTTTAATGCCATTGAAGCAGCAATCTTATAAGCCAT
TTCATTTGAGTCGACATCGTGGTATGAACCATCAAATAAACTAGCTTTAATATCGATCAT
CGGATAACCAGCTAATGGACCGTTGTTCATAGCAGCCTCTAGACCTGCTTTAACTGATTT
AATGTATTCTTTAGGAATCTTACCACCAACGATCTTATCTTCGAATTCAAAACCTTTATC
GTGGTTAGGTTCAAAAGTAATCACAACGTGACCGTATTGACCACGACCACCTGATTGCTT
AATGTATTTACCTTCAACATCAGCTTTCGCTTTTAAAGTTTCACGATAACTTACTTGTGG
AGCACCAACATTAACATCAACTTTAAATTCACGTCTTAAACGGTCAACTAAGATATCAAG
GTGTAATTCCCCCATCCCAGCAATAATCGTTTGACCAGTTTCTTCATCAGTGTATGTTCT
AAACGTTGGGTCTTCTTCAGCTAACTTAGATAATGAGATCCCCATCTTTTCTTGGTCAGC
TTTTGTCTTTGGTTCAACTGCTAATGAGATAACAGGTTGAGCAAAGGTCATTGATTCTAA
TGTTAGATTTTGGTTTTCTGAAGCGATTGTATCACCAGTTGTAGTATCTTTTAATCCCAC
GATCGCACAGATATCACCAGCGCGAATCTCATCGATCTCATCACGTTGCTGAGCGTGCAT
CTTCACAATTCTTGATACTCTTTCTTTCTTGTCTTTAGTGGTATTAATCACATAAGAACC
TGAAGTTAGAATACCAGAGTAAACTCGAACAAAGGTTAGACGACCAACAAACGGGTCAGT
AGCCACTTTAAATGCTAGACCAACAAATGGTGCATCATCACTAGTAGGGATCTGTACTTC
TTCACCAACTTTATAACCTTTTGCTGGAGGTACATCGATTGGTGAAGGTAAGTAGTCAAC
CACCGCATCTAATAAAGGTTTAACCCCTTTATTCTTAAATGCAGTACCACATAAAACTGG
GAATAAAGTTGATGAAACAACACCTTTTCTAATACAAGATTTAATCTCTGCTTTAGAGAT
CTCTTGACCATCTAGGTATTTTTCCATTACTGCTTCATCAAAAGTCACAATCTCATCTAA
CATATGATTGTGCGCTTTTTTTGCTTCTTCTAAGTATTCTGCAGGAATCTCTTTAGTTTC
ATAGTTTTCATTCTCACCACCATCAAAGAAGTAAGCTTTCATCTCAACTAAATCAATGAT
TCCAACAAAATCATTTTCAGCTCCAATTGGTAACTGGATCGGAACTGATTTAACACCTAG
TCTTGAGTGGATTGAGTCAACTGATCTTTGGAAGTTAGCCCCAGTTTTGTCCATCTTGTT
AACGAACACTATTCTAGGCACTTCGTATCGACTAGCTTGACGTCAAACTGTTTCAGTTTG
AGGTTCTACACCCATTTGGGCATCTAATACTGCTACTGCACCATCTAACACTCTTAGTGA
ACGTTCAACTTCAACCGTAAAGTCAACGTGTCCAGGAGTATCAATTAAATTAAGTTGGCA
GTCTTTTCAAGTAACTGAAGTCGCTGCAGATGTAATAGTAATCCCACGCTCTTTTTCTTG
GGCCATCCAGTCCATTACTGATGCCCCATCGTGAGTTTCACCGATCTTATGGGTTTTTCC
AGAATGGAATAAAATTCTTTCAGAAGTTGTTGTTTTACCAGCATCAATGTGCGCCATGAT
ACCGAAATTTCTGAACTTTTCTAAAGGATATTGTCTTGCCAT

>protein sequence
MARQYPLEKFRNFGIMAHIDAGKTTTSERILFHSGKTHKIGETHDGASVMDWMAQEKERG
ITITSAATSVTWKDCQLNLIDTPGHVDFTVEVERSLRVLDGAVAVLDAQMGVEPQTETVW
RQASRYEVPRIVFVNKMDKTGANFQRSVDSIHSRLGVKSVPIQLPIGAENDFVGIIDLVE
MKAYFFDGGENENYETKEIPAEYLEEAKKAHNHMLDEIVTFDEAVMEKYLDGQEISKAEI
KSCIRKGVVSSTLFPVLCGTAFKNKGVKPLLDAVVDYLPSPIDVPPAKGYKVGEEVQIPT
SDDAPFVGLAFKVATDPFVGRLTFVRVYSGILTSGSYVINTTKDKKERVSRIVKMHAQQR
DEIDEIRAGDICAIVGLKDTTTGDTIASENQNLTLESMTFAQPVISLAVEPKTKADQEKM
GISLSKLAEEDPTFRTYTDEETGQTIIAGMGELHLDILVDRLRREFKVDVNVGAPQVSYR
ETLKAKADVEGKYIKQSGGRGQYGHVVITFEPNHDKGFEFEDKIVGGKIPKEYIKSVKAG
LEAAMNNGPLAGYPMIDIKASLFDGSYHDVDSNEMAYKIAASMALKEAGKRCQPALLEPI
MAIEVTVPEQYFGDTMGDISSRRGMIEGTESRDNVQVIKAKVPLSEMFGYATDLRSFTQG
RGNYIMQFSHYAEAPKSVVEKVIEDKAKKNKTA