GCW_03105

Uniprot: A0A0F6CLQ1
Description: inorganic polyphosphate/ATP-NAD kinase
EC number: 2.7.1.23
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + NAD(+) = ADP + NADP(+). {ECO:0000256|HAMAP-Rule:MF_00361, ECO:0000256|SAAS:SAAS00549059}.
Cofactor: COFACTOR: Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000256|HAMAP-Rule:MF_00361, ECO:0000256|SAAS:SAAS00608905};
Enzyme regulation:
Function [CC]: FUNCTION: Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. {ECO:0000256|HAMAP-Rule:MF_00361}.
Pathway:
Active site: ACT_SITE 58 58 Proton acceptor. {ECO:0000256|HAMAP-Rule:MF_00361}.
Binding site: BINDING 63 63 NAD. {ECO:0000256|HAMAP-Rule:MF_00361}.; BINDING 150 150 NAD. {ECO:0000256|HAMAP-Rule:MF_00361}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 58 59 NAD. {ECO:0000256|HAMAP-Rule:MF_00361}.; NP_BIND 125 126 NAD. {ECO:0000256|HAMAP-Rule:MF_00361}.
Site:
Gene names (primary): ppnK
Gene names (synonym): nadK
Mass: 30,706
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; metal ion binding [GO:0046872]; NAD+ kinase activity [GO:0003951]; NAD metabolic process [GO:0019674]; NADP biosynthetic process [GO:0006741]
Gene ontology IDs: GO:0003951; GO:0005524; GO:0005737; GO:0006741; GO:0019674; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the NAD kinase family. {ECO:0000256|HAMAP-Rule:MF_00361, ECO:0000256|SAAS:SAAS00549087}.
Protein families: NAD kinase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHC00024
UniPathway: K00858
CDD:
Gene3D:
HAMAP: 2.60.200.30;3.40.50.10330
InterPro: MF_00361
PANTHER: IPR017438;IPR017437;IPR016064;IPR002504
PIRSF: PTHR20275
PRINTS:
PROSITE:
Pfam:
ProDom: PF01513
SMART:
SUPFAM:
TIGRFAMs: SSF111331
738924-739743(-)

>nucleotide sequence
TTAGTAAATGAACGTTTTTTGCAATCTTTGCATGTAGCTTCTAAGATCAGTTGTGGCAAC
ATAATCTGCTTGAGATCTTGTTGCACTAATCTCAATCGTCGTTGAACTAAGTCCTTGTCT
AATTACAGCTCCATCAGCCACAATTCTTGGACAAGCATCATGATCGCAGTAATTTTCTTT
AATCTCGATTCTAATCTTCGTATCAATCGGTAAGATAATTGGTGATTGAATCGTGGTAAA
ACTCGAATGTAATAATGGGTATAGTTCAACCATCTGGATCGCTTTAATCCTAGGGAATAA
GATTGCGCCATTTGCTGATTTATTAATCCCAGTAGATCCAGTGGTTGTTGAGATTAATAA
CCCCGTGCCCCTAAACTTTTGATAAAACTCATTATCAATAAAGATGTCATAACCATAAGC
GGTTGTCGAATTAATATTAAATTCGTTTAGAGCATGATGGATCTGATCATCGATCTGCAA
CCTAATAAAATCTAATTGCGTGTATTTTAAATGATCTAAATTATTGGCAATCTGATCAAT
GTTGGTTTCATTAAACGTCGTATAAAAACCCAGACTACCACCATTGATCCCAATGATCTT
TAAGCCAGCACGGTCATATTTGATTGCATTTTTAATAAAAGTGCCATCACCGCCATTAAT
AAATAAGTAGTCTGCTACAGTTGGATCATCAACTTCAACTAACTTTTTATTTAGTTCTTT
TTTTATTAACGGTTTTAGTGATTCTGATTTTGGTGCTAATGAAGAGATTAAATAATATGT
TTTATTCATCTTCTTTTCTTGAACTTGGTGAACTTTCAT

>protein sequence
MKVHQVQEKKMNKTYYLISSLAPKSESLKPLIKKELNKKLVEVDDPTVADYLFINGGDGT
FIKNAIKYDRAGLKIIGINGGSLGFYTTFNETNIDQIANNLDHLKYTQLDFIRLQIDDQI
HHALNEFNINSTTAYGYDIFIDNEFYQKFRGTGLLISTTTGSTGINKSANGAILFPRIKA
IQMVELYPLLHSSFTTIQSPIILPIDTKIRIEIKENYCDHDACPRIVADGAVIRQGLSST
TIEISATRSQADYVATTDLRSYMQRLQKTFIY